Rare case of meningitis due to an emerging pathogen <i>Chryseobacterium indologenes</i>

Sud, Anuj; Chaudhary, Monica; Baveja, C.P.; Pandey, PN

doi:10.1177/2050313x20936098

Cited by 4 publications

(5 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although some studies have reported that C. indologenes remains susceptible to trimethoprim/sulfamethoxazole, quinolones, minocycline, cefepime, ceftazidime, piperacillin-tazobactam, and rifampin ( 10 , 18 , 81 , 82 ), our strain was resistant not only to piperacillin–tazobactam, ciprofloxacin, cefepime, and ceftazidime but also to colistin and tigecycline. Our findings are in accordance with other studies ( 3 , 9 , 24 ) that show an increasing trend in resistance of C. indologenes against most commonly used antimicrobial agents.…”

Section: Discussionsupporting

confidence: 93%

“…Although the clinical significance and pathogenicity of C. indologenes are not well established (17,18), C. indologenes is known to be naturally resistant to a wide variety of antibiotics including not only aminoglycosides, tetracyclines, chloramphenicol, macrolides, aminopenicillins, clindamycin, teicoplanin but also first-generation cephalosporins, aztreonam, ticarcillin-clavulanate, and carbapenems (10,(19)(20)(21). The most potent drugs reported against C. indologenes are quinolones (gatifloxacin and levofloxacin), minocycline, and trimethoprimsulfamethoxazole (18,22,23); however, many studies show an alarming trend in resistance to those antibiotics (3,9,24).…”

Section: Introductionmentioning

confidence: 99%

“…Chryseobacterium indolog enes is a non-motile, catalase-positive, oxidase-positive, indole-positive, non-glucose-fermenting Gram-negative bacilli ( 1 ) widely distributed in nature, but it is not normally present in the human microflora ( 2 , 3 ). In humans, C. indologenes was isolated for the first time from a tracheal aspirate of a patient with ventilator-associated pneumonia ( 4 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Whole genome sequencing of the multidrug-resistant Chryseobacterium indologenes isolated from a patient in Brazil

et al. 2022

View full text Add to dashboard Cite

Chryseobacterium indologenes is a non-glucose-fermenting Gram-negative bacillus. This emerging multidrug resistant opportunistic nosocomial pathogen can cause severe infections in neonates and immunocompromised patients. This study aimed to present the first detailed draft genome sequence of a multidrug-resistant C. indologenes strain isolated from the cerebrospinal fluid of an infant hospitalized at the Neonatal Intensive Care Unit of Brazilian Tertiary Hospital. We first analyzed the susceptibility of C. indologenes strain to different antibiotics using the VITEK 2 system. The strain demonstrated an outstanding resistance to all the antibiotic classes tested, including β-lactams, aminoglycosides, glycylcycline, and polymyxin. Next, C. indologenes was whole-genome-sequenced, annotated using Prokka and Rapid Annotation using Subsystems Technology (RAST), and screened for orthologous groups (EggNOG), gene ontology (GO), resistance genes, virulence genes, and mobile genetic elements using different software tools. The draft genome contained one circular chromosome of 4,836,765 bp with 37.32% GC content. The genomic features of the chromosome present numerous genes related to cellular processes that are essential to bacteria. The MDR C. indologenes revealed the presence of genes that corresponded to the resistance phenotypes, including genes to β-lactamases (blaIND–13, blaCIA–3, blaTEM–116, blaOXA–209, blaVEB–15), quinolone (mcbG), tigecycline (tet(X6)), and genes encoding efflux pumps which confer resistance to aminoglycosides (RanA/RanB), and colistin (HlyD/TolC). Amino acid substitutions related to quinolone resistance were observed in GyrA (S83Y) and GyrB (L425I and K473R). A mutation that may play a role in the development of colistin resistance was detected in lpxA (G68D). Chryseobacterium indologenes isolate harbored 19 virulence factors, most of which were involved in infection pathways. We identified 13 Genomic Islands (GIs) and some elements associated with one integrative and conjugative element (ICEs). Other elements linked to mobile genetic elements (MGEs), such as insertion sequence (ISEIsp1), transposon (Tn5393), and integron (In31), were also present in the C. indologenes genome. Although plasmids were not detected, a ColRNAI replicon type and the most resistance genes detected in singletons were identified in unaligned scaffolds. We provided a wide range of information toward the understanding of the genomic diversity of C. indologenes, which can contribute to controlling the evolution and dissemination of this pathogen in healthcare settings.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Whole genome sequencing of the multidrug-resistant Chryseobacterium indologenes isolated from a patient in Brazil

et al. 2022

View full text Add to dashboard Cite

show abstract

“…No hemolytic properties were observed. The hemolytic activities of Bacillus [Dabiré et al, 2022], Chryseobacterium [Sud et al, 2020], Mycobacterium [Augenstreich et al, 2020], and Stenotrophomonas genera [Peters et al, 2020] were reported. However, only Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Potential Virulence, Resistance to Antibiotics and Heavy Metals, and Biofilm-Forming Capabilities of Soil Lignocellulolytic Bacteria

Kognou¹,

Chio²,

Khatiwada³

et al. 2023

Microb Physiol

View full text Add to dashboard Cite

Soil bacteria participate in self-immobilization processes for survival, persistence and producing virulence factors in some niches or hosts through their capacities of autoaggregation, cell surface hydrophobicity, biofilm formation, and antibiotic and heavy metal resistance. This study investigated potential virulence, antibiotics and heavy metals resistance, solvent adhesion, and biofilm-forming capabilities of six cellulolytic bacteria isolated from soil samples: Paenarthrobacter sp. MKAL1, Hymenobacter sp. MKAL2, Mycobacterium sp. MKAL3, Stenotrophomonas sp. MKAL4, Chryseobacterium sp. MKAL5 and Bacillus sp. MKAL6. Strains were subjected to phenotypic methods, including heavy metal and antibiotic susceptibility and virulence factors (protease, lipase, capsule production, autoaggregation, hydrophobicity and biofilm formation). The effect of ciprofloxacin was also investigated on bacterial susceptibility over time, cell membrane and biofilm formation. Strains MKAL2, MKAL5 and MKAL6 exhibited protease and lipase activities, while only MKAL6 produced capsules. All strains were capable of aggregating, forming biofilm and adhering to solvents. Strains tolerated high amounts of chromium, lead, zinc, nickel and manganese and were resistant to lincomycin. Ciprofloxacin exhibited bactericidal activity against these strains. Although the phenotypic evaluation of virulence factors of bacteria can indicate their pathogenic nature, an in-depth genetic study of virulence, antibiotic and heavy metal resistance genes is required.

show abstract

“…C. indologenes was first isolated in 1993 from a tracheal aspirate in a patient with ventilator-associated pneumonia [ 2 3 ]. Although it rarely causes infections, manifestations such as bacteremia, pneumonia, intra-abdominal infection, skin and soft tissue infection, urinary tract infection, meningitis, keratitis, and catheter-related infections have been reported [ 4 5 6 7 8 9 10 11 12 13 14 15 16 ]. It can cause serious disease, especially in immunocompromised and critically ill patients and sometimes associated with prolonged indwelling devices ( e.g.…”

Section: Introductionmentioning

confidence: 99%

Clinical and Microbiological Characteristics ofChryseobacterium indologenesBacteremia: A 20-Year Experience in a Single University Hospital

et al. 2023

View full text Add to dashboard Cite

Background Chryseobacterium indologenes is ubiquitous in nature and rarely causes infections. However, the clinical impact of C. indologenes has increased in recent years, especially in immunocompromised patients, and has resulted in high mortality rates. We aimed to investigate the clinical and microbiological characteristics of C. indologenes bacteremia. Materials and Methods We retrospectively reviewed medical records of a 642-bed university-affiliated hospital in Korea, dating from January 2001 to December 2020, to investigate C. indologenes bacteremia. Results A total of 22 C. indologenes isolates were identified from blood culture records. All patients were hospitalized at the time of bacteremia, and the most common manifestation was primary bacteremia. A sizable majority of the patients (83.3%) had underlying diseases, and all patients received intensive care unit care during their admission. The 14-day and 28-day mortality rates were 8.3% and 16.7%, respectively. Importantly, all C. indologenes isolates were 100% susceptible to trimethoprim-sulfamethoxazole. Conclusion In our study, most of the infections were hospital-acquired, and the susceptibility pattern of the C. indologenes isolates showed multidrug resistance. However, trimethoprim-sulfamethoxazole is a potentially useful antibiotic for C. indologenes bacteremia treatment. More attention is required to identify C. indologenes as one of the most important nosocomial bacteria with detrimental effects in immunocompromised patients.

show abstract

Rare case of meningitis due to an emerging pathogen Chryseobacterium indologenes

Cited by 4 publications

References 16 publications

Whole genome sequencing of the multidrug-resistant Chryseobacterium indologenes isolated from a patient in Brazil

Whole genome sequencing of the multidrug-resistant Chryseobacterium indologenes isolated from a patient in Brazil

Characterization of Potential Virulence, Resistance to Antibiotics and Heavy Metals, and Biofilm-Forming Capabilities of Soil Lignocellulolytic Bacteria

Clinical and Microbiological Characteristics ofChryseobacterium indologenesBacteremia: A 20-Year Experience in a Single University Hospital

Contact Info

Product

Resources

About