Reaction of Cd7-metallothionein with cis-dichlorodiammine platinum (II).

Reaction of Zn7Metallothionein with cis- and trans-[Pt(N-donor)2Cl2] Anticancer Complexes: trans-PtII Complexes Retain Their N-Donor Ligands

“…Therefore, in parallel to the kinetics of formation of Cys−Pt II bonds, the kinetics of Zn II release was also examined. Pt II binding experiments were repeated in the presence of the Zn II -chelating dye 4-(2-pyridylazo)resorcinol (PAR) monitoring the absorption of Zn(PAR) 2 complex at 500 nm. , Prior to these experiments the absence of a complex between PAR and Pt II was established. The change in PAR absorption with time is summarized in Figure a and b.…”

Interactions of Antitumor Metallodrugs with Serum Proteins: Advances in Characterization Using Modern Analytical Methodology

“…Metallothionein. With the objective to elucidate the roles of cisplatin−protein interactions in the mechanism of action, toxicity, and resistance of the drug, the interactions of cisplatin and metallothionein, a small cysteine-rich metal binding protein with a diversity of physiological functions, were studied by several research groups. ,, In the reaction of cisplatin with Cd 7 −MT (MT = metallothionein) followed by 111 Cd NMR spectroscopy, platinum displaced cadmium in an approximately 1:1 fashion, producing Pt 7 −MT have investigated the binding of cisplatin to metallothionein under conditions mimicking passage of clinical drug concentrations through the cytosol.…”

“…25,26,108 In the reaction of cisplatin with Cd 7 -MT (MT ) metallothionein) followed by 111 Cd NMR spectroscopy, platinum displaced cadmium in an approximately 1:1 fashion, producing Pt 7 -MT. 108 Applying a combination of HPLC, AAS, and UV-vis techniques, Hagrman et al 25 have investigated the binding of cisplatin to metallothionein under conditions mimicking passage of clinical drug concentrations through the cytosol. In particular, HPLC eluates were found containing increasedwith-reaction-time amounts of undialyzable Pt corresponding to the Pt-metallothionein product.…”

Reactivity of an antimetastatic organometallic ruthenium compound with metallothionein-2: relevance to the mechanism of action

“…However, these metals can be displaced by other metal ions in vivo that have a higher affinity for thiolates such as Cd(II), Hg(II), and Pt(II), and the molecular mechanisms of the Pt(II) interactions with MT-1/-2 have been the subject of numerous studies. [33][34][35][36] As far as we are aware, studies on the interaction of MTs with ruthenium compounds are lacking, despite the growing interest in ruthenium anticancer drugs and their ability to function in platinum-resistant tumours. Herein, we describe the use of electrospray ionisation mass spectrometry (ESI MS) to probe the interactions of RAPTA-C with rabbit MT-2, using cisplatin as a reference compound.…”