Re-evaluation of in vitro activity of primycin against prevalent multiresistant bacteria

A crotonyl-CoA reductase-carboxylase independent pathway for assembly of unusual alkylmalonyl-CoA polyketide synthase extender units

“…These clusters also contain genes encoding homologues of SamR0482. One of these genes clusters has recently been reported to direct the biosynthesis of the primycin complex of antibiotics24, which has been used in the clinic as a topical antibiotic and has recently been shown to be effective against multi-drug resistant Gram-positive pathogens, including methicillin- and mupirocin-resistant Staphylococcus aureus 25. Butylmalonyl-CoA is incorporated into primycin A1 ( 41 ) (Fig.…”

Structural and functional comparison of Saccharomonospora azurea strains in terms of primycin producing ability

“…The overexpression of FA synthesis related genes in low-producer S. azurea DSM 44631 strain suggests that biosynthetic activity of FA and polyketide related machinery is not a clear sequential process, rather should be considered an overlapping even at least partially. Primycin, a non-polyene marginolactone antibiotic produced by filamentous bacteria S. azurea, possesses high antimicrobial activity against frequent G+ pathogens, including clinically prevalent multidrug-resistant strains (Feiszt et al 2014). To protect themselves against their own bioactive metabolites, self-resistance mechanism for antibiotic producers are crucial.…”

The genus Micromonospora as a model microorganism for bioactive natural product discovery

“…[31][32][33] Later in 1998, it was found that rustamicin (18) and its congeners mediate their fungicidal activity via inhibition of the fungal inositol phosphoceramide synthase resulting in interrupted sphingolipid biosynthesis. 34 In 1980, further eighteen compounds called mycinamicin I-XVIII (26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43) were added to the 16-membered macrolide antibiotics family from a soil derived-M. griseorubida strain. Interestingly, mycinamicins showed potent bacteriostatic activity against Gram-positive pathogenic bacteria (MIC 0.1-3.12 mg mL À1 ).…”

“…42 In 2014, this interesting antibiotic was re-evaluated against prevalent multi-resistant Gram-positive bacteria responsible for common skin infections. 43 Besides its promising antibiotic activities, perimycin (45) possesses signicant antifungal effects against a wide range of pathogenic fungi. 44 Its complex formation with the fungal cell membrane ergosterol is considered the primary mode of action responsible for its antifungal properties.…”