Reactions of the carbon tetrachloride-related peroxy free radical (CCl3O2.) with amino acids : Pulse radiolysis evidence

Packer, John E.; Slater, T. F.; Willson, R. L.

doi:10.1016/0024-3205(78)90378-8

Cited by 203 publications

(71 citation statements)

References 4 publications

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“…Since the CCl302" species is very reactive (Packer et al, 1978(Packer et al, , 1980, then it probably would not be effectively trapped by MNP in the concentrations that can be used in these experiments. Moreover, the adduct formed from CC1302 and MNP would be expected to show a hyperfine splitting aN of approx.…”

Section: Resultsmentioning

confidence: 99%

“…Although radiation studies (Tomasi et al, 1980;Symons et al, 1982) have demonstrated a small but significant change in the PBN-CC13 adduct when 02 iS present (notably a decrease in the aN hyperfine splitting constants and lack of 13C hyperfine splitting), no definite evidence could be found for this adduct under biological conditions. The most likely candidate for this particular adduct is the PBN-CC13 adduct formed via an interaction of the highly electrophilic CC1302 species (Packer et al, 1978(Packer et al, , 1980 (Tomasi et al, 1980 The results shown in Table 1 indicate some areas of concern that should be carefully evaluated before interpretation ofthe results obtained with spin traps in biologically complex systems. The spin traps studied in the present work, MNP and PBN, both interact with cytochrome P-450 to give difference spectra, and both have inhibitory effects on the mixedfunction oxidation of aminopyrene.…”

Section: Resultsmentioning

confidence: 99%

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Spin-trapping studies on the free-radical products formed by metabolic activation of carbon tetrachloride in rat liver microsomal fractions isolated hepatocytes and in vivo in the rat

Albano¹,

Lott²,

Slater³

et al. 1982

Biochemical Journal

168

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1. The metabolic activation of carbon tetrachloride to free-radical intermediates is an important step in the sequence of disturbances leading to the acute liver injury produced by this toxic agent. Electron-spin-resonance (e.s.r.) spin-trapping techniques were used to characterize the free-radical species involved. 2. Spin trapping was applied to the activation of carbon tetrachloride by liver microsomal fractions in the presence of NADPH, and by isolated intact rat hepatocytes. The results obtained with the spin trap N-benzylidene-2-methylpropylamine N-oxide ('phenyl t-butyl nitrone') (PBN) and [13Clcarbon tetrachloride provide unequivocal evidence for the formation and trapping of the trichloromethyl free radical in these systems. 3. With the spin trap 2-methyl-2-nitrosopropane, however, the major free-radical species trapped are unsaturated lipid radicals produced by the initiating reaction of lipid peroxidation. 4. Although pulse radiolysis and other evidence support the very rapid formation of the trichloromethyl peroxy radical from the trichloromethyl radical and oxygen, no clear evidence for the trapping of the peroxy radical was obtainable. 5. The effects of a number of free-radical scavengers and metabolic inhibitors on the formation of the PBN-trichloromethyl radical adduct were studied, as were the influences of changing the concentration of PBN and incubation time. 6. High concentrations of the spin traps used were found to have significant effects on cytochrome P-450-mediated reactions; this requires caution in interpreting results of experiments done in the presence of PBN at concentrations greater than 50mM.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Spin-trapping studies on the free-radical products formed by metabolic activation of carbon tetrachloride in rat liver microsomal fractions isolated hepatocytes and in vivo in the rat

Albano¹,

Lott²,

Slater³

et al. 1982

Biochemical Journal

168

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“…The first metabolite, a trichloromethyl free radical (.CCl 3 ) [21] has been formed from the metabolic conversion of CCl 4 and reacts very rapidly with O 2 and forms a second metabolite, a trichloromethyl peroxy free radical (CCl 3 OO) [22] or abstract hydrogen atoms to form chloroform. These free radicals initiate the peroxidation of membrane poly-unsaturated fatty acids and covalently bind to microsomal lipids and proteins [23].…”

Section: Discussionmentioning

confidence: 99%

Protective efficacy of natansnin, a dibenzoyl glycoside from Salvinia natans against CCl4 induced oxidative stress and cellular degeneration in rat liver

et al. 2010

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BackgroundCarbon tetra chloride (CCl4), an industrial solvent, is a hepatotoxic agent and it is the well established animal model for free radical-induced liver injury. The present investigation was carried out to establish the protective effect of natansnin, a novel dibenzoyl glycoside from Salvinia natans against CCl4 induced oxidative stress and cellular degeneration in rat liver.ResultsCCl4 significantly increased the levels of lipid peroxides, oxidized glutathione and decreased the levels of reduced glutathione, SOD and CAT. CCl4 induce marked histopathological changes and increase in the levels of apoptotic proteins. CCl4 treatment significantly increased the levels of apoptotic proteins such as caspases-3, PARP, Bax, Bid and cytochrome C and also increased the levels of inflammatory mediators iNos and Cox-2. Natansnin treatment significantly decreased the levels of CCl4 induced apoptotic proteins and inflammatory mediators. Further natansinin treatment significantly inhibited the CCl4 induced apoptosis which was evident form the reduced TUNEL positive cells.ConclusionsIn conclusion, our study demonstrated the protective effect of natansnin against CCl4 induced oxidative stress and cellular degeneration in rat liver tissue. This protective effect of natansnin can be correlated to its direct antioxidant effect.

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“…Paracetamol-induced hepatic injury is a commonly used experimental model for the screening of hepatoprotective drugs (38,39). The lesions produced are the result of the prooxidant effect of paracetamol, affecting blood parameters.…”

Section: Hepatoprotective Potentialmentioning

confidence: 99%

Hepatoprotective and antidiabetic activities of Fraxinus angustifolia Vahl extracts in animal models: characterization by high performance liquid chromatography analysis

et al. 2016

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Reactions of the carbon tetrachloride-related peroxy free radical (CCl3O2.) with amino acids : Pulse radiolysis evidence

Cited by 203 publications

References 4 publications

Spin-trapping studies on the free-radical products formed by metabolic activation of carbon tetrachloride in rat liver microsomal fractions isolated hepatocytes and in vivo in the rat

Spin-trapping studies on the free-radical products formed by metabolic activation of carbon tetrachloride in rat liver microsomal fractions isolated hepatocytes and in vivo in the rat

Protective efficacy of natansnin, a dibenzoyl glycoside from Salvinia natans against CCl4 induced oxidative stress and cellular degeneration in rat liver

Hepatoprotective and antidiabetic activities of Fraxinus angustifolia Vahl extracts in animal models: characterization by high performance liquid chromatography analysis

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