Ratiometric Dual Signal-Enhancing-Based Electrochemical Biosensor for Ultrasensitive Kanamycin Detection

Abstract

Based on the signal amplification elements of planar VS2/AuNPs nanocomposites and CoFe2O4 nanozyme, we herein developed an electrochemical biosensor for sensitive kanamycin (Kana) quantification. A ratiometric sensing platform was presented by incorporating VS2/AuNPs nanocomposites as a support material with excellent conductivity and high specific surface area, as well as hairpin DNA (hDNA) with complementary hybridization of biotinylated Kana-aptamer. In addition, streptavidin-functionalized CoFe2O4 nanozyme with superior peroxidase-like catalytic activity were immobilized onto the aptasensor, hence the peroxidase-like catalytic reaction could yield amplified electrochemical signals. With the presence of Kana, the aptamer-biorecognition resulted in a quantitative decrease of nanozyme accumulation and an increase of methylene blue response. Under optimal conditions, the electrochemical signal ratio of the aptasensor revealed a linear relation along with the logarithmic concentration of Kana from 1 pM to 1 μM, with the limit of detection reaching to 0.5 pM. Moreover, this aptasensor exhibited excellent precision, as well as high repeatability, hence possessing potentials in real samples and for diverse targets detection by easy replacement of the matched aptamer.

Nanomaterials‐assisted metabolic analysis toward in vitro diagnostics

“…[52] The common strategy for incorporating nanozymes into electrochemical sensing systems is to develop nanomaterial hybrids with synergistic effect to enhance detection sensitivity. [53][54][55][56] For instance, the nafion/graphene oxide-gold nanoparticle (GO-AuNP) hybrid developed by Jin et al served as peroxidase mimics for building the electrode and detecting H 2 O 2 , which displayed an estimated LOD of 1.9 nmol within a linear detection range of 10-10 mmol. [53] In addition to monometallic nanozymes, many attempts were devoted to investigating the multi-metallic nanomaterials for electrochemical sensing.…”

Regulation Mechanism of ssDNA Aptamer in Nanozymes and Application of Nanozyme-Based Aptasensors in Food Safety

“…After magnetic separation, the supernatant containing numerous Pt nanozymes was used to catalyze the oxidation of TMB by H 2 O 2 . Moreover, Tian et al [ 108 ] reported a ratiometric dual signal-enhancing-based electrochemical aptasensor for ultrasensitive detection of kanamycin with a LOD of 0.5 pM ( Figure 4 F). The VS 2 /AuNPs nanocomposites were modified on the surface of a glassy carbon electrode (GCE), which remarkably accelerated the interface electron transfer.…”

“…( E ) A colorimetric bioassay for detecting kanamycin based on aptamer biorecognition-triggered hairpin switch and nicking-enzyme-assisted signal amplification [ 107 ]. ( F ) Ratiometric dual signal-enhancing-based electrochemical biosensor for ultrasensitive kanamycin detection [ 108 ]. Copyright 2020 American Chemical Society.…”

Nanozyme Applications: A Glimpse of Insight in Food Safety

“…The sensor can accurately and reproducibly detect tetracycline in drugs and milk in the range from 1 to 16 μM with a LOD of 46 nM. In addition, Tian et al established a ratio electrochemical biosensor for the quantitative detection of kanamycin based on signal amplification elements of planar VS 2 /AuNPs nanocomposites and CoFe 2 O 4 nanozyme ( Tian et al, 2020 ). The electrochemical aptasensor revealed a detection range from 1 pM to 1 μM with a LOD of 0.5 pM.…”