Rapid stimulation of pinocytosis in human carcinoma cells A-431 by epidermal growth factor.

Haigler, Harry T.; McKanna, J A; Cohen, Stanley

doi:10.1083/jcb.83.1.82

Cited by 315 publications

(223 citation statements)

References 28 publications

(43 reference statements)

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“…These contrasting results could be explained considering that the mechanisms that regulate endocytosis might differ in different cells. Supporting this possibility it has been described that, not only the routes for macropinocytosed Ags, but also the sensitivity of macropinocytosis to microtubule and microfilament destabilizing drugs differ between professional phagocytes and other cell types (38,59,(61)(62)(63). However, our observations made in bicarbonate-free medium support that, as observed in other cell types, acidification of cytosol exerts an inhibitory effect on DC endocytosis.…”

Section: Discussionsupporting

confidence: 88%

Acidosis Improves Uptake of Antigens and MHC Class I-Restricted Presentation by Dendritic Cells

Vermeulen

Giordano

Trevani

et al. 2004

The Journal of Immunology

115

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It is widely appreciated that inflammatory responses in peripheral tissues are usually associated to the development of acidic microenvironments. Despite this, there are few studies aimed to analyze the effect of extracellular pH on immune cell functions. We analyzed the impact of acidosis on the behavior of dendritic cells (DCs) derived from murine bone marrow. We found that extracellular acidosis (pH 6.5) markedly stimulated the uptake of FITC-OVA, FITC-dextran, and HRP by DCs. In fact, to reach similar levels of endocytosis, DCs cultured at pH 7.3 required concentrations of Ag in the extracellular medium almost 10-fold higher compared with DCs cultured at pH 6.5. Not only the endocytic capacity of DCs was up-regulated by extracellular acidosis, but also the expression of CD11c, MHC class II, CD40, and CD86 as well as the acquisition of extracellular Ags by DCs for MHC class I-restricted presentation. Importantly, DCs pulsed with Ag under acidosis showed an improved efficacy to induce both specific CD8+ CTLs and specific Ab responses in vivo. Our results suggest that extracellular acidosis improves the Ag-presenting capacity of DCs.

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Section: Discussionsupporting

confidence: 88%

Acidosis Improves Uptake of Antigens and MHC Class I-Restricted Presentation by Dendritic Cells

Vermeulen

Giordano

Trevani

et al. 2004

The Journal of Immunology

115

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“…When motility factors or IGF-I are added to MDCK cells, ruffling, which is associated with endocytosis, occurs (Kirkeeide et al 1993;Dowrick and Warn 1991). Similar patterns have been seen in fibroblasts (Dunlevy and Couchman 1993) and in bovine arterial smooth muscle cells (Davies and Ross 1980;Haigler et al 1979) after cytokine exposure. This is the first report of similar patterns in MCs.…”

Section: Discussionmentioning

confidence: 51%

Insulin-like Growth Factor I (IGF-I) Induces Unique Effects in the Cytoskeleton of Cultured Rat Glomerular Mesangial Cells

Berfield

Aspray

Abrass

1997

J Histochem Cytochem.

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SUMMARYResident glomerular mesangial cells (MCs) have complex cytoskeletal organizations that maintain functional and structural integrity. The ability of cells to replicate, coordinate movement, change shape, and interact with contiguous cells or extracellular matrix depends on cytoskeletal organization. MCs synthesize insulin-like growth factor (IGF-I), express IGF-I receptors, and respond to IGF-I with increased proliferation. We noted that IGF-I treatment of mesangial cells was associated with a change in morphology. Therefore, these studies were undertaken to define specific IGF-I-mediated changes in cytoskeletal protein organization. Rat MCs were propagated from birth in culture without supplemental insulin. Quiescent, subconfluent cultures were treated with IGF-I (100 nM) for 1 hr. Rearrangements in f-actin, ␣ -smooth muscle actin, ␤ -actin, vimentin, and vinculin were seen by fluorescence microscopy. As the cytoskeleton rearranged, ␣ -smooth muscle actin dissociated from the f-actin bundles and ␤ -actin became polymerized under the leading lamellar edge. Ultrastructural changes were consistent with increased membrane turnover and metabolic activity. The normally sessile mesangial cell was induced by IGF-I to express a woundhealing phenotype characterized by movement and increased pinocytosis. These changes are different from those induced by insulin and have important implications for mesangial cell function. ( J Histochem Cytochem 45:583-593, 1997)

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“…Active EGFR is taken into clathrin-coated pits from the plasma membrane, and may remain associated with its ligand until its degradation in lysosomes (47)(48)(49). The association of EGFR with CHC is liganddependent, and therefore is another assay of this receptor's activity (50).…”

Section: Evidence For Increased Internalization Of Egfr Confirms Thatmentioning

confidence: 99%

Apc Deficiency Is Associated with Increased Egfr Activity in the Intestinal Enterocytes and Adenomas of C57BL/6J-Min/+ Mice

Moran¹,

Hunt

Javid³

et al. 2004

Journal of Biological Chemistry

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Rapid stimulation of pinocytosis in human carcinoma cells A-431 by epidermal growth factor.

Abstract: The tumor-promoter 12-0-tetradecanoyl-phorbol-13-acetate, which shares several biological activities with EGF, was also effective in stimulating an increase in the rate of pinocytosis .

Cited by 315 publications

References 28 publications

Acidosis Improves Uptake of Antigens and MHC Class I-Restricted Presentation by Dendritic Cells

Acidosis Improves Uptake of Antigens and MHC Class I-Restricted Presentation by Dendritic Cells

Insulin-like Growth Factor I (IGF-I) Induces Unique Effects in the Cytoskeleton of Cultured Rat Glomerular Mesangial Cells

Apc Deficiency Is Associated with Increased Egfr Activity in the Intestinal Enterocytes and Adenomas of C57BL/6J-Min/+ Mice

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