Ratiometric electrochemical aptasensor for ultrasensitive detection of Ochratoxin A based on a dual signal amplification strategy: Engineering the binding of methylene blue to DNA

Zhu, Chengxi; Liu, Dong; Li, Yuye; Shen, Xiuli; Ma, Shuai; Liu, Yang; You, Tianyan

doi:10.1016/j.bios.2019.111814

Cited by 82 publications

(25 citation statements)

References 32 publications

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“…The difference in intercalating properties of label molecules into single-stranded DNA compared to double-stranded DNA can be used to incorporate multiple label molecules into duplexes in a label amplification biosensor [76]. MB is more readily intercalated into double-stranded DNA than single-stranded DNA, and You et al used this property for the detection of Ochratoxin A (OTA) (Figure 23) [77]. A 3 -Fc-labelled DNA support probe, complementary to the aptamer for OTA, was immobilised onto a AuE.…”

Section: (F) Multiple Label Intercalationmentioning

confidence: 99%

Ratiometric Electrochemistry: Improving the Robustness, Reproducibility and Reliability of Biosensors

Spring

Goggins²,

Frost

2021

Molecules

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Electrochemical biosensors are an increasingly attractive option for the development of a novel analyte detection method, especially when integration within a point-of-use device is the overall objective. In this context, accuracy and sensitivity are not compromised when working with opaque samples as the electrical readout signal can be directly read by a device without the need for any signal transduction. However, electrochemical detection can be susceptible to substantial signal drift and increased signal error. This is most apparent when analysing complex mixtures and when using small, single-use, screen-printed electrodes. Over recent years, analytical scientists have taken inspiration from self-referencing ratiometric fluorescence methods to counteract these problems and have begun to develop ratiometric electrochemical protocols to improve sensor accuracy and reliability. This review will provide coverage of key developments in ratiometric electrochemical (bio)sensors, highlighting innovative assay design, and the experiments performed that challenge assay robustness and reliability.

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Section: (F) Multiple Label Intercalationmentioning

confidence: 99%

Ratiometric Electrochemistry: Improving the Robustness, Reproducibility and Reliability of Biosensors

Spring

Goggins²,

Frost

2021

Molecules

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“…The developed ratiometric aptasensor displayed a low LOD of 0.016 ng/ml and a dynamic range of 0.05-20 ng/ml for AFB 1 . By employing methylene blue and Fc as the redox probes, Zhu, Liu, et al (2020) developed another ratiometric EC aptasensor for OTA based on the dual-signal amplification strategy. Fc-labeled cDNA (Fc-cDNA), OTA aptamer, and complementary helper DNA (hDNA) were assembled stepwise on the gold electrode surface to form dsDNA, on which large amounts of methylene blue were attached, consequently producing a low oxidation current of Fc (I Fc ) and a high oxidation current of methylene blue (I methylene blue ).…”

Section: Competitive Mode Assaymentioning

confidence: 99%

Aptasensors for mycotoxins in foods: Recent advances and future trends

Hou

Jia

Sheng

et al. 2021

Comp Rev Food Sci Food Safe

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Mycotoxin contamination in foods has posed serious threat to public health and raised worldwide concern. The development of simple, rapid, facile, and cost‐effective methods for mycotoxin detection is of urgent need. Aptamer‐based sensors, abbreviated as aptasensors, with excellent recognition capacity to a wide variety of mycotoxins have attracted ever‐increasing interest of researchers because of their simple fabrication, rapid response, high sensitivity, low cost, and easy adaptability for in situ measurement. The past few decades have witnessed the rapid advances of aptasensors for mycotoxin detection in foods. Therefore, this review first summarizes the reported aptamer sequences specific for mycotoxins. Then, the recent 5‐year advancements in various newly developed aptasensors, which, according to the signal output mode, are divided into electrochemical, optical and photoelectrochemical categories, for mycotoxin detection are comprehensively discussed. A special attention is taken on their strengths and limitations in real‐world application. Finally, the current challenges and future perspectives for developing novel highly reliable aptasensors for mycotoxin detection are highlighted, which is expected to provide powerful references for their thorough research and extended applications. Owing to their unique advantages, aptasensors display a fascinating prospect in food field for safety inspection and risk assessment.

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Section: Strategies In Electrochemical Aptasensor Design: Aptamer Immobilization and Electrochemical Signal Generationmentioning

confidence: 99%

“…These electrochemical sensors are based on the ratiometric approach, a widely used detection mode in fluorescence analysis [ 119 ], providing superior reliability, accuracy, and reproducibility [ 120 , 121 ]. Ratiometric electrochemical sensors display two response signals, and the analyte is quantified by the ratio of these two signals [ 121 ]. As emphasized in recent reviews, the difference between a dual-signal electrochemical sensor and a ratiometric sensor is that the dual-signal sensor’s output is in fact the sum of the two electrochemical signals and not their ratio [ 120 , 122 ].…”

Section: Strategies In Electrochemical Aptasensor Design: Aptamer Immobilization and Electrochemical Signal Generationmentioning

confidence: 99%

“…However, according to the latest reports, increasing the number of redox-active labels, as in the case of a ratiometric procedure, leads only to a minimal boost in sensitivity [ 120 , 121 ]. A significant increase in sensitivity can be achieved through amplification strategies, which are compatible with ratiometric electrochemical detection methods [ 120 ].…”

Section: Strategies In Electrochemical Aptasensor Design: Aptamer Immobilization and Electrochemical Signal Generationmentioning

confidence: 99%

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The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

Raicopol

Pilan

2021

Materials

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Food safety monitoring assays based on synthetic recognition structures such as aptamers are receiving considerable attention due to their remarkable advantages in terms of their ability to bind to a wide range of target analytes, strong binding affinity, facile manufacturing, and cost-effectiveness. Although aptasensors for food monitoring are still in the development stage, the use of an electrochemical detection route, combined with the wide range of materials available as transducers and the proper immobilization strategy of the aptamer at the transducer surface, can lead to powerful analytical tools. In such a context, employing aryldiazonium salts for the surface derivatization of transducer electrodes serves as a simple, versatile and robust strategy to fine-tune the interface properties and to facilitate the convenient anchoring and stability of the aptamer. By summarizing the most important results disclosed in the last years, this article provides a comprehensive review that emphasizes the contribution of aryldiazonium chemistry in developing electrochemical aptasensors for food safety monitoring.

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Ratiometric electrochemical aptasensor for ultrasensitive detection of Ochratoxin A based on a dual signal amplification strategy: Engineering the binding of methylene blue to DNA

Cited by 82 publications

References 32 publications

Ratiometric Electrochemistry: Improving the Robustness, Reproducibility and Reliability of Biosensors

Ratiometric Electrochemistry: Improving the Robustness, Reproducibility and Reliability of Biosensors

Aptasensors for mycotoxins in foods: Recent advances and future trends

The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

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