Rates of respiration in the light measured in marine phytoplankton using an 18O isotope-labelling technique

Grande, Karen D.; Marra, John; Langdon, C.; Heinemann, Kristina; Bender, Michael L.

doi:10.1016/0022-0981(89)90050-6

Cited by 86 publications

(62 citation statements)

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“…The Chl-specific respiration rates measured here ranged to much higher values than those reported for single-species cultures (Stone and Ganf 198 1;Grande et al 1989;Weger et al 1989), indicating substantial nonalgal respiration. The contributions of other pond microbes and zooplankton were not assessed separately.…”

Section: Discussioncontrasting

confidence: 56%

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Diel cycles of planktonic respiration rates in briefly incubated water samples from a fertile earthen pond

et al. 1992

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Planktonic community respiration rates were assessed every 30 min through two 48-h periods in near-surface water taken automatically from a fertilized carthen pond and incubated in a plastic chamber for 2 1 min of each sampling cycle. Parallel records of water temperature, air temperature, windspccd, and solar irradiance permitted calculation of gross and net primary production and photosynthesis-irradiance relationships. Nighttime respiration rates generally matched oxygen depletion rates in pond water, indicating that incubation-based rates were representative of a quickly darkened pond community throughout the day. Daytime rates averaged nearly 2 times the mean night rate and 58% higher than the mean day rate determined by a typical interpolation used in free-water production calculations. Daily gross production ranged from 0.7 to 1.2 mmol 0, liter-l d I; respiration constituted 65-75% of gross rates. Gross oxygen production per unit Chl a during sampling intervals was light saturated at irradiance values >600 PEinst m-2 s-l, with an asymptotic value of 1.58 pmol 0, (kg Chl a) -I h I. This system and method were capable ofresolving respiration and gross and net production when chlorophyll concentrations were near 40 pg liter-l.

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Section: Discussioncontrasting

confidence: 56%

“…This technique is able to detect only the mitochondrial component of light respiration, which has been shown to be subject to light enhancement by Grande et al (1989) and Weger et al (1989). Photorespiration effects, which were not detected in those isotope-based studies of single-species cultures, would not be seen in our dark chamber.…”

Section: Discussionmentioning

confidence: 91%

Diel cycles of planktonic respiration rates in briefly incubated water samples from a fertile earthen pond

et al. 1992

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“…The NCP fluxes after the pCO 2 shift in the 2 chemostats were significantly different, essentially due to the decrease in DCR fluxes in the LH chemostat. The average ratio of GCP/DCR, an index of the net efficiency of the cells to fix carbon into the organic pool, was 3.1 ± 0.7, which is higher than previously reported for batch cultures (1.6: Grande et al 1989, and2.6: Eppley &Sloan 1965). This ratio indicates that nearly 68% of the inorganic carbon fixed by the cell through photosynthesis was converted into organic biomass, the remaining 32% being released into the inorganic carbon pool.…”

Section: Net Primary Production and Respirationcontrasting

confidence: 55%

Response of coccolithophorid Emiliania huxleyi to elevated partial pressure of CO2 under nitrogen limitation

Sciandra¹,

Harlay²,

Lefèvre³

et al. 2003

Mar. Ecol. Prog. Ser.

176

152

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Precipitation of calcium carbonate by phytoplankton in the photic oceanic layer is an important process regulating the carbon cycling and the exchange of CO 2 at the ocean-atmosphere interface. Previous experiments have demonstrated that, under nutrient-sufficient conditions, doubling the partial pressure of CO 2 (pCO 2 ) in seawater -a likely scenario for the end of the centurycan significantly decrease both the rate of calcification by coccolithophorids and the ratio of inorganic to organic carbon production. The present work investigates the effects of high pCO 2 on calcification by the coccolithophore Emiliania huxleyi (Strain TW1) grown under nitrogen-limiting conditions, a situation that can also prevail in the ocean. Nitrogen limitation was achieved in NO 3 -limited continuous cultures renewed at the rate of 0.5 d -1 and exposed to a saturating light level. pCO 2 was increased from 400 to 700 ppm and controlled by bubbling CO 2 -rich or CO 2 -free air into the cultures. The pCO 2 shift has a rapid effect on cell physiology that occurs within 2 cell divisions subsequent to the perturbation. Net calcification rate (C ) decreased by 25% and, in contrast to previous studies with N-replete cultures, gross community production (GCP) and dark community respiration (DCR) also decreased. These results suggest that increasing pCO 2 has no noticeable effect on the calcification/photosynthesis ratio (C /P) when cells of E. huxleyi are NO 3 -limited.

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“…Not including this probability in the calculation of gross oxygen production, as was the case in this study, may result in consistent underestimation of gross oxygen production rates and consequently of a(),. Experimental evidence for increases in mitochondrial respiration rate in the light has been found for a number of Chl ccontaining algae (Grande et al 1989;Weger et al 1989;Daneri et al 1992;Beardall et al 1994). The higher mitochondrial respiration rates in the light are probably due to increased substrate supply by photosynthesis.…”

Section: Discussionmentioning

confidence: 94%

Light dependence of quantum yields for PSII charge separation and oxygen evolution in eucaryotic algae

Flameling

Kromkamp

1998

Limnology & Oceanography

126

105

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Quantum yields of photosystcm I1 (PSII) charge separation (@& and oxygen production ((I) (,,) were determined by simultaneous measurements of oxygen production and variable fluorescence in four different aquatic microalgae representing three different taxonomic groups: the freshwater alga Scenedesmus protuberans (Chlorophyceae) and the marine algae Phneorystis globosa (Prymnesiophyceae), Emiliania huxleyi (Prymnesiophyceae), and Phaeodactylum tricornutum (Bacillariophyceae). In S. protuberans, P. tricornutum, and E. huxleyi, light-dependent variability was observed in the ratio of a(,,, to a,,, i.c. in the number of oxygen molecules produced per electron generated by PSII. The ratio ao,:@,, was highly variable at low light intensities (E < OSE,), and at higher light intensities (E > OSE,) @(,, : @,, showed a nonlinear decrease with increasing light intensity. In contrast, in P. globosa. a trend in a<,, : @,, could not be distinguished, and this species showed a decrease in @'o, : $ during the day, indicating a dependency of a(,, : a,, on light history. Additionally, considerable interspecific quantitative differences in a(,, : a,, were observed. Two possible interpretations to explain the variability in '.I+)! : a, are discussed. Assuming that 0, is a reliable measure of the quantum yield for charge separation at PSII, one interpretation is that net oxygen production is influenced by processes that consume oxygen or affect linear electron transport (e.g. cyclic electron transport around PSII, pseudocyclic electron transport in the Mehler reaction, Rubisco oxygenase activity, and lightdependent mitochondrial respiration). A second interpretation, however, suggests that at saturating light, changes in photosynthesis turnover time occur, such that $ does not predict the steady-state O2 yield.Quantum yields of phytoplankton photosynthesis are usually defined as the quantum yields for O? production (CacjL) or C fixation (@c,,,) (Kok 1948;Myers 1980;Babin et al. 1996). Because measurements of @oz and @'co, are laborious and time-consuming, much attention has been focused lately on the use of variable chlorophyll fluorescence as a tool to measure the quantum yield of charge separation in photosystem II (PSIl) reaction centers (Genty et al. 1989). This technique is rapid and noninvasive and may offer high temporal and spatial resolution when used in field measurements (Schreiber et al. 1986). Theoretically, the rate of noncyclic photosynthetic electron transport of a PSI1 (J) can be calculated from the quantum yield of PSI1 charge separation (a,,) according to J = @,J%s,,rwhere E is the incident light intensity and cr,,,, is the absorption cross section of PSII, which determines the fraction of the incident light intensity that is actually used by PSI1 (e.g. Kolber and Falkowski 1993; Kroon 1991; Hofstraat et al. 1994, Biehler andFock 1995). In order to use J as a measure for photosynthesis, the relationships between @,,, @coL, and @coz have to be well established. According to the stoichiometry of the Z-schem...

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Rates of respiration in the light measured in marine phytoplankton using an 18O isotope-labelling technique

Cited by 86 publications

References 50 publications

Diel cycles of planktonic respiration rates in briefly incubated water samples from a fertile earthen pond

Diel cycles of planktonic respiration rates in briefly incubated water samples from a fertile earthen pond

Response of coccolithophorid Emiliania huxleyi to elevated partial pressure of CO2 under nitrogen limitation

Light dependence of quantum yields for PSII charge separation and oxygen evolution in eucaryotic algae

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