Rapid simultaneous determination of ephedrines, amphetamines, cocaine, cocaine metabolites, and opiates in human urine by GC–MS

Saito, Takeshi; Mase, Hiroyasu; Takeichi, Sanae; Inokuchi, Sadaki

doi:10.1016/j.jpba.2006.06.031

Cited by 54 publications

(36 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Extraction of drugs of abuse from the liquid phase was performed as reported by Castiglioni et al [13], modified as reported by Saito et al [14]. The following chemicals were purchased from Chemical Research 2000: (a) analytical standards: Benzoylecgonine, Amphetamine, Methamphetamine, 11nor-9carboxy-Δ9-THC, (b) derivatization reagents: MSTFA, (c) internal standards: Benzoylecgonine-d3, Amphetamine-d6, Methamphetamine-d9, 11nor-9carboxy-Δ9-THC-d3.…”

Section: Drugs Of Abusementioning

confidence: 99%

Mass balance of emerging organic micropollutants in a small wastewater treatment plant

Boni¹,

Sbaffoni²,

Tedesco³

et al. 2012

Water Pollution XI

View full text Add to dashboard Cite

Emerging organic micropollutants are compounds measured in water in μg/l or ng/l which may have long-term negative effects on both wildlife and humans.The purpose of this work was to perform a mass balance of emerging organic micropollutants on activated sludge tank and on secondary settling tank in a small wastewater treatment plant in the Municipality of Rome. The analytes monitored in this work were: Estrone (E1), 17β-Estradiol (E2) and 17α-Ethinylestradiol (EE2) for steroid hormones, 4-Nonylphenol (4-NP) and Bisphenol A (BPA) for substituted Phenols and Alkylphenols, Amphetamine (AM), Methamphetamine (MET), Benzoylecgonine (BEG) and 11-nor-Δ9-THC9carboxy (THC-COOH) for drugs of abuse.Mass balance on activated sludge tank showed that the emerging organic micropollutants removed were BPA (59%), EE2 (34%), AM (56%) and THC-COOH (35%). The processes occurring in the secondary settling tank were able to reduce 4-NP (26%), BPA (74%), E1 (82%), EE2 (93%), AM (44%), MET (80%), BEG (72%) and THC-COOH (39%). The residual amount of each substance was distributed between the effluent, the return sludge line and the excess sludge.Since the removal of emerging organic micropollutants was partial, the conventional wastewater treatment plant is not able to completely break down these substances. Accordingly, these compounds are found in water body potentially active and dangerous to wildlife and humans.

show abstract

Section: Drugs Of Abusementioning

confidence: 99%

Mass balance of emerging organic micropollutants in a small wastewater treatment plant

Boni¹,

Sbaffoni²,

Tedesco³

et al. 2012

Water Pollution XI

View full text Add to dashboard Cite

show abstract

“…The shorter time of preparation and the less amount of solvents required for analysis are the main advantages of silylation over acylation (Segura et al, 1998 Urine Cocaine, EME PFPA/PFPOH 12.5 -50 ng/mL (Saito et al, 2007) Sweat Cocaine, BE, EME, EDME BSTFA/TMCS 2.5 ng/patch (Brunet et al, 2008) Urine Cocaine, BE BSTFA/TMCS 3 -10 ng/mL (Brunetto et al, 2010) Adipose tissue Cocaine, BE, EME, CE BSTFA 5 -20 ng/g (Colucci et al, 2010) Hair Cocaine, CE MSTFA 0.08 -0.09 ng/mg (Merola et al, 2010) BE -benzoylecgonine; BSTFA -N,O-bis(trimethylsilyl)trifluoroacetamide; CE -cocaethylene; EDME -ecgonidine methyl ester; EEE -ecgonine ethyl ester; EME -ecgonine methyl ester; HFBA -heptafluorobutyric anhydride; HFIP -1,1,1,3,3,3-hexafluoroisopropanol; MSTFA -N-methyl-Ntrimethylsilyltrifluoroacetamide; MTBSTFA -N-methyl-N-t-butyldimethylsilyltrifluoroacetamide; PFPA -pentafluoropropionic anhydride; PFPOH -2,2,3,3,3-pentafluoro-1-propanol; TBDMCS -t-butyldimethylchlorosilane; TMCS -trimethylchlorosilane. Table 3.…”

Section: Derivatization Proceduresmentioning

confidence: 99%

“…For acylation of cocaine analytes, the haloalkylacyl derivates, particularly the fluorinated ones like pentafluoropropionic anhydride (PFPA) and heptafluorobutyric anhydride (HFBA), are widely applied to several biological matrices, like blood and urine (Aderjan et al, 1993;Cardona et al, 2006;Saito et al, 2007), and hair and tissues (Cardona et al, 2006;Jurado et al, 1995).…”

Section: Derivatization Proceduresmentioning

confidence: 99%

Chromatographic Methodologies for Analysis of Cocaine and Its Metabolites in Biological Matrices

Valente¹,

Carvalho²,

Bastos³

et al. 2012

Gas Chromatography - Biochemicals, Narcotics and Essential Oils

View full text Add to dashboard Cite

“…[14][15][16] All other methods described, including the GC-based ones, require sample pretreatment such as liquid-liquid extraction, solid-phase extraction and/or filtration, which is time-consuming and reduces the sample throughput . [8][9][10]17 The application of GC analysis to quantify ephedrines was first developed in 1977 by Lin et al 18 They used a specific electron-capture assay after a liquid-liquid extraction with benzene and trifluoroacetic acid (TFA) derivatives to detect pseudoephedrine and norpseudoephedrine. 18 Subsequently, the GC method to determine ephedrine was modified to reduce the toxicity of sample preparation and improve the sensitivity using toluene as the extraction solvent and trifluoroacetic anhydride (TFAA).…”

Section: Introductionmentioning

confidence: 99%

Fast Ephedrine Quantification by Gas Chromatography Mass Spectrometry

Carneiro¹,

Silva²,

Cavalcante³

et al. 2018

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

Ephedrines are widely used in therapy. Because of their stimulant properties, these substances are relevant in different forensic fields. At present, the state of the art for ephedrines quantification relay based on a liquid chromatography mass spectrometry, mainly because of the dilute-andshoot approach. Notwithstanding, several gas chromatography based methods have already been described, all of them include cleanup steps, with the potential disadvantage of incurring errors and increasing the workload. In this paper, a straightforward method for ephedrine quantification based on gas chromatographic mass spectrometry, without cleanup and based on Doehlert matrix optimization is presented. Only 10 µL of a urine sample is necessary and for N-methyl-N-(trimethylsilyl)trifluoroacetamide/N-methyl-bis-trifluoracetamide derivatives, the intermediate precision was 2.77% for ephedrine, 9.20% for cathine, 8.29% for norephedrine and 4.27% for pseudoephedrine. The limit of detection was 20 ng mL -1 for ephedrine, 30 ng mL -1 for cathine and 40 ng mL -1 for norephedrine and pseudoephedrine.

show abstract

Rapid simultaneous determination of ephedrines, amphetamines, cocaine, cocaine metabolites, and opiates in human urine by GC–MS

Cited by 54 publications

References 7 publications

Mass balance of emerging organic micropollutants in a small wastewater treatment plant

Mass balance of emerging organic micropollutants in a small wastewater treatment plant

Chromatographic Methodologies for Analysis of Cocaine and Its Metabolites in Biological Matrices

Fast Ephedrine Quantification by Gas Chromatography Mass Spectrometry

Contact Info

Product

Resources

About