2007
DOI: 10.1128/mcb.02187-06
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Rct1, a Nuclear RNA Recognition Motif-Containing Cyclophilin, Regulates Phosphorylation of the RNA Polymerase II C-Terminal Domain

Abstract: Phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (RNAP II) is a dynamic process that regulates transcription and coordinates it with pre-mRNA processing. We show here that Rct1, a nuclear multidomain cyclophilin from Schizosaccharomyces pombe, is encoded by an essential gene that interacts with the CTD and regulates its phosphorylation in vivo. Downregulation of Rct1 levels results in increased phosphorylation of the CTD at both Ser2 and Ser5 and in a commensurate decrease in RNAP II transcr… Show more

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Cited by 14 publications
(30 citation statements)
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“…Since phosphorylation of selected nucleo-cytosolic RRM proteins has been shown to be biologically important in metazoans (e.g. Huang and Steitz, 2005) and plants (Ali and Reddy, 2006;Gullerova et al, 2007), it seems likely that the modified cpRNPs could also play regulatory roles.…”
Section: Protein Modifications Of Cprnpsmentioning
confidence: 99%
“…Since phosphorylation of selected nucleo-cytosolic RRM proteins has been shown to be biologically important in metazoans (e.g. Huang and Steitz, 2005) and plants (Ali and Reddy, 2006;Gullerova et al, 2007), it seems likely that the modified cpRNPs could also play regulatory roles.…”
Section: Protein Modifications Of Cprnpsmentioning
confidence: 99%
“…In this experiment, an Rct1 knock out strain of S. pombe was used which is complemented by an HA-tagged Rct1 gene [7] and a CLIP experiment performed …”
Section: Example Experimentsmentioning
confidence: 99%
“…The protein used for these investigations was spRct1; this has been described as RNA Recognition Motif Containing Cyclophilin Regulating Transcription, as it is suggested to possess possible functions in activities connecting transcription and premRNA processing [7]. spRct1 is a highly conserved multidomain protein which consists of a peptidyl-prolyl cis-trans isomerase (PPIase) domain, followed by an RNA recognition motif (RRM), and a C-terminal domain enriched in charged amino acids.…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…This protein was named Rct1 (RRM-containing cyclophilin regulating transcription) because of the conserved RNA recognition motif (RRM), the Prolyl-Peptidyl Isomerase (PPIase) domain, and its role in regulating Pol II (Gullerova et al, 2007). It was first isolated as an essential SR domain protein (AtCyp59) in Arabidopsis , where it interacts with both Pol II and with other SR proteins that mediate alternative splicing (Gullerova et al, 2006, Thomas et al, 2012), and binds most cellular mRNA (Bannikova et al, 2013).…”
Section: Introductionmentioning
confidence: 99%