Rat retinal vasomotion assessed by laser speckle imaging

Neganova, Anastasiia Y.; Postnov, Dmitry D.; Sosnovtseva, Olga; Jacobsen, Jens Christian Brings

doi:10.1371/journal.pone.0173805

Cited by 13 publications

(8 citation statements)

References 35 publications

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“…Then 3D plot was projected in two dimensions as averaged over time. According to previous reports (Li et al, 2007; Pavlov et al, 2014; Neganova et al, 2017), 0.01–5 Hz was detected and slower contributions (0.01–0.25 Hz) were recognized caused by endothelial factors. The relative value of endothelial factors was recorded as the ratio between the value of endothelial amplitude and the sum amplitudes for total frequency range.…”

Section: Methodsmentioning

confidence: 54%

Exosomes Derived From Pericytes Improve Microcirculation and Protect Blood–Spinal Cord Barrier After Spinal Cord Injury in Mice

Yuan

Wang³

et al. 2019

Front. Neurosci.

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Spinal cord injury (SCI) often leads to severe and permanent paralysis and places a heavy burden on individuals, families, and society. Until now, the therapy of SCI is still a big challenge for the researchers. Transplantation of mesenchymal stem cells (MSCs) is a hot spot for the treatment of SCI, but many problems and risks have not been resolved. Some studies have reported that the therapeutic effect of MSCs on SCI is related to the paracrine secretion of cells. The exosomes secreted by MSCs have therapeutic potential for many diseases. There are abundant pericytes which possess the characteristics of stem cells in the neurovascular unit. Due to the close relationship between pericytes and endothelial cells, the exosomes of pericytes can be taken up by endothelial cells more easily. There are fewer studies about the therapeutic potential of the exosomes derived from pericytes on SCI now. In this study, exosomes of pericytes were transplanted into the mice with SCI to study the restoration of motor function and explore the underlying mechanism. We found that the exosomes derived from pericytes could reduce pathological changes, improve the motor function, the blood flow and oxygen deficiency after SCI. In addition, the exosomes could improve the endothelial ability to regulate blood flow, protect the blood-spinal cord barrier, reduce edema, decrease the expression of HIF-1α, Bax, Aquaporin-4, and MMP2, increase the expression of Claudin-5, bcl-2 and inhibit apoptosis. The experiments in vitro proved that exosomes derived from pericytes could protect the barrier of spinal cord microvascular endothelial cells under hypoxia condition, which was related to PTEN/AKT pathway. In summary, our study showed that exosomes of pericytes had therapeutic prospects for SCI.

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Section: Methodsmentioning

confidence: 54%

Exosomes Derived From Pericytes Improve Microcirculation and Protect Blood–Spinal Cord Barrier After Spinal Cord Injury in Mice

Yuan

Wang³

et al. 2019

Front. Neurosci.

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“…Despite obvious advantages and active research, only a few studies on human skin microcirculation conducted with the LSCI method have real clinical significance. Most of the works on LSCI image processing are devoted to theoretical issues of processing raw speckle images [25], [26] or cerebral applications [27], [28], whereas the linear and nonlinear analysis of the main microvascular regulatory mechanisms developed for the LDF method could also benefit LSCI signal studies. This has been confirmed in [29] using an empirical mode decomposition analysis of LSCI and LDF signals variability.…”

Section: Introductionmentioning

confidence: 99%

Wavelet Analysis of the Temporal Dynamics of the Laser Speckle Contrast in Human Skin

Mizeva

Dremin

Potapova

et al. 2019

IEEE Trans. Biomed. Eng.

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Objective: Spectral analysis of laser Doppler flowmetry (LDF) signals has been widely used in studies of physiological vascular function regulation. An alternative to LDF is the laser speckle contrast imaging method (LSCI), which is based on the same physical principle. In contrast to LDF, LSCI provides non-scanning full-field imaging of a relatively wide skin area and offers high spatial and temporal resolutions, which allows visualization of microvascular structure. This circumstance, together with a large number of works which had shown the effectiveness of temporal LSCI analysis, gave impetus to experimental studies of the relation between LDF and LSCI used to monitor the temporal dynamics of blood flow. Methods: Continuous wavelet transform was applied to construct a timefrequency representation of a signal. Results: Analysis of 10 minute LDF and LSCI output signals recorded simultaneously revealed rather high correlation between oscillating components. It was demonstrated for the first time that the spectral energy of oscillations in the 0.01-2 Hz frequency range of temporal LSCI recordings carries the same information as the conventional LDF recordings and hence it reflects the same physiological vascular tone regulation mechanisms. Conclusion: The approach proposed can be used to investigate speckle pattern dynamics by LSCI in both normal and pathological conditions. Significance: The results of research on the influence of spatial binning and averaging on the spectral characteristics of perfusion monitored by LSCI are of considerable interest for the development of LSCI systems optimized to evaluate temporal dynamics.

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“…This makes it possible to study vasomotion over larger areas and to investigate spatial and temporal synchronicity, which is hard to accomplish using LDF. To our knowledge, LSCI has only been used to study vasomotion in the retina of the mouse [32].…”

Section: Introductionmentioning

confidence: 99%

The presence of synchronized perfusion dips in the microcirculation of the resting nail bed

Mirdell

Lemstra-Idsardi

Farnebo

et al. 2019

Microvascular Research

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Objectives: Laser speckle contrast imaging (LSCI) has seen limited use in the study of perfusion dynamics such as vasomotion. The aim of this study was to investigate the effects of a prolonged seated position on perfusion dynamics in the nail bed using LSCI. Methods: Perfusion was recorded in digits II to IV bilaterally for 20 min during two separate sessions in ten healthy volunteers. The acclimatization period was 5 min for the 1 st session and 20 min for the 2 nd. Perfusion variability and the presence of recurring perfusion dips were analyzed. A digital nerve block was done to verify suspected nervous origin of phenomenon. Results: Synchronized phases of vasoconstriction were observed in all subjects with perfusion dips in all digits bilaterally and simultaneously. Application of a digital nerve block abolished perfusion dips. The frequency of this phenomenon increased by 25.0% (95% CI: 1.6 to 49.2%) in the left-hand digits after a prolonged seated position. Perfusion variability increased by 11.6% (95% CI: 2.6 to 20.3%) in the digits of the left hand. Perfusion changes in right-hand digits did not significantly increase. During the 1 st session, temperature increased by 2.7°C (1.1 to 4.2) while it decreased by 1.3°C (0.2 to 2.4) during the 2 nd session. Conclusion: The observed perfusion dips are of a centrally mediated nervous origin but are also affected by local factors. They are affected by seating duration and differ between left and right hands, likely because of local micro perfusion dips. This phenomenon seems related to digital thermoregulation.

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Rat retinal vasomotion assessed by laser speckle imaging

Cited by 13 publications

References 35 publications

Exosomes Derived From Pericytes Improve Microcirculation and Protect Blood–Spinal Cord Barrier After Spinal Cord Injury in Mice

Exosomes Derived From Pericytes Improve Microcirculation and Protect Blood–Spinal Cord Barrier After Spinal Cord Injury in Mice

Wavelet Analysis of the Temporal Dynamics of the Laser Speckle Contrast in Human Skin

The presence of synchronized perfusion dips in the microcirculation of the resting nail bed

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