Rational use of stacking principles for signal enhancement in capillary electrophoretic separations of poliovirus samples

Oita, Iuliana; Halewyck, Hadewych; Pieters, Sigrid; Dejaegher, Bieke; Thys, Bert; Rombaut, Bartholomeus; Heyden, Yvan Vander

doi:10.1016/j.jpba.2010.12.032

Cited by 10 publications

(15 citation statements)

References 42 publications

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“…Previous experience has demonstrated that an increase in poliovirus concentration produces a linear increase in poliovirus area, regardless of the sample plug length used for separation (Oita et al, 2009(Oita et al, , 2011. This was also observed when the cell extracts were spiked with a viral suspension purified by sucrose gradient (Fig.…”

Section: Estimation Of Poliovirus Concentration In Purified Cell Extrmentioning

confidence: 54%

“…Capillary electrophoresis was used to quantitate the poliovirus particles (Oita et al, 2011), and to distinguish the D-antigen from the heat denaturated poliovirus, non-exposing the D-antigen (Halewyck et al, 2010). Additionally, Okun et al (2000) demonstrated that another picornavirus, the human rhinovirus, was still viable after a similar capillary electrophoresis procedure.…”

Section: Discussionmentioning

confidence: 99%

“…The concentration of poliovirus in the cell extracts was expected to be much lower compared to the samples already analysed using capillary electrophoresis. Therefore, a signal enhancing approach (Oita et al, 2011) was used during the initial injection of the cell extracts. Since the electrophoretic behaviour of the cell extracts in our separation conditions was completely unknown, we chose to apply the signal enhancing approach in a conservative manner, i.e.…”

Section: Direct Injection Of the Cell Extractsmentioning

confidence: 99%

“…43 nL). Although this approach does not provide the highest signal enhancement, we preferred it as starting point in the cell extracts exploration because it has only a minimal effect on separation (Oita et al, 2011).…”

Section: Direct Injection Of the Cell Extractsmentioning

confidence: 99%

“…However, when the same separation conditions were used for samples with lower viral purity, and also during the study of the less abundant subviral particles, poliovirus was not detected anymore. An intra-capillary signal enhancement approach was proposed for such samples (Oita et al, 2011). The current research presents an approach for successful detection of poliovirus in infected cell extracts, downstream of the purification process, using a label free, capillary electrophoretic method with large sample plug injections.…”

Section: Introductionmentioning

confidence: 99%

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Poliovirus separation from cell extracts using capillary electrophoresis: Potential use in vaccine production and control?

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Section: Estimation Of Poliovirus Concentration In Purified Cell Extrmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Direct Injection Of the Cell Extractsmentioning

confidence: 99%

Section: Direct Injection Of the Cell Extractsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Poliovirus separation from cell extracts using capillary electrophoresis: Potential use in vaccine production and control?

Oita

Halewyck

Pieters

et al. 2012

Journal of Virological Methods

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Contemporary sample stacking in analytical electrophoresis

et al. 2012

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Sample stacking is a term denoting a multifarious class of methods and their names that are used daily in CE for online concentration of diluted samples to enhance separation efficiency and sensitivity of analyses. The essence of these methods is that analytes present at low concentrations in a large injected sample zone are concentrated into a short and sharp zone (stack) in the separation capillary. Then the stacked analytes are separated and detected. Regardless of the diversity of the stacking electromigration methods, one can distinguish four main principles that form the bases of nearly all of them: (i) Kohlrausch adjustment of concentrations, (ii) pH step, (iii) micellar methods, and (iv) transient ITP. This contribution is a continuation of our previous reviews on the topic and brings an overview of papers published during 2010-2012 and relevant to the mentioned principles (except the last one which is covered by another review in this issue).

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Recent advances in capillary electrophoretic migration techniques for pharmaceutical analysis

et al. 2014

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Since the introduction about 30 years ago, CE techniques have gained a significant impact in pharmaceutical analysis. The present review covers recent advances and applications of CE for the analysis of pharmaceuticals. Both small molecules and biomolecules such as proteins are considered. The applications range from the determination of drug-related substances to the analysis of counterions and the determination of physicochemical parameters. Furthermore, general considerations of CE methods in pharmaceutical analysis are described.

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Rational use of stacking principles for signal enhancement in capillary electrophoretic separations of poliovirus samples

Cited by 10 publications

References 42 publications

Poliovirus separation from cell extracts using capillary electrophoresis: Potential use in vaccine production and control?

Poliovirus separation from cell extracts using capillary electrophoresis: Potential use in vaccine production and control?

Contemporary sample stacking in analytical electrophoresis

Recent advances in capillary electrophoretic migration techniques for pharmaceutical analysis

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