Re-examination of Chromosomes of Three Species of Rice Planthoppers(Homoptera : Delphacidae)

Noda, Hiroaki; Tatewaki, Reiko

doi:10.1303/aez.25.538

Cited by 23 publications

(22 citation statements)

References 6 publications

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“…Linkage groups 16 and 17 in the paper were merged to our linkage groups 9 and 15, respectively. Therefore, the total number of linkage groups in the new BPH genetic map was reduced to 15, the same number as the chromosomes of this species [33]. Because of the very low recombination frequency in the BF 1 population, the location of SSR and SNP markers in each chromosome could not be analysed.…”

Section: (F ) Bulk Segregant Analysis With Next-generation Sequencingmentioning

confidence: 99%

Genetic mapping of the rice resistance-breaking gene of the brown planthopper Nilaparvata lugens

et al. 2014

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Host plant resistance has been widely used for controlling the major rice pest brown planthopper (BPH, Nilaparvata lugens). However, adaptation of the wild BPH population to resistance limits the effective use of resistant rice varieties. Quantitative trait locus (QTL) analysis was conducted to identify resistance-breaking genes against the anti-feeding mechanism mediated by the rice resistance gene Bph1. QTL analysis in iso-female BPH lines with single-nucleotide polymorphism (SNP) markers detected a single region on the 10th linkage group responsible for the virulence. The QTL explained from 57 to 84% of the total phenotypic variation. Bulked segregant analysis with next-generation sequencing in F 2 progenies identified five SNPs genetically linked to the virulence. These analyses showed that virulence to Bph1 was controlled by a single recessive gene. In contrast to previous studies, the genefor-gene relationship between the major resistance gene Bph1 and virulence gene of BPH was confirmed. Identified markers are available for map-based cloning of the major gene controlling BPH virulence to rice resistance.

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Section: (F ) Bulk Segregant Analysis With Next-generation Sequencingmentioning

confidence: 99%

Genetic mapping of the rice resistance-breaking gene of the brown planthopper Nilaparvata lugens

et al. 2014

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“…The presence of a male-specific sequence supports the conclusion of the previous cytological reports that male brown planthopper N. lugens has an XY sex chromosome system. Noda and Tatewaki (1990) reported that two other rice planthopper species, the small brown planthopper Laodelphax striatellus and the whitebacked planthopper Sogatella furcifera, do not possess Y chromosomes, that is, the sex determination system of theses species is XX in the female and XO in the male. The reason for the presence of a Y chromosome and its function in the brown planthopper, N. lugens, remain unknown.…”

Section: Discussionmentioning

confidence: 99%

“…lugens has XX chromosomes in the female and XY in the male (Noda and Tatewaki, 1990). To date, however, Y-specific DNA sequences have not been identified.…”

Section: Introductionmentioning

confidence: 99%

Identification of Y chromosomal PCR marker and production of a selected strain for molecular sexing in the brown planthopper, Nilaparvata lugens

Kobayashi

Noda

2007

Arch Insect Biochem Physiol

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A laboratory colony was established in order to enable molecular sexing in premature stages in the brown planthopper, Nilaparvata lugens. We found four male-specific amplified fragment length polymorphisms (AFLPs) in the planthopper, and sequenced one of the AFLPs along with its 5' flanking region (1,423 bp in total). PCR primers were designed based on the nucleotide sequence information so that the PCR product was present in male planthoppers and absent in female planthoppers. However, we could not completely distinguish males from females, because the PCR amplification product was absent in some of the males screened. We, therefore, established a laboratory colony, in which all males carried this sequence. We can directly sex pre-adult stages in this colony using our PCR primers, making this strain of considerable value for studies that require sex separation in egg and nymphal stages.

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“…Serangga ini merupakan organisme diploid dengan jumlah kromosom 30 (28 autosom dengan 2 kromosom X pada betina, atau 1 kromosom X dan 1 kromosom Y pada jantan (Noda 1990) dan ukuran genomnya sebesar 1,2 Gbp (Xue et al 2014). Rekonstruksi filogenetik menggunakan 318 gen-gen ortholog dengan 15 serangga dari taksa berbeda menunjukkan bahwa WBC berada dalam satu gerombol dengan serangga penusuk pengisap Rhodnius prolixus yang termasuk ke dalam ordo Hemiptera sehingga WBC dikeluarkan dari ordo Homoptera dan dimasukkan ke dalam ordo Hemiptera (Xue et al 2014).…”

Section: Strategi Hidup Dan Makan Wbcunclassified

Virulensi Wereng Batang Cokelat (Nilaparvata lugens Stål) dan Strategi Pengelolaannya

Chaerani¹

2018

J. AgroBiogen

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Brown planthopper (BPH) emerged as serious pest of rice in Asia as a result of green revolution. BPH outbreak is supported by its monofagous feeding behavior on rice, high fecundity, and ability to long distance migration. BPH is labile in virulence and capable of rapid adaptation on new resistant varieties. The terminology 'biotype' to describe BPH virulence is still debatable because of individual virulence and genetic variation within a biotype. This paper reviews the biology, biochemistry, interaction with endosymbionts, and genetics aspects of BPH to reveal virulence basis of BPH for improvement of BPH management strategies. At the molecular level, differential expression profiles of genes related to digestion, saliva secretion, detoxification, metabolisms of lipid, carbohydrate, amino acid, and nitrogen; signaling pathways of defense, stress, and immunity responses were noted among BPH population with differential virulence. Research in genomics revealed complementary interaction between BPH and endosymbiotic microorganisms, but experiments using aposymbiotic BPH showed that endosymbiont densities do not always positively correlated with BPH virulence. In contrast to the previous finding, recent genetic mapping of BPH Avr gene using inbred populations revealed that BPH virulence is monogenically controlled. With this finding, a gene-for-gene relationship between BPH and its host can been established. Strategies for utilizing resistant varieties to anticipate virulence adaptation according to the dynamics of resistant rice varieties deployed in the field are discussed in this paper.Keywords: Brown planthopper, virulence, biotype, adaptation, endosymbiont. ABSTRAKWereng batang cokelat (WBC) timbul menjadi hama utama padi di Asia sebagai dampak revolusi hijau. Ledakan populasi WBC didukung oleh perilaku WBC yang monofagus pada padi, keperidian tinggi, dan kemampuan migrasi jarak jauh. WBC bersifat labil dalam virulensi dan mampu beradaptasi pada varietas tahan. Penggunaan istilah 'biotipe' untuk pengelompokan virulensi WBC masih diperdebatkan karena adanya variasi virulensi dan genetik antarindividu dalam satu biotipe. Makalah tinjauan ini membahas aspek strategi hidup dan makan, biokimia, interaksi dengan endosimbion, dan genetik WBC untuk mengetahui basis adaptasi virulensi WBC sehingga dapat digunakan dalam perbaikan strategi pengelolaan hama ini. Pada level molekuler, virulensi ditandai dengan peningkatan profil ekspresi gen-gen yang berkaitan dengan detoksifikasi senyawa alelokemik tanaman; metabolisme lipid, karbohidrat, asam amino, dan nitrogen; jalur lintas penyinalan respons pertahanan, respons terhadap cekaman, dan respons kekebalan. Penelitian genomik mengungkap interaksi komplementer antara WBC dan mikroorganisme endosimbion, tetapi percobaan menggunakan WBC aposimbiotik menunjukkan bahwa kepadatan endosimbion tidak selalu berkorelasi positif dengan virulensi WBC. Bertolak belakang dengan hasil-hasil penelitian sebelumnya, pemetaan genetik gen virulensi menggunakan tetua inbred menunjukk...

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Re-examination of Chromosomes of Three Species of Rice Planthoppers(Homoptera : Delphacidae)

Cited by 23 publications

References 6 publications

Genetic mapping of the rice resistance-breaking gene of the brown planthopper Nilaparvata lugens

Genetic mapping of the rice resistance-breaking gene of the brown planthopper Nilaparvata lugens

Identification of Y chromosomal PCR marker and production of a selected strain for molecular sexing in the brown planthopper, Nilaparvata lugens

Virulensi Wereng Batang Cokelat (Nilaparvata lugens Stål) dan Strategi Pengelolaannya

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