This study purpose was to analyze the effect of EIMD in gross efficiency and in aerobic and anaerobic cycling performance. Nine well-trained cyclists (30.8 ± 6.4 years, cycling experience 8.4 ± 5.6 years) visited the laboratory 5 times. During the first visit, they performed a maximal incremental test on a cycle ergometer, to identify VO2max (55.2 ± 4.9 mL.kg.min) and maximum aerobic power (Pmax; 327.0 ± 28.5 watts). During the second visit (control), they cycled 5 minutes at 60% of Pmax, 5 minutes at 70% of Pmax, 5 minutes time-trial, and Wingate test. During the third visit, the athletes performed 10 sets of 10 counter-movement jumps, to generate EIMD. The athletes repeated the second visit tests (control) 30 minutes, 48h (fourth visit) and 96h (fifth visit) after the jumps. The RPE values increased 48h after EIMD (3.8 vs. 3.1) at 60% of Pmax. The ventilation and RER increased at 60% of Pmax (up to 4.3 L.min and 0.04, respectively) and at 70% of Pmax (up to 5.4 L.min and 0.05, respectively), mainly after 96h. There was no significant difference in VO2, VCO2, and HR in sub-maximal exercises, neither in time-trial. No differences were observed in the Wingate tests. In conclusion, the EIMD didn't impair gross efficiency, nor aerobic and anaerobic performances in trained cyclists. However, despite the benefits of strength training to improve cyclists' performance, coaches must be cautious to the days after the strength training sessions, because EIMD may change the perception of maintaining a given submaximal intensity during training or competition.