Rare triterpene glycoside of ginseng (ginsenoside malonyl-Rg1) detected in plant cell suspension culture of Panax japonicus var. repens

Кочкин, Д. В.; Galishev, B. A.; Глаголева, Е.С.; Titova, M. V.; Носов, А. М.

doi:10.1134/s102144371705003x

Cited by 17 publications

(19 citation statements)

References 14 publications

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“…HPLC‐ESI‐MS analysis and mass spectrometry spectra showed that the Panax japonicus var. repens cells in vitro contain several isomers of malonyl‐Rg 1 (Kochkin, Galishev, Glagoleva, Titova, & Nosov, ). Under the present LC‐MS conditions, no new compounds were detected from ginseng tissue cultures.…”

Section: Discussionmentioning

confidence: 99%

“…ponicus var. repens cells in vitro contain several isomers of malonyl-Rg 1(Kochkin, Galishev, Glagoleva, Titova, & Nosov, 2017). Under the F I G U R E 2 Principal component analysis (PCA) score plots from five-year ginseng, three decades ginseng and AR lines (AR-5R, AR-5M, AR-4R, AR-4M, AR-R, and AR-M) F I G U R E 3 The heat map shows the relative expression levels of genes involved in ginsenosides biosynthesis in P. ginseng roots lines.…”

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Quality evaluation of Panax ginseng adventitious roots based on ginsenoside constituents, functional genes, and ferric‐reducing antioxidant power

et al. 2019

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In the study, six adventitious root lines of Panax ginseng have been successfully established. HPLC‐ESI‐MS analysis showed that 20 ginsenosides were identified in root lines, notoginsenoside Fa and notoginsenoside R2 were not found in AR lines. In AR lines, the highest accumulation of total ginsenosides was obtained in five‐year main AR (24.87 mg/g). Principal component analysis classified root lines into three groups. Five‐year ginseng was mostly similar with five‐year main AR, five‐year rootlet AR, and four‐year rootlet AR in ginsenosides composition of group 1. Besides, gene expressions were consistent with the production of total ginsenosides, and correlation analysis revealed that total ginsenosides biosynthesis was significantly positively correlated with the gene expression of dammarenediol synthase. Five‐year rootlet AR showed the highest activity on ferric‐reducing antioxidant power test among samples. It provides a scientific evidence for the further exploitation and large‐scale production of P. ginseng. Practical applications This study provides valuable information for the commercial scale culture of ginseng adventitious roots. This report combines morphology, ginsenoside composition and content, gene expression, and ferric‐reducing antioxidant power test to evaluate the quality of P. ginseng adventitious root, and combined with principal component analysis to screen out the high yield and stable ginseng adventitious roots. It would be profitable to use adventitious root culture of P. ginseng instead of field cultivation.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Quality evaluation of Panax ginseng adventitious roots based on ginsenoside constituents, functional genes, and ferric‐reducing antioxidant power

et al. 2019

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“… Panax japonicas var. repens – Ginsenosides: Rg1, Malonyl‐Rg1, Rb1, Malonyl‐Rb1, Rb2, Malonyl‐Rb2, Rc, Malonyl‐Rc, Re, Rd, Malonyl‐Rd, Rf, R0, Rh1, Rg2, Notoginsenoside R1, Chikusetsusaponin IVa, Zingibroside R1 [17,18] Dioscorea deltoidea – 25( S )‐Deltoside (25( S )‐protodeltonin); Deltoside (protodeltonin); 25( S )‐Protodioscin (protoneodioscin); Protodioscin [19,20] …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Antihypoxic Action of Panax Japonicus, Tribulus Terrestris and Dioscorea Deltoidea Cell Cultures: In Silico and Animal Studies

Lagunin

Повыдыш

Ivkin

et al. 2020

Molecular Informatics

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Chemical diversity of secondary metabolites provides a considerable variety of pharmacological actions with a significant extension due to their combinations in plant extracts. Production of plant‐derived medicinal products in cell cultures has advantages because of the efficient use of different biotic and abiotic elicitors and better control of the developmental processes. Using PASS software, we predicted biological activity spectra for phytoconstituents identified in cell cultures of Panax japonicus (12 molecules), Tribulus terrestris (4 molecules), and Dioscorea deltoidea (3 molecules). Mechanisms of action associated with the antihypoxic effect were predicted for the majority of molecules. PharmaExpert software allowed analyzing possible synergistic or additive effects of the combinations of phytoconstituents associated with the antihypoxic action. Experimental studies of the antihypoxic effect of the plants′ extracts in water and ethanol have been performed in 3 animal models: Acute asphyctic hypoxia (AAH), Acute haemic hypoxia (AHeH), and Acute histotoxic hypoxia (AHtH). Effects of Panax japonicus and Tribulus terrestris preparations exceeded the activity of the reference drug Mexidol in the AHtH model. In the AHeH model, all preparations demonstrated moderate activity; the most potent has been observed for Dioscorea deltoidea. Thus, we found that experimental studies in animal models have confirmed the in silico prediction.

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“…Подготовка проб для качественного анализа тритерпеновых гликозидов. Подготовку проб для качественного анализа тритерпеновых гликозидов в образцах корней женьшеня настоящего (навеска измельченных лиофильно высушенных корней -5 мг) осуществляли согласно опубликованной методике [5].…”

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Analysis of Gynzenosides in the Roots of Panax Ginseng Introduced in the Central Botanical Garden of Nas of Belarus

Кочкин¹,

Глаголева²,

Galischev³

et al. 2018

Dokl. Akad. nauk

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For the first time, a detailed study of the qualitative and quantitative composition of ginsenosides in the Panax ginseng roots was carried out with the help of high-performance liquid chromatography combined with mass spectrometry (HPLC-MS). The plants were introduced into the conditions of the Republic of Belarus at the experimental plot of the Central Botanical Garden of the National Academy of Sciences of Belarus. It was found that in the examined roots, all basic neutral glycosides of ginseng (ginsenosides Rb1, Rc, Rb2/Rb3, Rd, Rf, Rg1 and Re), as well as their malonylated derivatives (malonylginsenosides Rb1, Rc, Rb2/Rb3, Rd, Rg1 and Re) and some “minor” ginsenosides (20-gluco-ginsenoside Rf, notoginsenosides R1 and R2, isomers of malonyl-ginsenosides Rb1 and Rd) are present. The research also showed that different parts of the P. ginseng roots differ significantly in a total content of ginsenosides: for the main root, this parameter was 3.3 % of dry mass, and for the lateral roots – 7.8 % of dry mass.

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Rare triterpene glycoside of ginseng (ginsenoside malonyl-Rg1) detected in plant cell suspension culture of Panax japonicus var. repens

Cited by 17 publications

References 14 publications

Quality evaluation of Panax ginseng adventitious roots based on ginsenoside constituents, functional genes, and ferric‐reducing antioxidant power

Quality evaluation of Panax ginseng adventitious roots based on ginsenoside constituents, functional genes, and ferric‐reducing antioxidant power

Antihypoxic Action of Panax Japonicus, Tribulus Terrestris and Dioscorea Deltoidea Cell Cultures: In Silico and Animal Studies

Analysis of Gynzenosides in the Roots of Panax Ginseng Introduced in the Central Botanical Garden of Nas of Belarus

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