Rational design of Salmonella-based vaccination strategies

Spreng, Simone; Dietrich, Guido; Weidinger, Gerald

doi:10.1016/j.ymeth.2005.09.012

Cited by 53 publications

(43 citation statements)

References 85 publications

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“…Although routes of delivery of antigens expressed in recombinant Salmonella strains have been extensively explored in mice (32,33), there were few previous studies demonstrating that s.c. vaccination produced effective immune responses and protection based on this principle. In this study, the results from immunization experiments demonstrated that s.c., but not oral, vaccination with this strain provided complete protection against i.n.…”

Section: Discussionmentioning

confidence: 99%

“…The evaluation of different routes of immunization is an important way to modulate immune responses according to clinical requirements. The oral route of antigen delivery is the most common and most frequently explored among the mucosal immunization routes and stimulates both systemic and mucosal immune responses (32,33). In addition, other immunization routes have been extensively explored in mice, including nasal, rectal, vaginal, and intraperitoneal administration (for examples, see references 7 and 14).…”

mentioning

confidence: 99%

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Subcutaneous Vaccination with Attenuated Salmonella enterica Serovar Choleraesuis C500 Expressing Recombinant Filamentous Hemagglutinin and Pertactin Antigens Protects Mice against Fatal Infections with both S. enterica Serovar Choleraesuis and Bordetella bronchiseptica

Zhao

Xue

et al. 2008

Infect Immun

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Subcutaneous Vaccination with Attenuated Salmonella enterica Serovar Choleraesuis C500 Expressing Recombinant Filamentous Hemagglutinin and Pertactin Antigens Protects Mice against Fatal Infections with both S. enterica Serovar Choleraesuis and Bordetella bronchiseptica

Zhao

Xue

et al. 2008

Infect Immun

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“…In addition, the evaluation of different routes of immunization is an important way to modulate immune responses according to clinical requirements. The oral route of antigen delivery is the most common and frequent way of inducing effective immune responses (Sirard et al, 1999;Spreng et al, 2006), whereas there have been few studies concerning systemic immunity following intranasal vaccination based on this principle.…”

Section: Introductionmentioning

confidence: 99%

Oral and intranasal administration of somatostatin DNA vaccine mediated by attenuated Salmonella Enterica Serovar Typhimurium to promote growth of piglets

Liu

Liang

Feng

et al. 2011

Animal

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An attenuated strain of Salmonella typhimurium has been used as a carrier for oral and intranasal genetic immunization. Here, we evaluate the efficacy of a vaccine strain of S. typhimurium. CSO22 (pGM-CSF/SS, plasmid granulocyte-macrophage colonystimulating factor/somatostatin) expressing two copies of SS genes. A total of 115 piglets, aged 2 months old, were either orally or intranasally immunized against the vaccine strain CSO22 (pGM-CSF/SS) with three dosages (5 3 10 10 colony forming units (CFU), 5 3 10 9 CFU and 5 3 10 8 CFU). For oral immunization, the specific anti-SS antibodies were detected in the immunized piglets. The levels of SS antibodies in the high-dose immunized group (5 3 10 10 CFU) were significantly higher than that in the phosphate buffered saline immunized group (P , 0.01) and 40% of animals were positive in SS antibodies in the high-dose immunized group. Moreover, the weight gain of the high-dose group was increased by 20.86%, 10.26% and 15.30% during 4, 8 and 12 weeks, respectively, after immunization in comparison to the control. For intranasal immunization, the growth of the lowdose group was increased by 10.23% in the whole test period (12 weeks). In conclusion, our results suggest that the recombinant strain could elicit anti-SS antibodies and improve the growth performance of immunized piglets, and that the oral immunization program is better than the intranasal program.

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“…S. Typhimurium and S. Enteritidis are the most well-known examples and are able to cause different disease outcomes in various host species (Barrow, 2007, Boyle, et al, 2007, Lax, et al, 1995, Spreng, et al, 2006, Zhang & Mosser, 2008. S. Typhimurium and S. Enteritidis are able to induce a systemic infection in mice, young calves, chicks, and piglets.…”

mentioning

confidence: 99%

“…S. Typhimurium and S. Enteritidis are able to induce a systemic infection in mice, young calves, chicks, and piglets. However, they are also able to colonize poultry and adult cattle without symptoms (Barrow, 2007, Boyle, et al, 2007, Lax, et al, 1995, Spreng, et al, 2006, Zhang & Mosser, 2008. In humans, infection with either of these serovars results in a self-limiting gastroenteritis (salmonellosis) involving fever, diarrhea, and abdominal pain.…”

mentioning

confidence: 99%