For the orthogonal time frequency space (OTFS) modulation, we generally multiplex symbols on a new type of carrier waveform in the delay-Doppler (DD) domain. These two parameters can be used to infer the range (R) and velocity (V) of the communication user and sensing target; thus, it is natural for the OTFS to be implemented in integrated sensing and communication (ISAC). A framework for ISAC based on OTFS modulation is proposed in this paper, in which the matched filter scheme with fractional parameter estimation is implemented for radar sensing. In addition, the hybrid message passing (MP) detection algorithm is developed for OTFS symbol demodulation. According to the simulation results, fractional DD shifts associated with multiple targets can be accurately obtained through the proposed framework. Meanwhile, the bit error rate under the proposed detector is less than 10−4 when the signal-to-noise ratio is high enough.