Rationale behind the optimum efficiency of columns packed with new 1.9μm fully porous particles of narrow particle size distribution

Catani, Martina; Ismail, Omar H.; Cavazzini, Alberto; Ciogli, Alessia; Villani, Claudio; Pasti, Luisa; Bergantin, Caterina; Cabooter, Deirdre; Desmet, Gert; Gasparrini, Francesco; Bell, David S.

doi:10.1016/j.chroma.2016.05.037

Cited by 53 publications

(56 citation statements)

References 45 publications

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“…Overall,

c_{s}

terms are remarkably small for all the three columns, indicating that mass transfer kinetics from the stationary to the mobile phase is fast. They are even slightly larger on the SPP‐2.0 column, most likely due to its smaller pores with respect to those of FPP columns [35, 63].…”

Section: Resultsmentioning

confidence: 99%

“…In this work, three UHPLC C 18 columns with the same dimensions (100 × 3 mm, L × id) but packed with particles of different geometry were used. Titan C 18 column (FPP‐1.9) packed with monodisperse 1.9 μm FPPs, characterized by a narrow particle size distribution with an RSD of about 6% [35] (120 Å pore size, 300 m 2 /g specific surface area), was generously donated by Supelco Analytical (Merck, USA). Acquity BEH C18 (FPP‐1.7) packed with 1.7 μm FPPs (130 Å pore size, 185 m 2 /g specific surface area) was from Waters (Milford, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…(), describes the band broadening related to the irregularities of the stream path in the packed bed. It includes trans‐channel eddy dispersion, short‐range interchannel eddy dispersion, and trans‐column eddy dispersion [35]. In achiral systems,

a (ν)

can be determined by subtracting the values of b and

c_{s}

(estimated by Eqs.…”

Section: Theorymentioning

confidence: 99%

“…Some of the author of this work recently explored the kinetic performance of two columns packed with sub‐2 μm nPSD C18 FPPs with different pore size. Results of that investigation revealed an extremely low eddy dispersion in both cases [35, 36].…”

Section: Introductionmentioning

confidence: 99%

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Investigation of mass transfer properties and kinetic performance of high‐efficiency columns packed with C₁₈ sub‐2 μm fully and superficially porous particles

Felletti

Luca

Lievore

et al. 2020

J of Separation Science

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Three columns packed with 2.0 μm superficially porous particles, 1.7 μm fully porous particles, and monodisperse 1.9 μm fully porous particles with narrow particle size distribution have been deeply characterized from a kinetic point of view. The 1.9 μm column showed excellent kinetic performance, comparable to that of the superficially porous one. These two columns also exhibit flatter c-branches of the van Deemter curve compared to the 1.7 μm fully porous particles column, resulting in smaller loss of efficiency when they are operated at higher flow rates than the optimal ones. The independent evaluation of each contribution to band broadening has revealed that the difference in kinetic performance comes from the very small eddy dispersion contribution on the 1.9 μm column, surprisingly even lower than that of the superficially porous one. This finding suggests a very good packing of the monodisperse 1.9 μm column. On the other hand, the potential of 1.7 μm fully porous particles is completely broken down by the strong frictional heating effect already arising at relatively low flow rates. K E Y W O R D Sfully porous particles, kinetic performance, octadecyl stationary phase, superficially porous particles, ultrahigh-performance liquid chromatography

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“…Overall,

c_{s}

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

a (ν)

can be determined by subtracting the values of b and

c_{s}

(estimated by Eqs.…”

Section: Theorymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Investigation of mass transfer properties and kinetic performance of high‐efficiency columns packed with C₁₈ sub‐2 μm fully and superficially porous particles

Felletti

Luca

Lievore

et al. 2020

J of Separation Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, at the moment, the model is not implemented in any dedicated standalone software, and because of the quite complex mathematics involved, it is unlikely that such an approach may routinely be used in DC experiments. Nevertheless, the MSM model could attract specific interest on the basis of its ability to correlate macroscopic classical chromatographic parameters with the behavior properties of individual molecules (Pasti et al, 2005(Pasti et al, , 2016Felinger et al, 2005).…”

Section: Chapter 3 Chiral Separationsmentioning

confidence: 99%

Theory and Practice of UHPLC and UHPLC–MS

Guillarme

Veuthey

2017

Handbook of Advanced Chromatography /Mass Spectrometry Techniques

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Microfluidic Precision Manufacture of High Performance Liquid Chromatographic Microspheres

Sun,

Zeng,

Liu

et al. 2024

Angewandte Chemie

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A key bottleneck in developing chromatographic material is the chemically entangled control of morphology, pore structure, and material chemistry, which holds back precision material manufacture in order to pursue advanced separation performance. In this work, a precision manufacture strategy based on droplet microfluidics was developed, for production of highly efficient chromatographic microspheres with independent control over particle morphology, pore structure and material chemistry. The droplet‐synthesized microspheres display extremely narrow particle size distribution (CV<3%), enabling a 100% production yield due to complete elimination of sieving steps. More importantly, the size of the droplet‐synthesized microspheres is freely adjustable without the need for re‐optimizing chemical recipes or reaction conditions. The resulting materials exhibit excellent separation efficiencies, achieving a reduced plate height of hmin=1.67. This precision manufacture strategy also allows for flexible pore design and continuous pore size adjustment across three orders of magnitudes, providing a novel vehicle for resolution fine‐tuning targeting protein separation. Besides traditional silica, organic‐inorganic hybrid silica, zirconia, and titania microspheres can also be precisely synthesized on the same platform, supporting various separation applications and operating conditions. Powered by precision manufacture, super‐throughput production, and versatile chemistry, the high‐performance droplet‐synthesized separation material will pave the way towards green and precision chromatographic industry.

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Rationale behind the optimum efficiency of columns packed with new 1.9μm fully porous particles of narrow particle size distribution

Cited by 53 publications

References 45 publications

Investigation of mass transfer properties and kinetic performance of high‐efficiency columns packed with C₁₈ sub‐2 μm fully and superficially porous particles

Investigation of mass transfer properties and kinetic performance of high‐efficiency columns packed with C₁₈ sub‐2 μm fully and superficially porous particles

Theory and Practice of UHPLC and UHPLC–MS

Microfluidic Precision Manufacture of High Performance Liquid Chromatographic Microspheres

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Rationale behind the optimum efficiency of columns packed with new 1.9μm fully porous particles of narrow particle size distribution

Cited by 53 publications

References 45 publications

Investigation of mass transfer properties and kinetic performance of high‐efficiency columns packed with C18 sub‐2 μm fully and superficially porous particles

Investigation of mass transfer properties and kinetic performance of high‐efficiency columns packed with C18 sub‐2 μm fully and superficially porous particles

Theory and Practice of UHPLC and UHPLC–MS

Microfluidic Precision Manufacture of High Performance Liquid Chromatographic Microspheres

Contact Info

Product

Resources

About

Investigation of mass transfer properties and kinetic performance of high‐efficiency columns packed with C₁₈ sub‐2 μm fully and superficially porous particles

Investigation of mass transfer properties and kinetic performance of high‐efficiency columns packed with C₁₈ sub‐2 μm fully and superficially porous particles