Rates of attachment of fibroblasts to self-assembled monolayers formed by the adsorption of alkylthiols onto gold surfaces

Cooper, Elaine; Wiggs, Robin; Hutt, David A.; Parker, Lesley; Leggett, Graham; Parker, Terry L.

doi:10.1039/a607204f

Cited by 59 publications

(66 citation statements)

References 34 publications

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“…This finding supports previous studies reporting the inability of cells to adhere to methyl-terminated SAMs in the presence of serum [36][37][38]45]. Selective cellular adhesion suggests that hydrophilic/hydrophobic patterning strategies not only aid in the placement of complexes [32,42,43] but also the attachment of cells [36][37][38], resulting in patterns of transfection. In a similar study, micropatterned SAMs greatly facilitated regionally defined loading of DNAs and their expression in mammalian cells [32], however cellular adhesion was not patterned, presumably due to choice of cell type.…”

Section: Patterned Transfectionsupporting

confidence: 90%

“…2B). Low transfection on the DT10 substrates could be due to a combination of factors, including insufficient cell adhesion [37] or complex conformation changes induced upon binding, which have been shown to contribute to irreversible binding of proteins to hydrophobic surfaces [39]. Total protein amount on the hydrophobic surfaces, as determined by the BCA assay, was similar or greater than all other surfaces, indicating that low transfection on these substrates was not due to insufficient cell numbers (data not shown).…”

Section: Transfection On Bulk Sams On Goldmentioning

confidence: 89%

“…SAMs were explored to examine the relationship between surface chemistry and substrate-mediated transfection. SAMs of alkanethiols on gold can be used in cell culture for periods of days [25] and have been used in many cell culture studies, particularly to understand molecular surface determinants required for adhesion dependent cell growth and proliferation [36][37][38].…”

Section: Transfection On Bulk Sams On Goldmentioning

confidence: 99%

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Substrate-mediated delivery from self-assembled monolayers: Effect of surface ionization, hydrophilicity, and patterning

2005

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Gene transfer has many potential applications in basic and applied sciences. In vitro, DNA delivery can be enhanced by increasing the concentration of DNA in the cellular microenvironment through immobilization of DNA to a substrate that supports cell adhesion. Substrate-mediated delivery describes the immobilization of DNA, complexed with cationic lipids or polymers, to a biomaterial or substrate. As surface properties are critical to the efficiency of the surface delivery approach, selfassembled monolayers (SAMs) of alkanethiols on gold were used to correlate surface chemistry of the substrate to binding, release, and transfection of non-specifically immobilized complexes. Surface hydrophobicity and ionization were found to mediate both DNA complex immobilization and transfection, but had no effect on complex release. Additionally, SAMs were used in conjunction with soft lithographic techniques to imprint substrates with specific patterns, resulting in patterned DNA complex deposition and transfection, with transfection efficiencies in the patterns nearing 40%. Controlling the interactions between complexes and substrates, with the potential for patterned delivery, can be used to locally enhance or regulate gene transfer, with applications to tissue engineering scaffolds and transfected cell arrays.

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Section: Patterned Transfectionsupporting

confidence: 90%

Section: Transfection On Bulk Sams On Goldmentioning

confidence: 89%

Section: Transfection On Bulk Sams On Goldmentioning

confidence: 99%

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Substrate-mediated delivery from self-assembled monolayers: Effect of surface ionization, hydrophilicity, and patterning

2005

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“…A monolayer containing no EG thiols resulted in a robust cell adhesion (Fig. 1a), similar to previous results on COO − -terminated SAMs [41,42]. Increasing the percentage of EG groups to 20 and 40% (Fig.…”

Section: Cell Adhesion On Eg Samssupporting

confidence: 90%

Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes

2008

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Substrate-mediated gene delivery describes the immobilization of gene therapy vectors to a biomaterial, which enhances gene transfer by exposing adhered cells to elevated DNA concentrations within the local microenvironment. Surface chemistry has been shown to affect transfection by nonspecifically immobilized complexes using self-assembled monolayers (SAMs) of alkanethiols on gold. In this report, SAMs were again used to provide a controlled surface to investigate whether the presence of oligo(ethylene glycol) (EG) groups in a SAM could affect complex morphology and enhance transfection. EG groups were included at percentages that did not affect cell adhesion. Nonspecific complex immobilization to SAMs containing combinations of EG-and carboxylic acidterminated alkanethiols resulted in substantially greater transfection than surfaces containing no EG groups or SAMs composed of EG groups combined with other functional groups. Enhancement in transfection levels could not be attributed to complex binding densities or release profiles. Atomic force microscopy imaging of immobilized complexes revealed that EG groups within SAMs affected complex size and appearance and could indicate the ability of these surfaces to preserve complex morphology upon binding. The ability to control the morphology of the immobilized complexes and influence transfection levels through surface chemistry could be translated to scaffolds for gene delivery in tissue engineering and diagnostic applications.

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“…The design of surfaces to elicit specific cellular responses has, accordingly, been delayed. In this regard, SAMs have the capability to produce well-defined surfaces with known structures and properties that may be carefully regulated and manipulated [6,[13][14][15][16]. Cell attachment may also be influenced by the increased absorption of fibronectin on SAMs [17] or binding of the protein in an orientation such that cell binding sites are accessible [18].…”

Section: Introductionmentioning

confidence: 99%

Growth and Functionality of Cells Cultured on Conducting and Semi-Conducting Surfaces Modified with Self-Assembled Monolayers (SAMs)

et al. 2016

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Abstract:Bioengineering of dermal and epidermal cells on surface modified substrates is an active area of research. The cytotoxicity, maintenance of cell phenotype and long-term functionality of human dermal fibroblast (HDF) cells on conducting indium tin oxide (ITO) and semi-conducting, silicon (Si) and gallium arsenide (GaAs), surfaces modified with self-assembled monolayers (SAMs) containing amino (-NH 2 ) and methyl (-CH 3 ) end groups have been investigated. Contact angle measurements and infrared spectroscopic studies show that the monolayers are conformal and preserve their functional end groups. Morphological analyses indicate that HDFs grow well on all substrates except GaAs, exhibiting their normal spindle-shaped morphology and exhibit no visible signs of stress or cytoplasmic vacuolation. Cell viability analyses indicate little cell death after one week in culture on all substrates except GaAs, where cells died within 6 h. Cells on all surfaces proliferate except on GaAs and GaAs-ODT. Cell growth is observed to be greater on SAM modified ITO and Si-substrates. Preservation of cellular phenotype assessed through type I collagen immunostaining and positive staining of HDF cells were observed on all modified surfaces except that on GaAs. These results suggest that conducting and semi-conducting SAM-modified surfaces support HDF growth and functionality and represent a promising area of bioengineering research.

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Rates of attachment of fibroblasts to self-assembled monolayers formed by the adsorption of alkylthiols onto gold surfaces

Cited by 59 publications

References 34 publications

Substrate-mediated delivery from self-assembled monolayers: Effect of surface ionization, hydrophilicity, and patterning

Substrate-mediated delivery from self-assembled monolayers: Effect of surface ionization, hydrophilicity, and patterning

Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes

Growth and Functionality of Cells Cultured on Conducting and Semi-Conducting Surfaces Modified with Self-Assembled Monolayers (SAMs)

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