Rapid Separation Technique for the Determination of Potential Impurities Present in Levodopa, Carbidopa, and Entacapone in Fixed Dose Combination Drug Product Using Trifunctionally Bonded Phase Ethylene Bridged Sorbent Column with Smaller Ion-Pair Reagent

Mantena, Bhaskara P. V.; Rao, Sumathi V.; Rao, K. M. Ch. Appa; Ramakrishna, K.; Reddy, R. Srikanth

doi:10.1080/10826076.2015.1020166

Cited by 5 publications

(2 citation statements)

References 12 publications

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“…The advantages of chemometrics are as follows: i) To apply chemometrics, which is the application of software, mathematics, and statistics to chemistry, to the simultaneous determination of the active substances to be analyzed. Studies in the literature for the analysis of levodopa, carbidopa and entacapone are [10,11], [12,13] and [14,15], respectively. After the spectrophotometric method was applied to the drug tablet sample containing the active ingredients levodopa, carbidopa, and entacapone, the data obtained were evaluated by two different chemometric methods.…”

Section: Introductionmentioning

confidence: 99%

Chemometric Determination of Parkinson's Drugs Containing Multiple Active Substances

Arslan,

Pekcan

2024

Cumhuriyet Science Journal

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In this work, the active ingredients of Entacapone, Levodopa and Carbidopa in drug samples used in the treatment of Parkinson's disease were quantitatively determined by Ultraviolet Visible (UV-VIS) Spectroscopy and chemometrics. Firstly, the spectra of each drug active ingredient were taken individually and then synthetic mixtures identical to the drug sample were analyzed. In our method, validation parameters were calculated for each method. Percent (%) recoveries were found on average for both the synthetic mixture and the commercial sample. The recoveries were quantitative for each method. The accuracy of the methods was tested by applying ANOVA test to the results obtained from the PLS and PCR calibration methods. The developed methods are reproducible, sensitive, and accurate, and can be recommended for the analysis of drug samples containing Entacapone, Levodopa, and Carbidopa

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Section: Introductionmentioning

confidence: 99%

Chemometric Determination of Parkinson's Drugs Containing Multiple Active Substances

Arslan,

Pekcan

2024

Cumhuriyet Science Journal

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“…Many analytical methods have been reported for the determination of LD, CBD and EN and their related substances or metabolites in drug products or biological matrices using high‐performance liquid chromatography (HPLC) and different types of detectors such as electrochemical, UV, coulometric, and tandem mass spectrometry (MS/MS). Many studies have employed HPLC methods with UV detection for the individual or combined determinations of LD, CBD or EN in tablet form or as the active ingredient using a reversed‐phase (RP) stationary phase and different types of mobile phases including those containing ion‐pairing reagents 6–19 . These methods were mainly used to determine the active ingredients (LD, CBD and EN) and some related substances or metabolites.…”

Section: Introductionmentioning

confidence: 99%

A rapid liquid chromatography/tandem mass spectrometry method for simultaneous determination of levodopa, carbidopa, entacapone and their six related compounds in film‐coated tablets

Burmaoğlu

Aslan

2020

Rapid Comm Mass Spectrometry

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Rationale:A liquid chromatography/tandem mass spectrometry (LC/MS/MS) method has been developed and validated to determine levodopa, carbidopa, entacapone, and corresponding six related substanceslevodopa impurity B, levodopa impurity C, methyldopa, methylcarbidopa, entacapone impurity C, and entacapone impurity Ain film-coated tablets for the first time.Methods: Chromatographic separation was achieved with a gradient elution by using a C18 column, a mobile phase containing 0.5% formic acid in water and 0.5% formic acid in methanol. The mobile phase flow rate was 0.5 mL min −1 . The UV detector was set at 280 nm and the triple quadrupole mass spectrometer was used in multiple reaction monitoring (MRM) mode. Results:The limit of detection (LOD) and limit of quantification (LOQ) results were 1.3 and 3.94 ng mL −1 for levodopa impurity B; 5.26 and 15.9 ng mL −1 for levodopa impurity C; 0.833 and 2.53 ng mL −1 for methyldopa; 3.31 and 10.0 ng mL −1 for methylcarbidopa; 1.67 and 5.06 ng mL −1 for entacapone impurity C; and 0.61 and 1.86 ng mL −1 for entacapone impurity A. Conclusions:The method was rapid, linear, accurate, and reproducible. The LC/MS/MS method that was developed to determine the related substances and assay of levodopa, carbidopa, and entacapone can be used to evaluate the quality of regular samples in the pharmaceutical industry. It can be also used to test the stability of samples.

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Electrochemical platform for simultaneous determination of levodopa, acetaminophen and tyrosine using a graphene and ferrocene modified carbon paste electrode

et al. 2017

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Rapid Separation Technique for the Determination of Potential Impurities Present in Levodopa, Carbidopa, and Entacapone in Fixed Dose Combination Drug Product Using Trifunctionally Bonded Phase Ethylene Bridged Sorbent Column with Smaller Ion-Pair Reagent

Cited by 5 publications

References 12 publications

Chemometric Determination of Parkinson's Drugs Containing Multiple Active Substances

Chemometric Determination of Parkinson's Drugs Containing Multiple Active Substances

A rapid liquid chromatography/tandem mass spectrometry method for simultaneous determination of levodopa, carbidopa, entacapone and their six related compounds in film‐coated tablets

Electrochemical platform for simultaneous determination of levodopa, acetaminophen and tyrosine using a graphene and ferrocene modified carbon paste electrode

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