Ratiometric fluorescent detection of exosomal piRNA-823 based on Au NCs/UiO-66-NH2 and target-triggered rolling circle amplification

Tong, Yao; Guan, Bingxin; Sun, Zhiwei; Dong, Xiangjun; Chen, Yuqing; Li, Yanru; Jiang, Yanyan; Li, Juan

doi:10.1016/j.talanta.2023.124307

Cited by 12 publications

(5 citation statements)

References 47 publications

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“…The induction of the overexpression of piR-823 has not yet been studied. It has recently been shown that piR-823 can be secreted by cancer cells via exosomes, and it was found in biopsies from patients with colorectal cancer [102]. Extrapolating from these data, piR-823 secreted by CSC during tumor development may be involved in the autocrine and paracrine maintenance of the stem phenotype in the cancer population.…”

Section: Role Of Ev Pirnas In Transformation Of Non-cscs Into Cscsmentioning

confidence: 89%

Tumor Microenvironment Modulation by Cancer-Derived Extracellular Vesicles

Ten,

Kumeiko,

Farniev

et al. 2024

Cells

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The tumor microenvironment (TME) plays an important role in the process of tumorigenesis, regulating the growth, metabolism, proliferation, and invasion of cancer cells, as well as contributing to tumor resistance to the conventional chemoradiotherapies. Several types of cells with relatively stable phenotypes have been identified within the TME, including cancer-associated fibroblasts (CAFs), tumor-associated macrophages (TAMs), neutrophils, and natural killer (NK) cells, which have been shown to modulate cancer cell proliferation, metastasis, and interaction with the immune system, thus promoting tumor heterogeneity. Growing evidence suggests that tumor-cell-derived extracellular vesicles (EVs), via the transfer of various molecules (e.g., RNA, proteins, peptides, and lipids), play a pivotal role in the transformation of normal cells in the TME into their tumor-associated protumorigenic counterparts. This review article focuses on the functions of EVs in the modulation of the TME with a view to how exosomes contribute to the transformation of normal cells, as well as their importance for cancer diagnosis and therapy.

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Section: Role Of Ev Pirnas In Transformation Of Non-cscs Into Cscsmentioning

confidence: 89%

Tumor Microenvironment Modulation by Cancer-Derived Extracellular Vesicles

Ten,

Kumeiko,

Farniev

et al. 2024

Cells

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“…They found significantly higher serum levels of piRNA-823 in cancer patients, compared to controls, with a significant correlation with tumor stage, tumor differentiation, and lymph node metastasis. Others have followed up on Sabbah's work, most recently an article which developed a nanotechnology-based detection platform for measuring piRNA-823 in clinical samples [16].…”

Section: Small Non-coding Rnasmentioning

confidence: 99%

Non-Coding RNAs in Human Health and Diseases

Good

2023

Genes

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Non-coding RNAs (ncRNAs) are, arguably, the enigma of the RNA transcriptome. Even though there are more annotated ncRNAs (25,967) compared to mRNAs (19,827), we know far less about each of the genes that produce ncRNA, especially in terms of their regulation, molecular functions, and interactions. Further, we are only beginning to understand the role of differential regulation or function of ncRNAs caused by genetic and epigenetic perturbations, such as single nucleotide variants (SNV), deletions, insertions, and histone/DNA modifications. The 22 papers in this Special Issue describe the emerging roles of ncRNAs in neurological, cardiovascular, immune, and hepatic systems, to name a few, as well as in diseases such as cancer, Prader–Willi Syndrome, cardiac arrhythmias, and diabetes. As we begin to understand the function and regulation of this class of RNAs, strategies targeting ncRNAs could lead to improved therapeutic interventions for some conditions.

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“…7 Moreover, a single-base difference can be well discriminated due to the high fidelity of the ligation reaction. The proposed nanosensor exhibits a low detection limit of 0.104 fM, superior to the reported piRNA assays, 8 a ,13 and it enables sensitive quantification of piRNA in different cell lines and even clinical tissue samples, providing a new platform for piRNA-related fundamental research and clinical diagnostics.…”

mentioning

confidence: 99%

“…The sensitivity of the proposed nanosensor has improved by 6 orders of magnitude compared with that of the DNA tetrahedron probe-based fluorescent method (0.5 nM), 8 a and 2 orders of magnitude compared with that of the rolling circle amplification-mediated fluorescent assay (10.2 fM). 13 The improvement of sensitivity can be attributed to: (1) efficient exponential signal amplification achieved by ligation chain reaction, (2) improved FRET efficiency caused by the assembly of multiple Cy5-labeled probes onto a single 605QD, and (3) highly sensitive single-molecule detection.…”

mentioning

confidence: 99%