Rat aorta as a pharmacological tool for in vitro and in vivo studies

Rameshrad, Maryam; Babaei, Hossein; Azarmi, Yadollah; Fouladi, Daniel Fadaei

doi:10.1016/j.lfs.2015.12.043

Cited by 55 publications

(36 citation statements)

References 226 publications

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“…The pH was 7.38–7.5 at 37°C. Special care was taken to limit endothelial damaging during tissue preparation (Rameshrad et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…Besides, to launch the endothelium integrity, the relaxation response of the segments to cumulative concentration of acetylcholine (10 −9 –10 −4 M) in precontracted rings with phenylephrine (10 −5 M) was assessed. Furthermore, the endothelium‐independent vasorelaxation to cumulative concentration of NO donor sodium nitroprusside (10 −12 –10 −4 M) in precontracted rings with phenylephrine (10 −5 M) was recorded (Rameshrad et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…Isolated rat aortic ring experiments is a golden tool that provides a simple, cheap, and rapid instrument for evaluating the effects of different substances on the vascular function (Rameshrad, Babaei, Azarmi, & Fouladi, ). It should be noticed that endothelial cells have a pivotal role in the vessel health activity by controlling the level of vasoactive compounds and NO.…”

Section: Introductionmentioning

confidence: 99%

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Bisphenol A vascular toxicity: Protective effect of Vitis vinifera (grape) seed extract and resveratrol

Rameshrad

Imenshahidi

Razavi

et al. 2018

Phytotherapy Research

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The underlying mechanism of Bisphenol A (BPA)-induced vascular toxicity and the protective role of grape seed extract (GSE) and resveratrol were investigated in vitro and in vivo. Human umbilical vein endothelial cells (HUVECs) were exposed to different concentrations of GSE and resveratrol. Then, BPA was added to the cells and cell viability and effects on the protein level of cell adhesion molecules were measured through MTT and western blotting. Animals were randomly divided into control, GSE (3 and 12 mg·kg ·day ip), resveratrol (100 mg·kg ·day ip), BPA (35 mg·kg ·day , gavage), BPA plus GSE (3, 6, and 12 mg·kg ·day ip), BPA plus resveratrol (25, 50, and 100 mg·kg ·day ip), and BPA plus vitamin E (200 IU/kg per every other day ip). After 2 months, contractile and relaxant responses were evaluated on the isolated aorta. BPA increased the level of aorta malondialdehyde (p < 0.001) and decreased vascular responses to KCl (p < 0.01), phenylephrine (p < 0.001), and acetylcholine (p < 0.01). In HUVECs, BPA (IC : 220 μM) increased protein level of vascular cell adhesion molecule (p < 0.05) and cleaved capase3 (p < 0.001). GSE, resveratrol, and vitamin E cotreatment restored toxic effects of BPA in some levels. BPA vascular toxicity was attributed to lipid peroxidation and endothelial dysfunction. The protective role of GSE and resveratrol against BPA-endothelial dysfunction could be attributed to their potent antioxidant properties.

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“…The pH was 7.38–7.5 at 37°C. Special care was taken to limit endothelial damaging during tissue preparation (Rameshrad et al, ).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bisphenol A vascular toxicity: Protective effect of Vitis vinifera (grape) seed extract and resveratrol

Rameshrad

Imenshahidi

Razavi

et al. 2018

Phytotherapy Research

Self Cite

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“…To evaluate the vascular function of the endothelium, the relaxation to 10 −6 M acetylcholine (muscarinic agonist) in pre-contracted aortic rings with 10 −6 M PE was tested. According to the general use of rat aorta as a pharmacological tool for in vitro, the aortic rings were considered with a functional endothelial response if relaxation was up to 70% to 80% [49].…”

Section: Vascular Reactivity Experimentsmentioning

confidence: 99%

Fast Isolation of Flavonoids from the Endemic Species Nolana ramosissima I.M. Johnst and Its Endothelium-Independent Relaxation Effect in Rat Aorta

et al. 2020

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The infusion of the desertic plant Nolana ramosissima I.M. Johnst showed vascular smooth muscle relaxation in rat aorta and the presence of several phenolic compounds, which were detected by high resolution UHPLC-Orbitrap-HESI-MS. In addition, five flavonoids were rapidly isolated from a methanolic extract using high-performance counter-current chromatography (HPCCC). The N. ramosissima extract showed endothelium-independent relaxation effect in rat aorta. Sixty-one compounds were detected in the infusion, mainly glycosylated flavonoids, flavanones and several oxylipins, suggesting that a synergistic effect between the compounds in the extracts could be responsible for the relaxation activity. Vascular activity experiments were done in isolated organ bath. In rat aorta, a nitric oxide inhibitor did not prevent the relaxation effects of the extract; however, a selective guanylyl cyclase inhibitor partially blunted this effect. The compound 5,3 -dihydroxy-4 7-dimethoxyflavone presented higher relaxation effect than 100 µg/mL of N. ramosissima extract. The extract and the isolated metabolites from N. ramosissima can show relaxation effects on rat aorta by a mechanism that is independent of the endothelium.

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“…We opted to study the putative interaction in the well-validated [11], and in our previous experiments with lerimazoline already used rat aorta preparation, having in mind the recognized variability of agonist-induced actions in different vascular beds of rats, which depend on concentrations tested, type of receptor involved, basal vascular tone, vascular bed analyzed and the presence of possible pathological processes [121314]. …”

Section: Introductionmentioning

confidence: 99%

Elucidation of the profound antagonism of contractile action of phenylephrine in rat aorta effected by an atypical sympathomimetic decongestant

Rizvić

Janković

Savić

2017

Korean J Physiol Pharmacol

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Vasoconstrictive properties of sympathomimetic drugs are the basis of their widespread use as decongestants and possible source of adverse responses. Insufficiently substantiated practice of combining decongestants in some marketed preparations, such are those containing phenylephrine and lerimazoline, may affect the overall contractile activity, and thus their therapeutic utility. This study aimed to examine the interaction between lerimazoline and phenylephrine in isolated rat aortic rings, and also to assess the substrate of the obtained lerimazoline-induced attenuation of phenylephrine contraction. Namely, while lower concentrations of lerimazoline (10−6 M and especially 10−7 M) expectedly tended to potentiate the phenylephrine-induced contractions, lerimazoline in higher concentrations (10−4 M and above) unexpectedly and profoundly depleted the phenylephrine concentration-response curve. Suppression of NO with NO synthase (NOS) inhibitor Nw-nitro-L-arginine methyl ester (L-NAME; 10−4 M) or NO scavanger OHB12 (10−3 M), as well as non-specific inhibition of K+-channels with tetraethylammonium (TEA; 10−3 M), have reversed lerimazoline-induced relaxation of phenylephrine contractions, while cyclooxygenase inhibitor indomethacin (10−5 M) did not affect the interaction between two vasoconstrictors. At the receptor level, non-selective 5-HT receptor antagonist methiothepin reversed the attenuating effect of lerimazoline on phenylephrine contraction when applied at 3×10−7 and 10−6 M, but not at the highest concentration (10−4 M). Neither the 5-HT1D-receptor selective antagonist BRL 15572 (10−6 M) nor 5-HT7 receptor selective antagonist SB 269970 (10−6 M) affected the lerimazoline-induced attenuation of phenylephrine activity. The mechanism of lerimazoline-induced suppression of phenylephrine contractions may involve potentiation of activity of NO and K+-channels and activation of some methiothepin-sensitive receptors, possibly of the 5-HT2B subtype.

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Rat aorta as a pharmacological tool for in vitro and in vivo studies

Cited by 55 publications

References 226 publications

Bisphenol A vascular toxicity: Protective effect of Vitis vinifera (grape) seed extract and resveratrol

Bisphenol A vascular toxicity: Protective effect of Vitis vinifera (grape) seed extract and resveratrol

Fast Isolation of Flavonoids from the Endemic Species Nolana ramosissima I.M. Johnst and Its Endothelium-Independent Relaxation Effect in Rat Aorta

Elucidation of the profound antagonism of contractile action of phenylephrine in rat aorta effected by an atypical sympathomimetic decongestant

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