Reaction mechanism of melatonin oxidation by reactive oxygen species in vitro

Bonnefont‐Rousselot, Dominique; Collin, Fabrice; Jore, D.; Gardès-Albert, Monique

doi:10.1111/j.1600-079x.2010.00847.x

Cited by 144 publications

(127 citation statements)

References 43 publications

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“…Investigations of the chemical properties of melatonin, both in vivo and in vitro, indicate that melatonin and its metabolites are potent antioxidants that directly scavenge both reactive oxygen species (ROS) and reactive nitrogen species (RNS) in the cells. 4,6,16 Besides, melatonin stimulates many antioxidant enzymes. The antioxidant properties of melatonin are connected with its neuroprotective activity in several degenerative disorders.…”

Section: Discussionmentioning

confidence: 99%

The effect of melatonin on spinal cord after ischemia in rats

et al. 2015

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Study design: Experimental animal model to assess ischemic spinal cord injury (SCI) following occlusion of the thoracoabdominal aorta. Objectives: In the present study, we aimed to investigate the role of melatonin on SCI induced by ischemia and following reperfusion. Setting: Animal Research Laboratory, Inonu University, Malatya, Turkey. Methods: We evaluated oxidative damage and caspase-3 activity. In total, 32 adult Wistar albino rats were divided into four groups: Group 1, control (n = 8); Group 2 (n = 8), those subjected to ischemia/reperfusion (IR) by clamping the thoraco-abdominal aorta; Group 3 (n = 8), melatonin (50 mg kg −1 ) treated; and Group 4 (n = 8), melatonin (50 mg kg −1 ) followed by ischemia. All animals were kept alive for 48 h, and then spinal cord samples were removed. We assayed oxidative damage by measuring malondialdehyde (MDA), apoptosis by measuring activated caspase-3 (using immunoblots) and intrinsic antioxidative capacity by measuring reduced glutathione (GSH) levels in the spinal cord. Results: The results indicated a significant decrease in activity of caspase-3 in SCI animals after treatment with melatonin, as it significantly decreased the formation of MDA and decelerated the loss of GSH. Conclusion: This study suggested that melatonin could be an effective neuroprotective agent for treatment of SCI.

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Section: Discussionmentioning

confidence: 99%

The effect of melatonin on spinal cord after ischemia in rats

et al. 2015

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“…AFMK is a newly identified biomarker in the serum of HD patients. It has been reported to be a strong free radical scavenger, to exert antioxidant activity, and can eliminate reactive oxygen and nitrogen species [11,13]. The elevation of serum AFMK may indicate enhanced oxidative stress in HD patients.…”

Section: Serum Metabolic Profile Of Hd Patients Differs Significantlymentioning

confidence: 99%

“…3). AFMK produced by melatonin oxidation is formed actively in inflammation [11]. In turn, AMK is formed by the deformylation of AFMK [12].…”

Section: Fecal and Serum Metabolic Differences Between Hd Patients Anmentioning

confidence: 99%

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Metabolite Profiling of Feces and Serum in Hemodialysis Patients and the Effect of Medicinal Charcoal Tablets

Liu

Liang

Liu

et al. 2018

Kidney Blood Press Res

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Background/Aims: Recently, the colon has been recognized as an important source of various uremic toxins in patients with end stage renal disease. Medicinal charcoal tablets are an oral adsorbent that are widely used in patients with chronic kidney disease in China to remove creatinine and urea from the colon. A parallel fecal and serum metabolomics study was performed to determine comprehensive metabolic profiles of patients receiving hemodialysis (HD). The effects of medicinal charcoal tablets on the fecal and serum metabolomes of HD patients were also investigated. Methods: Ultra-performance liquid chromatography/mass spectrometry was used to investigate the fecal and serum metabolic profiles of 20 healthy controls and 31 HD patients before and after taking medicinal charcoal tablets for 3 months. Results: There were distinct metabolic variations between the HD patients and healthy controls both in the feces and serum according to multivariate data analysis. Metabolic disturbances of alanine, aspartate and glutamate metabolism, arginine and proline metabolism figured prominently in the serum. However, in the feces, alterations of tryptophan metabolism, lysine degradation and beta-alanine metabolism were pronounced, and the levels of several amino acids (leucine, phenylalanine, lysine, histidine, methionine, tyrosine, and tryptophan) were increased dramatically. Nineteen fecal metabolites and 21 serum metabolites were also identified as biomarkers that contributed to the metabolic differences. Additionally, medicinal charcoal treatment generally enabled the serum and fecal metabolomes of the HD patients to draw close to those of the control subjects, especially the serum metabolic profile. Conclusion: Parallel fecal and serum metabolomics uncovered the systematic metabolic variations of HD patients, especially disturbances in amino acid metabolism in the colon. Medicinal charcoal tablets had an impact on the serum and fecal metabolomes of HD patients, but their exact effects still need to be studied further.

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“…6,7 Vitamin D3 is taken in the diet or produced in the skin from 7-dehydrocholesterol in the skin by ultraviolet irradiation. 8 Vitamin D3 is transported in the circulation by the DBP (vitamin D binding protein).…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Melatonin and 1,25-Dihydroxyvitamin D3 on Renal Ischemia–Reperfusion Injury in Rats

et al. 2013

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Ischemia-reperfusion (I/R) injury induces the generation of reactive oxygen species (ROS) which affect many organs. This study was designed to investigate the roles of melatonin and 1,25-dihydroxyvitamin D 3 (VD3) on renal I/R injury. Thirty male Wistar albino rats were divided into five groups: group 1, control; group 2, right nephrectomy (RN) þ I/R in the contralateral kidney; group 3, melatonin þ RN þ I/R; group 4, VD 3 þ RN þ I/R; and group 5, melatonin þ VD 3 þ RN þ I/R. Melatonin (10 mg/kg), VD3 (0.5 μg/kg), and melatonin plus VD3 were injected intraperitoneally for 7 days before renal I/R. After 7 days, right nephrectomy was initially performed and left renal artery was clamped for 45 min. After 45-min reperfusion, the serum and kidney tissue samples were obtained for assays. Melatonin and VD3 had an ameliorative effect on biochemical parameters such as serum creatinine (SCr) and blood urea nitrogen (BUN). Renal tissue malondialdehyde (MDA), glutathione (GSH), nitric oxide (NO) levels, and superoxide dismutase (SOD) activity were determined. Renal I/R decreased the kidney tissue GSH levels and SOD activity and increased the NO levels as compared with control group. However, melatonin and VD3 and melatonin plus VD3 treatment significantly increased the tissue GSH levels and SOD activity and decreased the NO levels compared with those of I/R group. Meanwhile, MDA levels were not different between the control and I/R groups. But, MDA levels decreased in all treated groups compared to I/R and control groups. These data support that melatonin and VD3 have beneficial effects on renal injury.

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Reaction mechanism of melatonin oxidation by reactive oxygen species in vitro

Cited by 144 publications

References 43 publications

The effect of melatonin on spinal cord after ischemia in rats

The effect of melatonin on spinal cord after ischemia in rats

Metabolite Profiling of Feces and Serum in Hemodialysis Patients and the Effect of Medicinal Charcoal Tablets

Protective Effect of Melatonin and 1,25-Dihydroxyvitamin D3 on Renal Ischemia–Reperfusion Injury in Rats

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