Rapid Solid-Phase Radioimmunoassay for Staphylococcal Enterotoxin A

Abstract

A rapid solid-phase radioimmunoassay for staphylococcal enterotoxin A is described. The assay procedure requires 3 to 4 h for completion by using a competitive inhibition system in which the antibody is attached to bromacetyl cellulose particles. It is accurate to a level of 0.01 jg of enterotoxin A/ml in a variety of media such as ham, milk products, crab meat, custard, etc. No significant interference was found with any media or food product tested.

Combined multiplex loop-mediated isothermal amplification with lateral flow assay to detect sea and seb genes of enterotoxic Staphylococcus aureus

“…Until now, several methods have been developed to detect SEs, such as radioimmunoassay, chemiluminescence enzyme immunoassay and quartz crystal microbalance (Collins et al 1973;Singh et al 2010;Zhang et al 2013). Available commercially immunoanalytical kits include SET-RPLA (Thermofisher, Waltham, MA), RIDASCREEN â SET (R-Biopharm, Darmstadt, Germany), VIDAS â SET 2 (bioM erieux, Marcy-l'Etoile, France) and Tecra TM Staph enterotoxin visual immunoassay (3M, St. Paul, MN).…”

Nonspecific reactions of a commercial enzyme-linked immunosorbent assay kit (TECRA) for detection of staphylococcal enterotoxins in foods

“…To detect this low level of enterotoxin in 100 g of food (1 to 2 ng of enterotoxin per g), several sensitive detection methods have been employed: (i) a radioimmunoassay (6,8,17,22); (ii) an enzyme-linked immunosorbent assay (2,10,11,14,21), and (iii) a reversed passive latex agglutination assay (RPLA) (16,23,26,27). Among these assay techniques, some commercial kits are now available: four enzyme-linked immunosorbent assay kits (an enzyme immunoassay [EIA] kit made in Switzerland, a visual immunoassay [TECRA] kit made in Australia, and an ELISA membrane kit and an ELISA tube kit made in France) and one RPLA kit made in Japan.…”

Improved Reversed Passive Hemagglutination for Simple and Rapid Detection of Staphylococcal Enterotoxins A~E in Food

“…In routine practice, however, many researchers (1,2) pointed out the occurrence of false positives or cross-reactions among the toxin types. On the other hand, radio-immunoassay procedures reported by Johnson et al (10), Collins et al (5) and Robern et al (12) were capable of detecting enterotoxins in food homogenates without concentration, but they showed a 10-15% rate of false positives. Furthermore, the procedure is rather complicated and can not be used in many laboratories.…”