Rat parietal cells express CCK2 receptor mRNA: gene expression analysis of single cells isolated by laser-assisted microdissection

Tømmerås, Karin; Bakke, Ingrid; Sandvik, Arne K.; Larsson, Erik G.

doi:10.1016/s0006-291x(02)02202-7

Cited by 11 publications

(4 citation statements)

References 29 publications

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“…The cholecystokinin-2 (CCK-2) receptor is a gastrin-specific receptor and is mainly expressed in the gastric parietal cells and enterochromaffin-like (ECL) cells (38)(39)(40). When rats were administered an intravenous injection of gastrin to activate the CCK-2 receptor, heparin-binding epidermal growth factor (HB-EGF) and amphiregulin expression increased, leading to gastric epithelial hyperplasia (41).…”

Section: Glandular Stomachmentioning

confidence: 99%

The effects of chronic bile reflux on the gastric mucosa of rats

Wang

Tang²,

Cao³

2013

Turk J Gastroenterol

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Section: Glandular Stomachmentioning

confidence: 99%

The effects of chronic bile reflux on the gastric mucosa of rats

Wang

Tang²,

Cao³

2013

Turk J Gastroenterol

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“…Morphologically, the cell is identified by the presence of the histamineproducing enzyme histidine decarboxylase ( Figure 4). The ECL cells have muscarinic and b-adrenergic receptors [44], as well as receptors for apelin [54], bradykinin, interleukin-1b, TNF-a, somatostatin and prostaglandin E2 [55], CCK-2 [56], dopamine and gastrin [57], galanin [8], histamine [58], epidermal growth factor (EGF), NGF, and FGF [59], PACAP [60], PYY Y1 [61], TGF-a [62], and VIP [63].…”

Section: Ecl Cellsmentioning

confidence: 99%

The enteroendocrine “letter cells” – time for a new nomenclature?

Helander

Fändriks

2011

Scandinavian Journal of Gastroenterology

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The endocrine cells of the gastrointestinal (GI) tract and the pancreas, referred to as the enteroendocrine cells, secrete a large variety of peptides and amines that regulate functions of the digestive tract itself and of distant organs. Taken together, the enteroendocrine cells form the largest system of endocrine cells in the body, presently comprising 16 cell types. Many of them have been named after letters of the alphabet, but the names are only occasionally related to morphological or functional characteristics of the cell. In this review of the normal, adult, mammalian enteroendocrine cells, we summarize synonyms, functions, locations, structure, stored hormones/amines, receptors, and other cellular expressions. We propose that the enteroendocrine cells should be renamed after their most well-known hormone/amine and, when applicable, their anatomical location, with opportunities for future revisions.

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“…Upon closer examination of the reported cases of multiplex RT-PCR performed using single-cell templates, all but two required a second round of nested PCR using a single set of primers in order to visualize the products [28][29][30]. In other words, co-amplification was performed initially; then, individual aliquots from the first-round of PCR were obtained and each one subjected to single-gene amplification using nested primers.…”

Section: Multiplex Single-cell Rt-pcrmentioning

confidence: 99%

“…Tømmerås et al [28] examined the expression of cholecystokinin-2 receptors, ATPase, and histidine decarboxylase in single rat parietal cells following laser-assisted microdissection, using multiplex RT-PCR. The expression of the tumor necrosis factor receptor [29] and major histocompatibility complex (MHC) class II [30] gene families in single pancreatic b-cells have also been studied using a multiplex format.…”

Section: Introductionmentioning

confidence: 99%

A qualitative look at multiplex gene expression of single cells using capillary electrophoresis

Zabzdyr

Lillard

2005

Electrophoresis

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We demonstrate the first use of capillary electrophoresis with laser-induced fluorescence (CE-LIF) for the qualitative analysis of single-cell multiplex products of the reverse transcriptase-polymerase chain reaction (RT-PCR). The expression of both estrogen receptor alpha (ERalpha) and beta-actin in individual MCF-7 cells was monitored using a one-pot reaction. Reverse transcription and a single round of touch-down PCR, performed in a multiplex format, were used to generate fragment sizes of 318 bp and 838 bp, for ERalpha and beta-actin, respectively. A replaceable hydroxypropylmethylcellulose sieving matrix was used to effect a size-based separation of ethidium bromide-bound DNA. As titration of RT-PCR reaction components did not appreciably influence multiplex product generation, the use of additives, including bovine serum albumin (BSA) and herring sperm DNA, was explored. The addition of BSA to the RT-PCR mixture only resulted in efficient amplification of beta-actin, whereas the DNA carrier allowed co-amplification of both ERalpha and beta-actin. Furthermore, the sensitivity of our CE-LIF method eliminated the need for a second round of nested PCR, typically required when RT-PCR products are analyzed using gel electrophoresis.

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Rat parietal cells express CCK2 receptor mRNA: gene expression analysis of single cells isolated by laser-assisted microdissection

Cited by 11 publications

References 29 publications

The effects of chronic bile reflux on the gastric mucosa of rats

The effects of chronic bile reflux on the gastric mucosa of rats

The enteroendocrine “letter cells” – time for a new nomenclature?

A qualitative look at multiplex gene expression of single cells using capillary electrophoresis

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