Rationally Designed Synthetic Vectors for Gene Delivery

Rao, Gururaj; Yadava, Preeti; Hughes, Jeffrey A.

doi:10.2174/187412660701017066

Cited by 4 publications

(6 citation statements)

References 57 publications

(60 reference statements)

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“…Thus, present research is aimed at designing gene delivery agents that are able to deliver DNA into the cells with minimal toxicity [28]. Gelatins showed low cytotoxicity towards HeLa cells, which displayed viability in the range 77% to 100% and 84 and 100%, as determined by the MTT and NRU assays, respectively.…”

Section: Cytotoxicity In Vitromentioning

confidence: 99%

Gelatin-based nanoparticles as DNA delivery systems: Synthesis, physicochemical and biocompatible characterization

Morán

Rosell

Ruano

et al. 2015

Colloids and Surfaces B: Biointerfaces

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The rapidly rising demand for therapeutic grade DNA molecules requires associated improvements in encapsulation and delivery technologies. One of the challenges for the efficient intracellular delivery of therapeutic biomolecules after their cell internalization by endocytosis is to manipulate the non-productive trafficking from endosomes to lysosomes, where degradation may occur. The combination of the endosomal acidity with the endosomolytic capability of the nanocarrier can increase the intracellular delivery of many drugs, genes and proteins, which, therefore, might enhance their therapeutic efficacy. Among the suitable compounds, the gelification properties of gelatin as well as the strong dependence of gelatin ionization with pH makes this compound an interesting candidate to be used to the effective intracellular delivery of active biomacromolecules. In the present work, gelatin (either high or low gel strength) and protamine sulfate has been selected to form particles by interaction of oppositely charged compounds. Particles in the absence of DNA (binary system) and in the presence of DNA (ternary system) have been prepared. The physicochemical characterization (particle size, polydispersity index and degree of DNA entrapment) have been evaluated. Cytotoxicity experiments have shown that the isolated systems and the resulting gelatin-based nanoparticles are essentially non-toxic. The pH-dependent hemolysis assay and the response of the nanoparticles co-incubated in buffers at defined pHs that mimic extracellular, early endosomal and late endo-lysosomal environments demonstrated that the nanoparticles tend to destabilize and DNA can be successfully released. It was found that, in addition to the imposed compositions, the gel strength of gelatin is a controlling parameter of the final properties of these nanoparticles. The results indicate that these gelatin-based nanoparticles have excellent properties as highly potent and non-toxic intracellular delivery systems, rendering them promising DNA vehicles to be used as non-viral gene delivery systems.

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Section: Cytotoxicity In Vitromentioning

confidence: 99%

Gelatin-based nanoparticles as DNA delivery systems: Synthesis, physicochemical and biocompatible characterization

Morán

Rosell

Ruano

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…Thus, present research is aimed at designing gene delivery agents that are able to deliver DNA 412 into the cells with minimal toxicity (Rao et al, 2007). 413…”

Section: Cytotoxic Assessments 406mentioning

confidence: 99%

Mixed protein–DNA gel particles for DNA delivery: Role of protein composition and preparation method on biocompatibility

Morán

Nogueira

Vinardell

et al. 2013

International Journal of Pharmaceutics

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“…Unfortunately, while high concentrations of the delivery agents imply an increased chance of the DNA penetrating the cell nucleus, they can also interfere with physiological processes within the cell, inducing cell death. Thus, present research is aimed at designing gene delivery agents that are able to deliver DNA into the cells with minimal toxicity [101].…”

Section: Cytotoxicitymentioning

confidence: 99%

DNA gel particles: An overview

Morán

Vinardell

Infante

et al. 2014

Advances in Colloid and Interface Science

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A general understanding of interactions between DNA and oppositely charged compounds forms the basis for developing novel DNA-based materials, including gel particles. The association strength, which is altered by varying the chemical structure of the cationic cosolute, determines the spatial homogeneity of the gelation process, creating DNA reservoir devices and DNA matrix devices that can be designed to release either single-(ssDNA) or double-stranded (dsDNA). This review covers recent developments on the topic of DNA gel particles formed in water-water emulsion-type interfaces. The degree of DNA entrapment, particle morphology, swelling/dissolution behaviour and DNA release responses are discussed as a function of the nature of the cationic agent used. On the basis of designing DNA gel particles for therapeutic purposes, recent studies on the determination of the surface hydrophobicity, the haemolytic and the cytotoxic assessments of the obtained DNA gel particles have been also reported.

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Rationally Designed Synthetic Vectors for Gene Delivery

Cited by 4 publications

References 57 publications

Gelatin-based nanoparticles as DNA delivery systems: Synthesis, physicochemical and biocompatible characterization

Gelatin-based nanoparticles as DNA delivery systems: Synthesis, physicochemical and biocompatible characterization

Mixed protein–DNA gel particles for DNA delivery: Role of protein composition and preparation method on biocompatibility

DNA gel particles: An overview

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