Ratiometric imaging of butyrylcholinesterase activity in mice with nonalcoholic fatty liver using an AIE-based fluorescent probe

Xiang, Chunbai; Xiang, Jingjing; Xing, Yang; Li, Chunbin; Jiang, Daoyong; Peng, Yonglin; Xu, Zhen; Deng, Guanjun; Zhu, Baode; Zhang, Pengfei; Cai, Lintao; Gong, Ping

doi:10.1039/d2tb00422d

Cited by 37 publications

(13 citation statements)

References 56 publications

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“…Effective monitoring of the physiological progression of ALI is crucial for early theranostics to reduce its high mortality rate. Recent evidence suggests that the upstream–downstream linkages of esterase could be a critical factor for physiology studies of various diseases in vivo. − A few studies have been reported and validated for evaluating the dynamic changes of esterase in the lungs. , Given the above, the physiological progression of ALI can be further determined by detecting the dynamic changes of esterase. Accordingly, the novel probe FJH-C was rationally designed and synthesized by introducing the response group (cyclopropanecarbonyl chloride) at the phenolic hydroxyl position of FJH (Figure a) .…”

Section: Results and Discussionmentioning

confidence: 99%

De Novo Design of Activatable Photoacoustic/Fluorescent Probes for Imaging Acute Lung Injury In Vivo

et al. 2022

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Effective monitoring of the physiological progression of acute lung injury (ALI) in real time is crucial for early theranostics to reduce its high mortality. In particular, activatable fluorescence and photoacoustic molecule probes have attracted attention to assess ALI by detecting related indicators. However, the existing fluorophores often encounter issues of low retention in the lungs and slow clearance from the body, which compromise the probe’s actual capability for in situ imaging by intravenous injection in vivo. Herein, a novel near-infrared hemicyanines fluorophore (FJH) bearing a quaternary ammonium group was first developed by combining with the rational design and screening strategy. The properties of good hydrophilicity and blood circulation effectively enable FJH accumulation for lung imaging. Inspired by the high retention efficiency, the probe FJH-C that turns on fluorescence and photoacoustic signals in response to the ALI indicator (esterase) was subsequently synthesized. Notably, the probe FJH-C successfully achieved the selectivity and sensitivity toward esterase in vitro and in living cells. More importantly, FJH-C can be further used to assess lipopolysaccharides and silica-induced ALI through the desired fluo-photoacoustic signal. Therefore, this study not only shows the first activatable probe for real-time imaging of lung function but also highlights the fluorophore structure with high lung retention. It is believed that FJH and FJH-C can serve as an efficient platform to reveal the pathological progression of other lung diseases for early diagnosis and medical intervention.

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Section: Results and Discussionmentioning

confidence: 99%

De Novo Design of Activatable Photoacoustic/Fluorescent Probes for Imaging Acute Lung Injury In Vivo

et al. 2022

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“…Compared with conventional fluorescent probes, AIEgens show great advantages in the imaging of metabolic diseases. 91,171,172 To overcome the strong hydrophobicity that limits the efficiency of lipid-specific fluorophores for in vivo labeling in metabolic diseases, Liu et al synthesized two AIEgens (TPACN and TPAPhCN) with a typical D-A structure. Triphenylamine (TPA) served as the electron donor, while the dicyanovinyl group served as the electron acceptor.…”

Section: Metabolic Disease Bioimagingmentioning

confidence: 99%

Noncancerous disease-targeting AIEgens

et al. 2023

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“…It is associated with various diseases, such as liver disease, diabetes and Alzheimer’s disease [ 94 ]. Recently, Xiang et al designed a ratiometric fluorescent probe (TB-BChE) for imaging of BChE based on AIE mechanisms [ 95 ]. The TB-BChE probe showed a low intramolecular charge transfer effect because of the poor electron-donating ability of the ester group.…”

Section: Cholinesterasesmentioning

confidence: 99%

Biosensors for the Detection of Enzymes Based on Aggregation-Induced Emission

et al. 2022

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Enzymes play a critical role in most complex biochemical processes. Some of them can be regarded as biomarkers for disease diagnosis. Taking advantage of aggregation-induced emission (AIE)-based biosensors, a series of fluorogens with AIE characteristics (AIEgens) have been designed and synthesized for the detection and imaging of enzymes. In this work, we summarized the advances in AIEgens-based probes and sensing platforms for the fluorescent detection of enzymes, including proteases, phosphatases, glycosidases, cholinesterases, telomerase and others. The AIEgens involve organic dyes and metal nanoclusters. This work provides valuable references for the design of novel AIE-based sensing platforms.

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Ratiometric imaging of butyrylcholinesterase activity in mice with nonalcoholic fatty liver using an AIE-based fluorescent probe

Abstract: Butyrylcholinesterase (BChE) is an essential human biomarker which related to liver and neurodegenerative diseases, it is of great significance to develop a fluorescent probe that can image BChE in vitro...

Cited by 37 publications

References 56 publications

De Novo Design of Activatable Photoacoustic/Fluorescent Probes for Imaging Acute Lung Injury In Vivo

De Novo Design of Activatable Photoacoustic/Fluorescent Probes for Imaging Acute Lung Injury In Vivo

Noncancerous disease-targeting AIEgens

Biosensors for the Detection of Enzymes Based on Aggregation-Induced Emission

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