Re-emergence of mayaro virus and coinfection with chikungunya during an outbreak in the state of Tocantins/Brazil

Marinho, Robson dos Santos Souza; Duro, Rodrigo Lopes Sanz; Caldeira, Débora Bellini; Galinskas, Juliana; Mota, Mânlio Tasso Oliveira; Hunter, James R.; Teles, Maria da Aparecida Rodrigues; Milagres, Flavio Augusto de Pádua; Diaz, Ricardo Sobhie; Kawakubo, Fernando Shinjí; Komninakis, Shirley Vasconcelos

doi:10.1186/s13104-022-06153-6

Cited by 13 publications

(7 citation statements)

References 34 publications

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“…MAYV is endemic to the Amazon region. An epidemiological study between 1955 and 2019 reported 1304 cases of MAYV infection in Brazil, with 1142 cases described only in the northern region [ 4 ], which includes the states of Pará, Amazonas [ 35 ], and Tocantins [ 36 ]. Ma.…”

Section: Discussionmentioning

confidence: 99%

Report of natural Mayaro virus infection in Mansonia humeralis (Dyar & Knab, Diptera: Culicidae)

et al. 2023

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Background Arboviruses are a group of viruses transmitted to vertebrate hosts by certain blood-feeding arthropods. Among urban vectors of arboviruses, mosquitoes of the genus Aedes are the most common. However, other mosquitoes may be susceptible to infection and involved in the transmission, such as Mansonia spp. Therefore, this study aimed to investigate whether Mansonia humeralis can be infected with the Mayaro virus (MAYV). Methods These insects were collected from 2018 to 2020 in chicken coops of rural communities in Jaci Paraná in Porto Velho, Rondônia, Brazil, while performing blood-feeding on roosters. The mosquitoes were randomly grouped in pools from which the head and thorax were macerated and checked for the presence of MAYV by quantitative reverse transcription polymerase chain reaction (RT-qPCR). The positive pools were used to infect the C6/36 cell line, and on different days post-infection, the supernatant of the infected cells was subjected to viral detection by RT-qPCR. Results A total of 183 pools of female mosquitoes were tested, of which 18% were positive for MAYV; some samples from insect pools inoculated into C6/36 cells showed in vitro multiplication capacity between 3 and 7 days post-infection. Conclusions This is the first report of Ma. humeralis mosquitoes that are naturally infected by MAYV, indicating that these vectors may be potential transmitting agents of this arbovirus. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Report of natural Mayaro virus infection in Mansonia humeralis (Dyar & Knab, Diptera: Culicidae)

et al. 2023

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“…With the spread of arboviruses [ 8 , 17 ] and the increase in human cases, there have been a growing number of patients presenting co-infection combinations of CHIKV, DENV and ZIKV [ 18 ] and recently reports of MAYV with DENV [ 19 ], CHIKV [ 20 , 21 ] and ZIKV [ 22 ]. While the observations of multiple infections in same patients indicate that Ae.…”

Section: Introductionmentioning

confidence: 99%

Temperature affects viral kinetics and vectorial capacity of Aedes aegypti mosquitoes co-infected with Mayaro and Dengue viruses

Terradas,

Manzano-Alvarez,

Vanalli

et al. 2024

Parasites Vectors

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Background Increasing global temperatures and unpredictable climatic extremes have contributed to the spread of vector-borne diseases. The mosquito Aedes aegypti is the main vector of multiple arboviruses that negatively impact human health, mostly in low socioeconomic areas of the world. Co-circulation and co-infection of these viruses in humans have been increasingly reported; however, how vectors contribute to this alarming trend remains unclear. Methods Here, we examine single and co-infection of Mayaro virus (D strain, Alphavirus) and dengue virus (serotype 2, Flavivirus) in Ae. aegypti adults and cell lines at two constant temperatures, moderate (27 °C) and hot (32 °C), to quantify vector competence and the effect of temperature on infection, dissemination and transmission, including on the degree of interaction between the two viruses. Results Both viruses were primarily affected by temperature but there was a partial interaction with co-infection. Dengue virus quickly replicates in adult mosquitoes with a tendency for higher titers in co-infected mosquitoes at both temperatures, and mosquito mortality was more severe at higher temperatures in all conditions. For dengue, and to a lesser extent Mayaro, vector competence and vectorial capacity were higher at hotter temperature in co- vs. single infections and was more evident at earlier time points (7 vs. 14 days post infection) for Mayaro. The temperature-dependent phenotype was confirmed in vitro by faster cellular infection and initial replication at higher temperatures for dengue but not for Mayaro virus. Conclusions Our study suggests that contrasting kinetics of the two viruses could be related to their intrinsic thermal requirements, where alphaviruses thrive better at lower temperatures compared to flaviviruses. However, more studies are necessary to clarify the role of co-infection at different temperature regimes, including under more natural temperature settings. Graphical Abstract

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“…Due to co-circulation with other arboviruses, clinical disease similarity, and alphavirus cross-reactivity, these infections can also be difficult to diagnose as differentiating diagnostics are limited. There are incidences of arboviral co-infections, including reports of MAYV and CHIKV co-infection [ 32 ] and ex vivo superinfection with MAYV and Zika (ZIKV) [ 17 ]. Co-infections with non-arboviruses like HIV have also been reported, but little research has been done to investigate the interplay of these co-infections or consequence of pre-existing immunodeficiency [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

Mayaro virus pathogenesis and immunity in rhesus macaques

Weber,

Labriola,

Kreklywich

et al. 2023

PLoS Negl Trop Dis

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Mayaro virus (MAYV) is a mosquito-transmitted alphavirus that causes debilitating and persistent arthritogenic disease. While MAYV was previously reported to infect non-human primates (NHP), characterization of MAYV pathogenesis is currently lacking. Therefore, in this study we characterized MAYV infection and immunity in rhesus macaques. To inform the selection of a viral strain for NHP experiments, we evaluated five MAYV strains in C57BL/6 mice and showed that MAYV strain BeAr505411 induced robust tissue dissemination and disease. Three male rhesus macaques were subcutaneously challenged with 105 plaque-forming units of this strain into the arms. Peak plasma viremia occurred at 2 days post-infection (dpi). NHPs were taken to necropsy at 10 dpi to assess viral dissemination, which included the muscles and joints, lymphoid tissues, major organs, male reproductive tissues, as well as peripheral and central nervous system tissues. Histological examination demonstrated that MAYV infection was associated with appendicular joint and muscle inflammation as well as presence of perivascular inflammation in a wide variety of tissues. One animal developed a maculopapular rash and two NHP had viral RNA detected in upper torso skin samples, which was associated with the presence of perivascular and perifollicular lymphocytic aggregation. Analysis of longitudinal peripheral blood samples indicated a robust innate and adaptive immune activation, including the presence of anti-MAYV neutralizing antibodies with activity against related Una virus and chikungunya virus. Inflammatory cytokines and monocyte activation also peaked coincident with viremia, which was well supported by our transcriptomic analysis highlighting enrichment of interferon signaling and other antiviral processes at 2 days post MAYV infection. The rhesus macaque model of MAYV infection recapitulates many of the aspects of human infection and is poised to facilitate the evaluation of novel therapies and vaccines targeting this re-emerging virus.

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Re-emergence of mayaro virus and coinfection with chikungunya during an outbreak in the state of Tocantins/Brazil

Cited by 13 publications

References 34 publications

Report of natural Mayaro virus infection in Mansonia humeralis (Dyar & Knab, Diptera: Culicidae)

Report of natural Mayaro virus infection in Mansonia humeralis (Dyar & Knab, Diptera: Culicidae)

Temperature affects viral kinetics and vectorial capacity of Aedes aegypti mosquitoes co-infected with Mayaro and Dengue viruses

Mayaro virus pathogenesis and immunity in rhesus macaques

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