Rat model of arterial thrombosis induced by ferric chloride

Kurz, K D; Main, Bradley W.; Sandusky, George E.

doi:10.1016/0049-3848(90)90106-m

Cited by 418 publications

(327 citation statements)

References 6 publications

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“…These observations suggest that the FeCl 3 -induced thrombosis model combined with intravital imaging may provide a better understanding of arterial recanalization and associated tissue perfusion changes after thrombolytic therapy, a subject that has been partly neglected experimentally despite the unsatisfactory success in clinical trials. Kurz et al (1990) first described the use of FeCl 3 to induce arterial thrombosis in rats. Our study shows that this method can be effectively applied to the MCA of small rodents to produce thrombus.…”

Section: Discussionmentioning

confidence: 99%

“…Ferric chloride-induced thrombosis was first reported by Kurz et al (1990) as a small animal model of arterial thrombosis for studying novel antithrombotic agents. In this model, a blood vessel is briefly exposed to FeCl 3 (Denis et al, 1998;Smyth et al, 2001;Weiss et al, 2002), which rapidly results in the formation of a thrombus (Kurz et al, 1990) with a morphology similar to that found in humans (Farrehi et al, 1998). To our knowledge, FeCl 3 has never been applied to the MCA to induce cerebral ischemia, although it was used to produce intracarotid thrombi for studies with antithrombotic agents (Farrehi et al, 1998;Hagedorn et al, 2010;.…”

Section: Introductionmentioning

confidence: 99%

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Thrombotic distal middle cerebral artery occlusion produced by topical FeCl₃ application: A novel model suitable for intravital microscopy and thrombolysis studies

Karataş

Erdener

Gürsoy‐Özdemir

et al. 2011

J Cereb Blood Flow Metab

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Intravital or multiphoton microscopy and laser-speckle imaging have become popular because they allow live monitoring of several processes during cerebral ischemia. Available rodent models have limitations for these experiments; e.g., filament occlusion of the proximal middle cerebral artery (MCA) is difficult to perform under a microscope, whereas distal occlusion methods may damage the MCA and the peri-arterial cortex. We found that placement of a 10% FeCl 3 -soaked filter paper strip (0.3¾1 mm 2 ) on the duramater over the trunk of the distal MCA through a cranial window for 3 minutes induced intraarterial thrombus without damaging the peri-arterial cortex in the mouse. This caused a rapid regional cerebral blood flow decrease within 10 minutes and total occlusion of the MCA segment under the filter paper in 17±2 minutes, which resulted in a typical cortical infarct of 27 ± 4 mm 3 at 24 hours and moderate sensorimotor deficits. There was no significant hemispheric swelling or hemorrhage or mortality at 24 hours. Reperfusion was obtained in half of the mice with tissue plasminogen activator, which allowed live monitoring of clot lysis along with restoration of tissue perfusion and MCA flow. In conclusion, this relatively simple and noninvasive stroke model is easy to perform under a microscope, making it suitable for live imaging and thrombolysis studies.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Thrombotic distal middle cerebral artery occlusion produced by topical FeCl₃ application: A novel model suitable for intravital microscopy and thrombolysis studies

Karataş

Erdener

Gürsoy‐Özdemir

et al. 2011

J Cereb Blood Flow Metab

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“…34 As such, we adapted a ferric chloride (FeCl 3 )-mediated thrombosis technique 35 to produce a complete point occlusion of the dSV. In experiments where a clot was induced, animals were transferred from the imaging facility to the operating room after initial imaging of vascular structure and blood flow speed measurements.…”

Section: Clot Formationmentioning

confidence: 99%

Characterization of Blood Flow in the Mouse Dorsal Spinal Venous System before and after Dorsal Spinal Vein Occlusion

Farrar

Rubin

Diago

et al. 2015

J Cereb Blood Flow Metab

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The availability of transgenic strains has made the laboratory mouse a popular model for the study of healthy and diseased state spinal cord (SC). Essential to identifying physiologic and pathologic events is an understanding of the microvascular network and flow patterns of the SC. Using 2-photon excited fluorescence (2PEF) microscopy we performed in vivo measurements of blood flow in the lower thoracic portion of the mouse dorsal spinal vein (dSV) and in the first upstream branches supplying it, denoted as dorsal ascending venules (dAVs). We found that the dSV had large radiculomedullary veins (RMVs) exiting the SC, and that flow in the dSV between pairs of RMVs was bidirectional. Volumetric flow increased in each direction away from the point of bifurcation. Flow in the upstream dAVs varied with diameter in a manner consistent with a constant distal pressure source. By performing ex vivo 2PEF microscopy of fluorescent-gel perfused tissue, we created a 3-D map of the dorsal spinal vasculature. From these data, we constructed a simple model that predicted changes in the flow of upstream branches after occlusion of the dSV in different locations. Using an atraumatic model of dSV occlusion, we confirmed the predictions of this model in vivo.

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“…Con el propósito de contar con un control positivo atingente al estudio realizado, es que mediante inducción química en arteria de conejo de obtuvo un tejido acorde a la expresión requerida para ICAM-1. Para ello, se utilizó conejo neozelandes, desde el cual, posterior a ser anestesiado, se disecó quirúrgicamente la arteria carótida en la que se indujo la trombosis mediante la aplicación de cloruro férrico 10% (Kurz et al, 1990). Durante el tiempo que duró el procedimiento el animal fue monitoreado mediante Detector de flujo Doppler modelo 811-B (Parks Medical Electronics, USA).…”

Section: Materials Y Métodounclassified

Expresión de ICAM-1 en el Endotelio de Arterias Humanas Mediante Inmunohistoquímica

et al. 2011

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RESUMEN:Las enfermedades cardiovasculares son la principal causa de muerte a nivel mundial. Entre ellas tienen gran relevancia las de tipo isquémicas, en donde el desarrollo de placas ateroscleróticas es el proceso fisiopatológico central. El estudio de la aterosclerosis es fundamental para comprender como se inicia este proceso patológico y los factores que influyen en su desarrollo. Distintas metodologías de laboratorio, entre otras la inmunohistoquímica, permiten reconocer las células y moléculas que participan en el proceso ateromatoso y que van interactuando según la progresión de la lesión. Un marcador de disfunción endotelial es la mayor expresión de la molécula de adhesión intercelular ICAM-1. En este trabajo se realizó la estandarización de inmunohistoquímica para la molécula de adhesión ICAM-1, y se estudió su expresión en arterias humanas sanas y con placa ateromatosa. En las muestras de arterias humanas con patología aterosclerótica, la expresión de ICAM-1 se observó aumentada, pero fue de difícil reconocimiento. Esto principalmente porque el tejido empleado como control en la estandarización fue una amígdala con hiperplasia y proceso inflamatorio que aumenta notablemente la expresión de ICAM-1. La implementación del método de inmunohistoquímica para ICAM-1 en arterias humanas permitirá conocer estados de disfunción endotelial y el desarrollo futuro del diseño e implementación de métodos de diagnóstico en aquellos procesos ateroclerótico en estado incipiente.

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Rat model of arterial thrombosis induced by ferric chloride

Cited by 418 publications

References 6 publications

Thrombotic distal middle cerebral artery occlusion produced by topical FeCl₃ application: A novel model suitable for intravital microscopy and thrombolysis studies

Thrombotic distal middle cerebral artery occlusion produced by topical FeCl₃ application: A novel model suitable for intravital microscopy and thrombolysis studies

Characterization of Blood Flow in the Mouse Dorsal Spinal Venous System before and after Dorsal Spinal Vein Occlusion

Expresión de ICAM-1 en el Endotelio de Arterias Humanas Mediante Inmunohistoquímica

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Rat model of arterial thrombosis induced by ferric chloride

Cited by 418 publications

References 6 publications

Thrombotic distal middle cerebral artery occlusion produced by topical FeCl3 application: A novel model suitable for intravital microscopy and thrombolysis studies

Thrombotic distal middle cerebral artery occlusion produced by topical FeCl3 application: A novel model suitable for intravital microscopy and thrombolysis studies

Characterization of Blood Flow in the Mouse Dorsal Spinal Venous System before and after Dorsal Spinal Vein Occlusion

Expresión de ICAM-1 en el Endotelio de Arterias Humanas Mediante Inmunohistoquímica

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Thrombotic distal middle cerebral artery occlusion produced by topical FeCl₃ application: A novel model suitable for intravital microscopy and thrombolysis studies

Thrombotic distal middle cerebral artery occlusion produced by topical FeCl₃ application: A novel model suitable for intravital microscopy and thrombolysis studies