Solute-dependent activation of cell motility in strongly hypertonic solutions in Dictyostelium discoideum, human melanoma HTB-140 cells and walker 256 carcinosarcoma cells

Korohoda, W; Kucia, Magda; Wybieralska, Ewa; Wianecka-Skoczeń, Magdalena; Waligórska, Agnieszka; Drukała, Justyna; Madeja, Zbigniew

doi:10.2478/s11658-011-0015-y

Cited by 5 publications

(4 citation statements)

References 46 publications

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“…These results show that calcineurin plays a role in cation homeostasis during development in D. discoideum . It has also been shown in other organisms that calcineurin is involved in cation homeostasis , and for D. discoideum the influence of cations on development has been investigated . However, the developmental aspect of cation homeostasis in connection with calcineurin has not been investigated so far.…”

Section: Resultsmentioning

confidence: 99%

Stress and development in Dictyostelium discoideum: the involvement of the catalytic calcineurin A subunit

Thewes

Schubert

Park

et al. 2013

J. Basic Microbiol.

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Calcium signaling is one of the most important signaling-pathways in all eukaryotes. One important target activated by an increased intracellular calcium concentration via calmodulin is the protein phosphatase calcineurin, which is composed of a catalytic subunit (calcineurin A) and a regulatory subunit (calcineurin B). The importance of calcium and calcineurin for the differentiation and development of the social amoeba Dictyostelium discoideum has already been shown by pharmacological approaches. However, so far only a RNAi-silenced calcineurin B mutant has been investigated on a molecular level. Here, we describe the construction and phenotypic investigation of a RNAi-silenced calcineurin A mutant. Phenotypic aberrations during development resemble those produced by silencing of calcineurin B with ectopic tip formation of the fruiting bodies. Additionally, we tested the response of the mutants under various stress conditions in liquid culture as well as during development. Both, calcineurin A and B RNAi-mutants, are hypersensitive during development towards cation stress. Besides its role in development, calcineurin is thus also involved in the stress response in D. discoideum. Further, our data imply that many functions of calcineurin are conserved among the eukaryotes.

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Section: Resultsmentioning

confidence: 99%

Stress and development in Dictyostelium discoideum: the involvement of the catalytic calcineurin A subunit

Thewes

Schubert

Park

et al. 2013

J. Basic Microbiol.

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“…First, after [32], the permeability of the lens cell membrane for Ca 2+ should be in the range (8 x 10 -15 cm s -1 , 2.5 x 10 -11 cm s -1 ). Rescaling this to the conductance values for equation (2) results in a g Ca contained in the range (132 pS m -2 , 412.4 nS m -2 ). On the other hand, based on [33], one can expect leak calcium currents of magnitude equal to 0.36 pA in A7r5 cells that have a diameter of about 16 μm.…”

Section: Methodsmentioning

confidence: 99%

“…Among other things, they are essential for wound healing, organ and tissue formation in morphogenesis, the immunological response, and sperm cell and bacterial movement [1,2]. Galvanotaxis is a special class of the motility processes which is guided by external electric fields [3,4], unlike the other common types of guided migration, such as chemotaxis, which is driven by chemical compound gradients [5] or phototaxis, which is driven by light intensity [6].…”

Section: Introductionmentioning

confidence: 99%

The role of passive calcium influx through the cell membrane in galvanotaxis

Borys

2013

Cellular and Molecular Biology Letters

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Passive calcium influx is one of the theories to explain the cathodal galvanotaxis of cells that utilize the electric field to guide their motion. When exposed to an electric field, the intracellular fluid becomes polarized, leading to positive charge accumulation on the cathodal side and negative charge accumulation on the anodal side. The negative charge on the anodal side attracts extracellular calcium ions, increasing the anodal calcium concentration, which is supposed to decrease the mobile properties of this side. Unfortunately, this model does not capture the Ca 2+ dynamics after its presentation to the intracellular fluid. The ions cannot permanently accumulate on the anodal side because that would build a potential drop across the cytoplasm leading to an ionic current, which would carry positive ions (not only Ca 2+ ) from the anodal to the cathodal part through the cytoplasm. If the cytoplasmic conductance for Ca 2+ is low enough compared to the membrane conductance, the theory could correctly predict the actual behavior. If the ions move through the cytoplasm at a faster rate, compensating for the passive influx, this theory may fail. This paper contains a discussion of the regimes of validity for this theory.

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“…The interpretation of obtained data often rests mainly on the observed cell dehydration due to occurred osmotic shock (Saheb, Biton, Maringer, & Bush, 2013), but not on the quantitative assessment of the overall energy metabolism alteration. Thus, the published reports especially about cell motility and adaptation in hypertonic environment are often controversial (Korohoda et al, 2011), which is problematic since they play a pivotal role in metastasis research, embryogenesis, and so forth. (Yamaguchi et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Two‐photon microscopy assessment of the overall energy metabolism alteration of amoeba in hypertonic environment

Loukanov

2019

Microscopy Res & Technique

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In this study, a two‐photon fluorescence microscopic imaging technique is reported for assessment the effect of dynamic hypertonic environment on the overall energy metabolism alteration and adaptation of soil‐living amoeba Dictyostelium discoideum. For that purpose the fluorescence intensity of mitochondrial reduced nicotinamide adenine dinucleotide (NADH) was monitored and quantified in order to evaluate the corresponded metabolic state of monolayer cultured cells. The two‐photon excitation of NADH with 720 nm near infrared irradiation produced blue fluorescence emission with maximum wavelength centered at 460 nm. The benefits of reported noninvasive microscopic technique are the significantly less cellular damage and avoiding the excitation of other biomolecules except of NADH. It enabled to acquire data for NADH levels of the observed cells on agar plate specimen and hypertonic nutrition media in a Petri dish. The method demonstrated also good sensitivity, reproducibility and the obtained results revealed that D. discoideum species form aggregation in hypertonic environment within several minutes with aim to survive. The formed aggregate had amorphous shape and it consisted from dozen amoeba cells, which kept their NADH amount in constant level for few hours. The reported imaging method might be applicable in various studies for characterization of metabolic events and assessment of the cell energy balance in hypertonic environment.

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Solute-dependent activation of cell motility in strongly hypertonic solutions in Dictyostelium discoideum, human melanoma HTB-140 cells and walker 256 carcinosarcoma cells

Cited by 5 publications

References 46 publications

Stress and development in Dictyostelium discoideum: the involvement of the catalytic calcineurin A subunit

Stress and development in Dictyostelium discoideum: the involvement of the catalytic calcineurin A subunit

The role of passive calcium influx through the cell membrane in galvanotaxis

Two‐photon microscopy assessment of the overall energy metabolism alteration of amoeba in hypertonic environment

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