Reaction of I-131 Trace Labeled Human Anti-Rh0(d) With Red Cells*†

RELATIONSHIP BETWEEN Rh0(D) GENOTYPE AND QUANTITY OF I131 ANTI-Rh0(D) BOUND TO RED CELLS*

“…Except for the procedures described below, the methods presented in a previous paper (7) were used for iodination,' determination of radioactivity, stroma preparation and reaction of the red cells with the P"' anti-Rho(D).…”

“…High titered anti-Rho(D) serum was obtained from a 30 year old white housewife who had been immunized to the Rh0(D) antigen by multiple pregnancies. The pertinent details of her history and immunization have been presented (7).…”

Relationship between Rh0(D) Zygosity and Red Cell Rh0(D) Antigen Content in Family Members*

“…The isotopic methods described previously (3,13) were used except for the procedures described below.…”

“…The heat eluate derived from preparation A-79 was obtained by sensitizing a pool of lyophilized D-positive red cell stromata with the 'I-labeled globulin fraction at 370 C for 90 minutes. The sensitized stromata were washed four times and eluted by using the techniques described previously (3,13). A total of 80 g of stromata derived from a pool consisting of 34 U of Rho(D)-positive red cells was used, and 0.31 mg globulin nitrogen per g of lyophilized stromata was used for sensitization.…”

“…The cell-bound nitrogen under the conditions of reaction used in this study (equilibrium value) was about 80% of the extrapolated value. The assumptions involved in converting radioactivity to nitrogen have been discussed previously (3,11,13).…”