Rate of increase of osmolality determines osmotic  tolerance of mouse inner medullary epithelial cells

Cai, Qing-Qing; Michea, Luis; Andrews, Peter M.; Zhang, Zheng; Rocha, Gerson; Dmitrieva, Natalia I.; Burg, Maurice B.

doi:10.1152/ajprenal.00046.2002

Cited by 29 publications

(30 citation statements)

References 29 publications

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“…In these experiments, cells were isolated from adult animals and kept in culture under numerous passages. Experimental exposure was performed by adding NaCl and urea to the culture medium until 1640 mosmol/kg H 2 O was reached (27). During this treatment, only 30% of cells survived for 24 h. However, when the exposure to urea was made in a linear increase over 20 h, nearly 90% of the cells stayed viable 24 h later.…”

Section: Discussionmentioning

confidence: 99%

Urea Restrains Aldosterone-Induced Development of Peanut Agglutinin–Binding on Embryonic Renal Collecting Duct Epithelia

Schumacher¹,

Strehl²,

Minuth³

2003

Journal of the American Society of Nephrology

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Abstract. Peanut agglutinin (PNA) represents a commonly used marker for ␤-type intercalated (IC) cells and their distribution in the corticomedullary course of the collecting duct (CD) in the mature rabbit kidney. It has been shown that aldosterone is able to generate Ͼ90% of PNA-binding cells in an embryonic CD epithelium in vitro. In adult kidney, a maximum of only 25% PNA-positive cells is found in the cortical segment of the CD, and PNA-binding completely disappears in the inner-medullary CD. Molecules that regulate the gradual development of CD-specific cells during organ growth are unknown. In the present experiments, it was found that addition of physiologic concentrations of urea to the culture medium is able to restrain the action of aldosterone in embryonic CD epithelia. Urea antagonizes in a concentration-dependent manner the action of aldosterone finally leading to only 10% of PNA-binding cells. The data point to a urea-specific effect, because osmolytes such as NaCl and mannitol did not affect PNA binding. In addition, urea did not influence expression of principal-cell typical markers such as AQP2 and 3. The findings may explain that a higher number of PNA-positive cells is found in the cortical region of the kidney correlated with a low concentration of urea as compared with only few PNA-binding cells in the medullary CD, where a high concentration of urea occurs. Thus, an increasing concentration of urea may trigger the number of PNA-positive cells in the cortical-medullary course of the CD during organ development.

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Section: Discussionmentioning

confidence: 99%

Urea Restrains Aldosterone-Induced Development of Peanut Agglutinin–Binding on Embryonic Renal Collecting Duct Epithelia

Schumacher¹,

Strehl²,

Minuth³

2003

Journal of the American Society of Nephrology

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“…For experiments, cells were plated in the same medium on 8-well slides (Nalge Nunc International, Naperville, IL) or 26-mm cell culture inserts (Becton-Dickinson Labware, Bedford, MA) for 3 days, then in the same medium without serum for 48 h before experimental change in osmolality. Osmolality was increased by increasing NaCl or urea concentration either in a single step or linearly over 19 h (5).…”

Section: Methodsmentioning

confidence: 99%

Effects of expression of p53 and Gadd45 on osmotic tolerance of renal inner medullary cells

Cai¹,

Dmitrieva²,

Ferraris³

et al. 2006

American Journal of Physiology-Renal Physiology

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The response of renal inner medullary (IM) collecting duct cells (mIMCD3) to high NaCl involves increased expression of Gadd45 and p53, both of which have important effects on growth and survival of the cells. However, mIMCD3 cells, being immortalized by SV40, proliferate rapidly, which is known to sensitize cells to high NaCl, whereas IM cells in situ proliferate very slowly and survive much higher levels of NaCl. In the present studies, we have examined the importance of Gadd45 and p53 for survival of normal IM cells in their usual high-NaCl environment by using more slowly proliferating second-passage mouse inner medullary epithelial (p2mIME) cells and comparing cells from wild-type and gene knockout mice. Acutely elevating NaCl (and/or urea) reduces Gadd45a, but increases Gadd45b and Gadd45g mRNA, depending on the mix of NaCl and urea and the rate of increase of osmolality. Nevertheless, p2mIME cells from Gadd45b(-/-), Gadd45g(-/-), and Gadd45bg(-/-) mice survive elevation of NaCl (or urea) essentially the same as do wild-type cells. p53(-/-) Cells do not tolerate as high a concentration of NaCl (or urea) as p53(+/+) cells, but urinary concentrating ability of p53(-/-) mice is normal, as is the histology of inner medullas from p53(-/-) and Gadd45abg(-/-) mice. Thus although Gadd45 and p53 may play roles in osmotically stressed mIMCD3 cells, we do not find that their expression makes an important difference, either for Gadd45 in slower proliferating p2mIME cells or for Gadd45 or p53 in normal inner medullary epithelial cells in situ.

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“…Some types of cell are exposed to a broad range of osmotic pressure fluctuations in their natural environment and are resistant to medium hypertonicity [1][2][3][4]. Strongly hypertonic solutions have often been used recently in the medical treatment of patient injuries [5][6][7] and to modify gene expression and enzyme activity [8,9].…”

Section: Introductionmentioning

confidence: 99%

Solute-dependent activation of cell motility in strongly hypertonic solutions in Dictyostelium discoideum, human melanoma HTB-140 cells and walker 256 carcinosarcoma cells

Korohoda

Kucia

Wybieralska

et al. 2011

Cellular and Molecular Biology Letters

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Published data concerning the effects of hypertonicity on cell motility have often been controversial. The interpretation of results often rests on the premise that cell responses result from cell dehydration, i.e. osmotic effects. The results of induced hypertonicity on cell movement of Dictyostelium discoideum amoebae and human melanoma HTB-140 cells reported here show that: i) hypertonic solutions of identical osmolarity will either inhibit or stimulate cell movement depending on specific solutes (Na+ or K+, sorbitol or saccharose); ii) inhibition of cell motility by hypertonic solutions containing Na+ ions or carbohydrates can be reversed by the addition of calcium ions; iii) various cell types react differently to the same solutions, and iv) cells can adapt to hypertonic solutions. Various hypertonic solutions are now broadly used in medicine and to study modulation of gene expression. The observations reported suggest the need to examine whether the other responses of cells to hypertonicity can also be based on the solute-dependent cell responses besides cell dehydration due to the osmotic effects.

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Rate of increase of osmolality determines osmotic tolerance of mouse inner medullary epithelial cells

Cited by 29 publications

References 29 publications

Urea Restrains Aldosterone-Induced Development of Peanut Agglutinin–Binding on Embryonic Renal Collecting Duct Epithelia

Urea Restrains Aldosterone-Induced Development of Peanut Agglutinin–Binding on Embryonic Renal Collecting Duct Epithelia

Effects of expression of p53 and Gadd45 on osmotic tolerance of renal inner medullary cells

Solute-dependent activation of cell motility in strongly hypertonic solutions in Dictyostelium discoideum, human melanoma HTB-140 cells and walker 256 carcinosarcoma cells

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