Rational strategy for studying microbiome of the ocular surface of people using hard contact lenses by method of 16S rRNA gene metabarcoding

Аветисов, С Э; Abramova, N. D.; Gogoleva, Natalia; Gusev, Oleg; Mitichkina, T S; Ia, Novikov; Subbot, A.M.; Shagimardanova, Elena

doi:10.17116/oftalma20201360313

Cited by 8 publications

(2 citation statements)

References 20 publications

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“…Culture-independent approaches, such as sequencing of bacterial 16S ribosomal ribonucleic acid (rRNA), have recently been developed for the assessment of ocular surface microbiota. This is particularly useful because some microbial species require extremely specific growing conditions [9]. When compared to more conventional approaches to bacterial identification, 16S rRNA gene sequencing has proven to be a speedy and accurate way for diagnosing a wide range of eye illnesses caused by bacteria [10].…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic tree analysis based on the 16S sequence alignment for Klebsiella pneumonia and Enterobacter aerogenes Isolated from patients with Ocular eye infection in Wasit province, Iraq

Mukal Assaad Hussein,

Rana H Raheema,

Hassan Khalil Melek

2023

World J. Adv. Res. Rev.

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In Iraq and other developing countries, ocular infections are a significant public health concern, particularly gram-negative bacterial infections, which are common. The emergence of multidrug resistance is a critical concern because it can lead to treatment failure when bacterial strains become resistant to antimicrobial drugs. This study aimed to investigate, Phylogenetic tree analysis of Klebsiella pneumonia and Enterobacter aerogenes isolated from patients with Ocular eye infection. The tree provided evidence of a unique species that was represented by a single nucleic acid sequence. This species encompassed both Klebsiella pneumoniae and Enterobacter aerogenes. Through phylogenetic tree analysis, it was determined that the 16SrRNA gene exhibited effective discriminatory power in identifying these bacterial strains. Based on the sequencing data, it can be inferred that there is a significant sequence similarity between the Klebsiella pneumoniae samples and strains from Bangladesh. Similarly, Enterobacter aerogenes showed high sequence similarity with strains from India and Iraq, indicating a shared species affiliation.

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Section: Introductionmentioning

confidence: 99%

Phylogenetic tree analysis based on the 16S sequence alignment for Klebsiella pneumonia and Enterobacter aerogenes Isolated from patients with Ocular eye infection in Wasit province, Iraq

Mukal Assaad Hussein,

Rana H Raheema,

Hassan Khalil Melek

2023

World J. Adv. Res. Rev.

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“…Due to the demanding growth conditions of some microbial species, new technologies for the OSM evaluation included culture-independent methods, via sequencing of bacterial 16S ribosomal ribonucleic acid (rRNA). The latter allows the description of the entire microbial communities with their relative abundances [ 9 ]. Through sequencing data, it emerged that Staphylococcus spp.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Comparison of Ocular Surface Microbiome in Newborns

Petrillo

Marrapodi

et al. 2022

Microorganisms

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The ocular microbiome is of fundamental importance for immune eye homeostasis, and its alteration would lead to an impairment of ocular functionality. Little evidence is reported on the composition of the ocular microbiota of term infants and on the impact of antibiotic prophylaxis. Methods: A total of 20 conjunctival swabs were collected from newborns at birth and after antibiotic treatment. Samples were subjected to 16S rRNA sequencing via system MiSeq Illumina. The data were processed with the MicrobAT software and statistical analysis were performed using two-way ANOVA. Results: Antibiotic prophylaxis with gentamicin altered the composition of the microbiota. In detail, a 1.5- and 2.01-fold reduction was recorded for Cutibacterium acnes (C. acnes) and Massilia timonae (M. timonae), respectively, whereas an increase in Staphylococcus spp. of 6.5 times occurred after antibiotic exposure. Conclusions: Antibiotic prophylaxis altered the ocular microbiota whose understanding could avoid adverse effects on eye health.

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