Upstream time-dependent wavelength drift of Directly Modulated Lasers (DMLs) under burst-mode operation is measured on a Second Next Generation Passive Optical Network (NG-PON2) pre-commercial system. A new measurement method is used to demonstrate that the ITU-T (International Telecommunication Union) recommendations on maximum wavelength drift excursion should be improved. Considering the proximity of the different wavelength channels in the ITU-T grid (down to 50GHz), the time-dependent wavelength drift of directly modulated optical emitters is shown to lead to communication degradation and may cause breakdowns. A way to mitigate this problem is proposed. Index Terms-Adiabatic chirp, direct modulation, heterodyne detection, second next generation passive optical network (NG-PON2), thermal chirp, time-dependent wavelength drift, time and wavelength division multiplexing (TWDM), time division multiplexing (TDM), wavelength division multiplexing (WDM).