Rationalization of specific structure formation in electrospinning process: Study on nano‐fibrousPCL‐ andPLGA‐based scaffolds

Abstract

Formation of specific structures on poly-ɛ-caprolactone (PCL) and poly lactide-co-glycolide (PLGA) based electrospun mats is rationalized and the effect of interactive parameters; high voltage and flow rate on unique surface topography is evaluated. By increasing the collecting time in electrospinning process and enhancing fiber to fiber repulsion, surface characteristics of mats changes from nano- to micro-topography. In this study surface topography of the fabricated mats based on PCL and PLGA were assessed using AFM and SEM techniques to display the distinct phenomenon occurs on collected random fibers. In this research the rationale behind the formation of bump and flower like structures on fibrous mats was discussed. Because of great potential application of the fabricated substrates in the fields of medical purposes, cell-matrix interaction was evaluated and in vitro biological test with human dermal fibroblast and mouse L929 fibroblast cells was performed to study the cell responses to different roughness of nano-fibers collected at different time intervals. Our results show that after 7 days, cell proliferation is improved on PCL collected at 40 min in the case of human fibroblast cells and on PCL collected in 70 min in the case of L929 mouse fibroblast cells.

Effect of electrospinning parameters on morphological properties of PVDF nanofibrous scaffolds

“…Among these techniques, electrospinning of the polymers into fibrous meshes with combination of nanoscale and microscale fibers has exhibited close physical characteristics of the extracellular matrix of cells. Electrospinning has been widely studied in neural tissue engineering due to its flexible setup which can be modulated depending on the polymer’s and scaffold’s features (Greiner and Wendorff 2007 ; Lee and Arinzeh 2011 ; Pham et al 2006 ; Saeed et al 2015 ).…”

Comparative of fibroblast and osteoblast cells adhesion on surface modified nanofibrous substrates based on polycaprolactone

“…This result demonstrated the improvement of the cells’ behavior on the substrate roughness compared to the substrate without roughness (Farooque et al 2012). In this study, electrospun PCL-based nano-fibers which have been produced by various time intervals of spinning provide different roughness (Mirzadeh et al 2015). As it can be seen in Fig.…”

“…Although bio-substrate provides desired microenvironments for cell seeding, but hydrophobic nature of PCL gives rise to low cell loading in the initiatory step of the cell culture leading to less cellular adhesion, migration, and proliferation (Besunder et al 2010; Lee et al 2008). Plasma treatment results in physical and chemical changes by introducing new chemical functional groups, improvement in hydrophilicity and surface permeability (Besunder et al 2010; Lee et al 2008; Mirzadeh et al 2015). To improve the biocompatibility, PCL substrate with different roughness was selected (Chan et al 2008) and treated with O 2 plasma technique.…”

Piezoelectric electrospun nanocomposite comprising Au NPs/PVDF for nerve tissue engineering

“…Electrospinning is a versatile and cost‐effective technique for the production of multi‐functional nanofibers from various polymers, polymer blends, composites, sol‐gels, ceramics, and so forth with diameters ranging from several micrometers down to a few nanometers …”

“…Electrospinning is a versatile and cost-effective technique for the production of multi-functional nanofibers from various polymers, polymer blends, composites, sol-gels, ceramics, and so forth with diameters ranging from several micrometers down to a few nanometers. 26,27 These nanofibers have nanoscale diameters, high surface-to-volume ratio, porosity and unique physicochemical properties for different and wide range of applications such as tissue engineering scaffolds and drug delivery systems. 11,[28][29][30][31] The basic principle of this technique is based on generating the direct movement of charged molecules by applying high voltage to supply the ejection of a liquid jet through spinneret from the polymer solution droplet.…”