Rapid Viral Diagnosis of Orthopoxviruses by Electron Microscopy: Optional or a Must?

Gelderblom, Hans R.; Madeley, Dick

doi:10.3390/v10040142

Cited by 30 publications

(39 citation statements)

References 95 publications

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“…Serum and swab specimens tested positive for orthopoxvirus but negative for variola virus on the BioFire FilmArray Biothreat Panel version 2.5 ( https://www.biofiredx.com ). We confirmed orthopoxvirus from swab specimen using a panorthopoxvirus PCR targeting E9L (DNA polymerase) ( 6 ) and by direct visualization of virus particles using transmission electron microscopy, which showed features characteristic of orthopoxviruses ( Figure 2 , panels A, B) ( 7 ).…”

Section: The Casementioning

confidence: 79%

Imported Monkeypox, Singapore

Yong¹,

Ng²,

Ho³

et al. 2020

Emerg. Infect. Dis.

237

198

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Section: The Casementioning

confidence: 79%

Imported Monkeypox, Singapore

Yong¹,

Ng²,

Ho³

et al. 2020

Emerg. Infect. Dis.

237

198

View full text Add to dashboard Cite

“…The hallmark of TEM is its “open view” nature, since it provides an immediate overview of actual status, discerning amount and shape of virus(es) present, including the unexpected (Gentile and Gelderblom, 2014; Gelderblom and Madeley, 2018). As a first step in pathogen recognition, it requires only minute amounts of samples carrying high virus loads.…”

Section: Routine Diagnosismentioning

confidence: 99%

Electron Microscopy Methods for Virus Diagnosis and High Resolution Analysis of Viruses

et al. 2019

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The term “virosphere” describes both the space where viruses are found and the space they influence, and can extend to their impact on the environment, highlighting the complexity of the interactions involved. Studying the biology of viruses and the etiology of virus disease is crucial to the prevention of viral disease, efficient and reliable virus diagnosis, and virus control. Electron microscopy (EM) is an essential tool in the detection and analysis of virus replication. New EM methods and ongoing technical improvements offer a broad spectrum of applications, allowing in-depth investigation of viral impact on not only the host but also the environment. Indeed, using the most up-to-date electron cryomicroscopy methods, such investigations are now close to atomic resolution. In combination with bioinformatics, the transition from 2D imaging to 3D remodeling allows structural and functional analyses that extend and augment our knowledge of the astonishing diversity in virus structure and lifestyle. In combination with confocal laser scanning microscopy, EM enables live imaging of cells and tissues with high-resolution analysis. Here, we describe the pivotal role played by EM in the study of viruses, from structural analysis to the biological relevance of the viral metagenome (virome).

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“…In the case of equine herpesvirus, there are fuzzy projections on the outer surface of the envelope, an ill-defi ned tegument underneath, and a tilted core. The genome appears as an electron-dense zone under thinsection transmission electron microscopy [20]. The electron microscopic technique enables the diff erentiation and identifi cation of viruses.…”

Section: The Structural Topographic and Biochemical Aspects Of The mentioning

confidence: 99%

Biophysical Aspects of Interactions at the Bionanointerface between Viruses and Metal and Metal Oxide Nanomaterials

Lahir¹

2020

J Biomed Res Environ Sci

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Viruses are at the threshold of living and nonliving entities. Virus particles exhibit lifeactivities when are within their respective hosts and act as non-living when present outside their hosts. This feature is very interesting and the related investigations can help to understand the differences between the functionalities at bionanointerfaces under living and nonliving phases. Metal and metal oxide nanomaterials occur naturally and are synthesized as per the need to meet the set targets. These nanosized materials have specifi c physicochemical properties such as high volume to area ratio, ability to get functionalized as per the need. These ubiquitous materials have multifaceted applications in almost all fi elds of sciences, industries, medical, clinical diagnostics, and remedial operations; these occupy an omnipresent status in our day to day life. Since these nanomaterials are a major integral part of industries and human life; these interact with the abiotic and biotic components of the environment. Viruses are the active entities of both these aspects of our environment. The interactions between metal and metal oxide nanomaterials and viruses are obvious and complex interactive phenomena. These complex interactions take place between nanomaterials and viruses within their respective hosts. The profi ling of such interactions helps to optimize the resultant impacts and enhances the degree of de novo designing, in vivo, and in vitro performances.

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Rapid Viral Diagnosis of Orthopoxviruses by Electron Microscopy: Optional or a Must?

Cited by 30 publications

References 95 publications

Imported Monkeypox, Singapore

Imported Monkeypox, Singapore

Electron Microscopy Methods for Virus Diagnosis and High Resolution Analysis of Viruses

Biophysical Aspects of Interactions at the Bionanointerface between Viruses and Metal and Metal Oxide Nanomaterials

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