Rational Development of Hypervalent Glycan Shield‐Binding Nanoparticles with Broad‐Spectrum Inhibition against Fatal Viruses Including SARS‐CoV‐2 Variants

Abstract

Infectious virus diseases, particularly coronavirus disease 2019, have posed a severe threat to public health, whereas the developed therapeutic and prophylactic strategies are seriously challenged by viral evolution and mutation. Therefore, broad‐spectrum inhibitors of viruses are highly demanded. Herein, an unprecedented antiviral strategy is reported, targeting the viral glycan shields with hypervalent mannose‐binding nanoparticles. The nanoparticles exhibit a unique double‐punch mechanism, being capable of not only blocking the virus–receptor interaction but also inducing viral aggregation, thereby allowing for inhibiting the virus entry and facilitating the phagocytosis of viruses. The nanoparticles exhibit potent and broad‐spectrum antiviral efficacy to multiple pseudoviruses, including severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) and its major variants (D614G, N501Y, N439K, Δ69‐70, Delta, and Omicron; lentiviruses expressing only the spike proteins), as well as other vital viruses (human immunodeficiency virus 1 and Lassa virus), with apparent EC50 values around the 10−9 m level. Significantly, the broad‐spectrum inhibition of authentic viruses of both wild‐type SARS‐CoV‐2 and Delta variants is confirmed. Therefore, this hypervalent glycan‐shield targeting strategy opens new access to broad‐spectrum viral inhibition.

ZIF‐67‐Derived Antivirus Cobalt Hydroxide LDH Nanosheets Produced through High‐Concentration Cobalt Ion‐Assisted Hydration

“…In comparison to other anti-SARS-CoV-2 nanomaterials, such as MUS CD (EC 50 : 34.7 μg ml −1 ), Graphene Oxide (EC 50 : >60 μg ml −1 ), NanoMIP (EC 50 : 37.54 μg ml −1 ), the CHN developed in this study demonstrates great advantages in terms of its antiviral efficacy. [53][54][55] To exclude the anti-viral effects that were wrongly associated with cytotoxicity, we also assessed the cell viability of CHN toward the host cells. As shown in Figure 9F,G, the cell viability of both cells remained largely unchanged up to 40 μg mL −1 , indicating good biocompatibility of CHN with these host cells.…”

Rational Supramolecular Strategy via Halogen Bonding for Effective Halogen Recognition in Molecular Imprinting

“…Molecular imprinting is a powerful technique to generate customized affinity pockets in a highly cross-linked polymer matrix for targeted molecules. In contrast to natural receptors, such as proteins and enzymes, artificial receptors exhibit many key advantages, such as low cost, robustness concerning physical stability and chemical inertness, and simplicity of preparation. − Therefore, molecular imprinting has gained popularity in many fields, including molecular sensing, − affinity chromatography, − pseudo-immunoassays, − precise disease diagnosis, smart/targeted drug delivery, − catalysis, − cancer immunotherapy, , and viral inhibition. , Generally, the first step of molecular imprinting is complex formation via selective chemical bonding, such as boronate affinity binding − or molecular self-assembly between templates and functional monomers. The complex is subsequently fixed by using an appropriate polymerization method in the presence of a cross-linking agent.…”

Branched polyethylenimine-assisted boronic acid functionalized magnetic nanoparticles for highly efficient capture of lincomycin and clindamycin

“…So far, some very important papers related to fluoro-phenylboronic acids have been reported. 28–34 However, there are no papers related to FFPBA yet. Furthermore, as compared with the low binding capacity of single boronic acid ligands, dendrimeric boronic acids can provide higher binding capacity toward cis -diols because dendrimeric boronic acids would amplify the number of boronic acid sites, which provides highly synergistic multiple binding.…”