Reaction of the purine and pyrimidine derivatives with the electrode mercury

Paleček, Emil; Jelen, František; Hung, Mac Anh; Lasovský, Jan

doi:10.1016/s0022-0728(81)80262-8

Cited by 10 publications

(8 citation statements)

References 20 publications

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“…CSV of nucleic acid bases is based on the formation of sparingly soluble compounds with the electrode mercury, and represents a very sensitive technique [3,7], which was earlier used for determination of nucleic acids bases [8 -10]. Glodowski et al [8] presented determination of traces of purine by CSV at the hanging copper amalgam drop electrode and showed that purine is accumulated on the electrode surface as a Cu(I) -purine complex.…”

Section: Introductionmentioning

confidence: 99%

Microanalysis of DNA by stripping transfer voltammetry

Jelen

Kouřilová

Pečínka

et al. 2004

Bioelectrochemistry

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Section: Introductionmentioning

confidence: 99%

Microanalysis of DNA by stripping transfer voltammetry

Jelen

Kouřilová

Pečínka

et al. 2004

Bioelectrochemistry

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“…It was observed that the presence of a small quantity of acridine orange in a diluted alkaline electrolyte caused a slightly negative shift in the stripping peak of cytosine compared to the results obtained by Palecek and coworkers (Palecek et al 1981). The peak potential for cytosine in the NaOH solution containing acridine orange (E p ¼ 20.1 V) appears to be better than the peak potential in the borate buffer medium (E p ¼ 0.0 V) as reported by Meyeralmes and Porschke (1993), once it is located farther away from the mercury wave.…”

Section: Discussionmentioning

confidence: 51%

“…Analytical determinations of purine and pyrimidine derivatives have been performed by using polarography methods, and these were described 44 years ago by Smith and Elving (1962). In the 80 s, Palecek and coworkers used a borate buffer (pH 10.1) to quantify very low levels of these base derivatives by cathodic stripping voltammetric procedures at stationary mercury electrodes (Palecek et al 1981). In recent years, the aforementioned researchers have exhaustively studied the electrochemical behavior of DNA, mostly by using adsorptive stripping square-wave voltammetry (Jelen et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Cathodic Stripping Voltammetric Behavior of Cytosine in Presence of Acridine Orange at the Static Mercury Drop Electrode

et al. 2006

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Cathodic adsorptive electrochemical behavior of cytosine in the presence of acridine orange (DNA-intercalating dye) at the static mercury drop electrode was investigated. A 0.001 M NaOH solution containing 2 ppm of acridine orange was found to be the optimal supporting electrolyte. Other optimum experimental conditions were found to be as follows: the initial potential at 0.00 V, linear n, and the potential scan rate at 50 mV s 21 . The analytical response was found to be linear in a range of cystosine concentrations from 0.30 ppm to 1.1 ppm. For a direct response, the detection limit was 1.2 ppb. The presence of ssDNA and the interference of some trace metals were also investigated.

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“…In this context, Palecek [1][2][3][4] investigated the interaction of various purine and pyrimidine derivatives with a charged mercury electrode surface. Procedures based on this type of interaction have been proposed for the anodic stripping analysis of pesudouracil.…”

mentioning

confidence: 99%

“…Procedures based on this type of interaction have been proposed for the anodic stripping analysis of pesudouracil. 4 The cathodic adsorptive stripping voltammetric technique has been applied for the determination of a wide variety of nucleic acid bases 3,5,6 and their derivatives. [7][8][9] However, the interaction of such interesting biological compounds with metal ions has indicated that the chemical bond with the mercury is not only the case for stripping analysis technique.…”

mentioning

confidence: 99%

Differential Pulse and Square-Wave Cathodic Stripping Voltammetry of Xanthine and Xanthosine at a Mercury Electrode

et al. 2003

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Adsorptive stripping voltammetry, based on adsorptive accumulation, is very sensitive technique for the determination of antibiotics, drugs and organic substances of biologically significance.In this context, Palecek 1-4 investigated the interaction of various purine and pyrimidine derivatives with a charged mercury electrode surface. Procedures based on this type of interaction have been proposed for the anodic stripping analysis of pesudouracil. 4 The cathodic adsorptive stripping voltammetric technique has been applied for the determination of a wide variety of nucleic acid bases 3,5,6 and their derivatives. [7][8][9] However, the interaction of such interesting biological compounds with metal ions has indicated that the chemical bond with the mercury is not only the case for stripping analysis technique.These compounds can be determined to ultra trace level as a complex form with voltammetrically active metal ions. [10][11][12] In a continuation of our previous studies 6-9,12 on the cathodic adsorptive stripping voltammetric determination of some nucleic acid components, it would be of interest to investigate the adsorption and preconcentration for ultratrace voltammertic measurements of Xan and Xano under the optimum conditions. The adsorption and preconcentration studies were followed by the application of DPCASV and SWCASV techniques for a trace determination of the investigated compounds. The step of determination is based on a strong interaction of the adsorbed Xan or Xano species with the mercury electrode surface forming the Hg(II)-xanthate compound. The adsorption and preconcentration of the Cu(II)-xanthate complex at the mercury electrode surface was also investigated. Experimental InstrumentationA Princeton Applied Research (PAR) Model 174A polarographic analyzer coupled with a PAR 174/50 ac polarographic interface and a PAR Model 510 (Lock-inamplifier) phase detector were used for ac voltammetric measurements. A PAR model 264 was used for cyclic and cathodic adsorptive stripping voltammertic measurements. PAR models 303 HMDE and an advanced X-Y recorder (model RE0151), equipped with the aforementioned instruments were used for all measurements. The voltammetric cell was maintained at 22 + 0.5˚C (5˚C for ac measurements). A saturated Ag/AgCl electrode and Pt wire electrode were used as reference and auxiliary electrodes. A stir-head bar and a PAR Model 305 stirrer were also used for the cyclic and stripping voltammetric techniques. Reagents and proceduresXan and Xano were obtained from Sigma and were used without further purification. Solutions containing different concentrations of the investigated compounds were prepared by dissolving a known amount of the chemically pure product in a specific volume of twice-distilled water. Britton-Robinson (B.R) buffer was brought to a constant ionic strength of 0.5 mol -1 by the addition of NaNO3, and adjusted to the desired pH. All of the chemicals were of reagent grade (Merck, Darmstadt). All metal salts were in the nitrate form. Twice-distilled deionized w...

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Reaction of the purine and pyrimidine derivatives with the electrode mercury

Cited by 10 publications

References 20 publications

Microanalysis of DNA by stripping transfer voltammetry

Microanalysis of DNA by stripping transfer voltammetry

Cathodic Stripping Voltammetric Behavior of Cytosine in Presence of Acridine Orange at the Static Mercury Drop Electrode

Differential Pulse and Square-Wave Cathodic Stripping Voltammetry of Xanthine and Xanthosine at a Mercury Electrode

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