Ras signaling modulates activity of the ecdysone receptor EcR during cell migration in the drosophila ovary

Hackney, Jennifer F.; Pucci, Christina; Naes, Erin; Dobens, Leonard L.

doi:10.1002/dvdy.21140

Cited by 69 publications

(56 citation statements)

References 76 publications

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“…Because ecdysteroid secretion is mediated through a regulated vesicular trafficking mechanism (42), it is conceivable that follicle cells, nurse cells, or both could produce local pulses of ecdysteroids to promote egg chamber development at each transition during oogenesis. This hypothesis is consistent with the finding that the ligand sensor for ecdysteroids shows a stage-specific activity including stage 10 and stage 14 (32,33). This notion is further supported by this study that up-regulation of Shd in mature follicle cells produces 20E to regulate ovulation and a previous study that local ecdysteroids produced in follicle cells regulate border cell migration (37).…”

Section: Discussionsupporting

confidence: 92%

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Steroid signaling in mature follicles is important for Drosophila ovulation

Knapp

Sun

2017

Proc. Natl. Acad. Sci. U.S.A.

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Although ecdysteroid signaling regulates multiple steps in oogenesis, it is not known whether it regulates Drosophila ovulation, a process involving a matrix metalloproteinase-dependent follicle rupture. In this study, we demonstrated that ecdysteroid signaling is operating in mature follicle cells to control ovulation. Moreover, knocking down shade (shd), encoding the monooxygenase that converts ecdysone (E) to the more active 20-hydroxyecdysone (20E), specifically in mature follicle cells, blocked follicle rupture, which was rescued by ectopic expression of shd or exogenous 20E. In addition, disruption of the Ecdysone receptor (EcR) in mature follicle cells mimicked shd-knockdown defects, which were reversed by ectopic expression of EcR.B2 but not by EcR.A or EcR.B1 isoforms. Furthermore, we showed that ecdysteroid signaling is essential for the proper activation of matrix metalloproteinase 2 (Mmp2) for follicle rupture. Our data strongly suggest that 20E produced in follicle cells before ovulation activates EcR.B2 to prime mature follicles to be responsive to neuronal ovulatory stimuli, thus providing mechanistic insights into steroid signaling in Drosophila ovulation.steroid signaling | ecdysone | ovulation | Shade | EcR isoforms

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Section: Discussionsupporting

confidence: 92%

“…Ecdysteroids have long been known to play essential roles in developmental transitions, such as larval molting and metamorphosis (27,28). In contrast, the roles of ecdysteroids in adult physiology have just started to emerge (29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39).…”

mentioning

confidence: 99%

Steroid signaling in mature follicles is important for Drosophila ovulation

Knapp

Sun

2017

Proc. Natl. Acad. Sci. U.S.A.

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“…Although the in vivo functional significance of USP binding on chorion gene promoters has yet to be investigated, this may be related to a control of the ecdysone signaling on chorion gene expression. Supporting this hypothesis, Hackney et al (2007) have recently shown that the EcR activity regulates chorion gene expression and amplification during eggshell formation.…”

Section: Trans-acting Elementsmentioning

confidence: 81%

Building up the Drosophila eggshell: First of all the eggshell genes must be transcribed

Cavaliere

Bernardi

Romani

et al. 2008

Developmental Dynamics

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The Drosophila eggshell provides a model system for studying the assembly of extracellular matrix. Eggshell formation is a complex process that requires time-coordinated synthesis, cleavage, and transport of various proteins and finally cross-linking mediated by particular functional domains. It has been suggested that the eggshell can act as a storage site for spatial cues involved in embryonic pattern formation. Its structural components are synthesized in the somatic follicle cells in a precise temporally and spatially regulated manner. This review will summarize our knowledge of eggshell gene expression. We will discuss the amplification of the chorion gene clusters and the data acquired on the expression patterns and the regulatory elements controlling transcription of eggshell genes. We will then focus on the findings that correlate follicular epithelium patterning and eggshell gene expression, and discuss the interesting perspectives of an involvement in eggshell assembly of embryonic patterning cues. Developmental Dynamics 237:2061-2072, 2008.

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“…The development and functioning of follicle cells is known to be regulated by ecdysone (Buszczak et al, 1999;Carney and Bender, 2000;Terashima et al, 2005;Hackney et al, 2007;Sun et al, 2008;Bernardi et al, 2009;Boyle and Berg, 2009;Romani et al, 2009). Having characterized SMRTER as a transcriptional Number of eggs produced by the control, homozygous BG01648, and heterozygous BG01648/PL6 females.…”

Section: Smrter Inhibits Ecdysone Activity In Follicle Cellsmentioning

confidence: 99%