Reactivation of immobilized penicillin G acylase: Influence of cosolvents and catalytic modulators

Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization

“…However, it has been described in the scientific literature how the substrate and/or product participates in irreversible inactivation/activation mechanisms of enzymes that can alter the operational stability of the biocatalyst. 382,[496][497][498][499] Finally, as in many other biotechnological processes, the mixing and energy input across scales is of major importance. At small scale, the mixing is easily guaranteed, allowing control of the chemical reaction not only during the preparation of the catalyst but also during reaction analysis.…”

“…However, it has been described in the scientific literature how the substrate and/or product participates in irreversible inactivation/activation mechanisms of enzymes that can alter the operational stability of the biocatalyst. 382,496–499…”

Immobilization of Lipase B from Candida antarctica in Octyl-Vinyl Sulfone Agarose: Effect of the Enzyme-Support Interactions on Enzyme Activity, Specificity, Structure and Inactivation Pathway

“…The biocatalysts were vacuum filtered when their residual activity was 75%, 50% and 25%. All these biocatalysts were washed with 50 mM Tris buffer at pH 7, stored at 25 • C for 24 h to enable any reactivation [61,78,[87][88][89] and then stored at 4 • C to analyze their activities versus different substrates and fluorescence spectra.…”