Rational design of gelatin/nanohydroxyapatite cryogel scaffolds for bone regeneration by introducing chemical and physical cues to enhance osteogenesis of bone marrow mesenchymal stem cells

Shalumon, K.T.; Liao, Han-Tsung; Kuo, Chang-Yi; Wong, Chak-Bor; Li, Chien-Ju; Mini, P A; Chen, Jyh‐Ping

doi:10.1016/j.msec.2019.109855

Cited by 43 publications

(32 citation statements)

References 63 publications

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“…Various implant materials for bone regeneration have been continually developed and designed to improve interaction with the patient's tissue at the cellular level through physical and chemical modification of the implant surface [36,37]. In particular, the PCL material approached in this study is a biodegradable and biocompatible polymer that is easy to fabricate in the form of a membrane and is suitable for studying the interaction between a cell and a substrate surface.…”

Section: Discussionmentioning

confidence: 99%

Lithographically-Fabricated HA-Incorporated PCL Nanopatterned Patch for Tissue Engineering

et al. 2020

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Inspired by the aligned extracellular matrix and bioceramics in human bone tissue, we investigated the relative contributions of nanotopography and equine bone powders (EBPs) with human dental pulp stem cells (DPSCs) to the osteogenesis. Both nanotopography and EBPs independently promoted the osteogenesis of DPSCs, osteogenesis was further promoted by the two factors in combination, indicating the importance of synergistic design factor of guided bone regeneration (GBR) membrane. The osteogenesis of DPSCs was affected by the polycaprolactone-based nanotopography of parallel nanogrooves as well as EBPs coating. Interestingly, both nanopattern and EBPs affected the DPSCs morphologies; nanopattern led to cell elongation and EBPs led to cell spreading and clustering. Analysis of the DPSCs-substrate interaction, DPSCs-EBPs interaction suggests that the combined environment of both factors play a crucial role in mediating osteogenic phenotype. This simple method to achieve a suitable environment for osteogenesis via nanotopography and EBPs coating modulation may be regarded as a promising technique for GBR/GTR membranes, which widely used dental and maxillofacial surgery applications.

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Section: Discussionmentioning

confidence: 99%

Lithographically-Fabricated HA-Incorporated PCL Nanopatterned Patch for Tissue Engineering

et al. 2020

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“…HyA is the major component of bone mineral matrix and is osteoinductive and osteoconductive, being able to induce stem cell differentiation into osteoblast-like cells [ 140 ]. The incorporation in nano-HyA into gelatin cryogels increased the ultimate stress under compression from 98 to 524 kPa [ 141 ]. In addition, the nano-HyA particles enhanced the expression of osteogenic markers in MSCs in vitro [ 141 , 142 ].…”

Section: Engineering Advanced Hydrogels For Tissue Engineering Appmentioning

confidence: 99%

“…The incorporation in nano-HyA into gelatin cryogels increased the ultimate stress under compression from 98 to 524 kPa [ 141 ]. In addition, the nano-HyA particles enhanced the expression of osteogenic markers in MSCs in vitro [ 141 , 142 ]. Likewise, the incorporation of nano-HyA into chitosan hydrogels was shown to enhance the in vitro mineral matrix deposition and expression of alkaline phosphatase in MSCs, and promoted the healing of critical-sized defects in rat tibia [ 143 ].…”

Section: Engineering Advanced Hydrogels For Tissue Engineering Appmentioning

confidence: 99%

Natural-Based Hydrogels for Tissue Engineering Applications

et al. 2020

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In the field of tissue engineering and regenerative medicine, hydrogels are used as biomaterials to support cell attachment and promote tissue regeneration due to their unique biomimetic characteristics. The use of natural-origin materials significantly influenced the origin and progress of the field due to their ability to mimic the native tissues’ extracellular matrix and biocompatibility. However, the majority of these natural materials failed to provide satisfactory cues to guide cell differentiation toward the formation of new tissues. In addition, the integration of technological advances, such as 3D printing, microfluidics and nanotechnology, in tissue engineering has obsoleted the first generation of natural-origin hydrogels. During the last decade, a new generation of hydrogels has emerged to meet the specific tissue necessities, to be used with state-of-the-art techniques and to capitalize the intrinsic characteristics of natural-based materials. In this review, we briefly examine important hydrogel crosslinking mechanisms. Then, the latest developments in engineering natural-based hydrogels are investigated and major applications in the field of tissue engineering and regenerative medicine are highlighted. Finally, the current limitations, future challenges and opportunities in this field are discussed to encourage realistic developments for the clinical translation of tissue engineering strategies.

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“…Specifically, tissue engineering involves the design and synthesis of three-dimensional (3D) matrices from biomaterials to provide a structural framework and to facilitate the attachment and migration of host cells, inducing a successful in vitro and in vivo regeneration of tissues [ 2 , 3 , 4 ]. Biomimetic 3D scaffolds may allow the control and application of a multi-stimulus to cells, including mechanical, electrical, and biochemical stimulations, in order to trigger specific responses, such as cell differentiation and tissue repair [ 5 , 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…Porosity also regulates the vascularization by angiogenesis and cell attachment [ 30 , 36 ]. Mechanical properties of biomaterials, such as stiffness, structure, and topography, are also considered during ECM synthesis, mainly because they can alter the local tissue microenvironments through intracellular and intercellular signaling [ 7 , 9 , 37 ]. Besides, one of the most relevant applications of polysaccharide-based aerogels is the capability of releasing drugs as controlled delivery systems.…”

Section: Introductionmentioning

confidence: 99%

Smart Porous Multi-Stimulus Polysaccharide-Based Biomaterials for Tissue Engineering

2020

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Recently, tissue engineering and regenerative medicine studies have evaluated smart biomaterials as implantable scaffolds and their interaction with cells for biomedical applications. Porous materials have been used in tissue engineering as synthetic extracellular matrices, promoting the attachment and migration of host cells to induce the in vitro regeneration of different tissues. Biomimetic 3D scaffold systems allow control over biophysical and biochemical cues, modulating the extracellular environment through mechanical, electrical, and biochemical stimulation of cells, driving their molecular reprogramming. In this review, first we outline the main advantages of using polysaccharides as raw materials for porous scaffolds, as well as the most common processing pathways to obtain the adequate textural properties, allowing the integration and attachment of cells. The second approach focuses on the tunable characteristics of the synthetic matrix, emphasizing the effect of their mechanical properties and the modification with conducting polymers in the cell response. The use and influence of polysaccharide-based porous materials as drug delivery systems for biochemical stimulation of cells is also described. Overall, engineered biomaterials are proposed as an effective strategy to improve in vitro tissue regeneration and future research directions of modified polysaccharide-based materials in the biomedical field are suggested.

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Rational design of gelatin/nanohydroxyapatite cryogel scaffolds for bone regeneration by introducing chemical and physical cues to enhance osteogenesis of bone marrow mesenchymal stem cells

Cited by 43 publications

References 63 publications

Lithographically-Fabricated HA-Incorporated PCL Nanopatterned Patch for Tissue Engineering

Lithographically-Fabricated HA-Incorporated PCL Nanopatterned Patch for Tissue Engineering

Natural-Based Hydrogels for Tissue Engineering Applications

Smart Porous Multi-Stimulus Polysaccharide-Based Biomaterials for Tissue Engineering

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