Reactivation of inactivated endogenous proteolytic activities in phosphoric acid-etched dentine by etch-and-rinse adhesives

Mazzoni, Annalisa; Pashley, David H.; Nishitani, Yosuke; Breschi, Lorenzo; Mannello, Ferdinando; Tjäderhane, Leo; Toledano, Manuel; Pashley, Edna L.; Tay, Franklin R.

doi:10.1016/j.biomaterials.2006.01.040

Cited by 357 publications

(381 citation statements)

References 37 publications

(42 reference statements)

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“…The authors speculated bacterial colonization and the release of bacterial enzymes and of host-derived MMPs to have contributed to the degradation of collagen matrices in root dentin. The application of etch-and-rinse and self-etch adhesives in dentin bonding techniques have also been shown to activate collagenolytic and gelatinolytic processes mediated by the activation of endogenous MMPs in coronal dentin (65,66). Latent MMPs bound to dentin matrix are exposed following etching procedures and the low pH activates the enzymes by inducing conformational changes and exposing the catalytic domain (50).…”

Section: Ultrastructural Changes To Dentin Following Iatrogenic Procementioning

confidence: 99%

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Bioactive Chitosan Nanoparticles and Photodynamic Therapy Inhibit Collagen Degradation In Vitro

Persadmehr

Torneck

Cvitkovitch

et al. 2014

Journal of Endodontics

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This study evaluated the ability of photodynamic therapy (PDT), chitosan nanoparticles (CSnp), or their combination, to inhibit bacterial collagenase-mediated degradation of collagen. Rat type 1 fibrillar collagen matrices were untreated or treated with 2.5% glutaraldehyde (GD), 2.5% GD followed by 1% CSnp, 1% CSnp, PDT, or 1% CSnp followed by PDT. Samples, except untreated controls, were exposed to Clostridium histolyticum collagenase. The soluble digestion products were assessed by hydroxyproline assay and the remaining adherent collagen was quantified by picrosirius red (PSR) staining. Collagen treated with CSnp, PDT, or a combination of CSnp and PDT, exhibited less degradation than controls. The abundance of post-treatment residual collagen correlated with the extent of degradation. Fourier transform infrared (FTIR) spectroscopy analysis showed that PDT treatment enhanced collagen cross-linking.Immunoblotting of sedimented CSnp indicated that CSnp and collagenase bound with low affinity. However, CSnp-bound collagenase showed a significant reduction in collagenolytic activity compared with controls.III

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Section: Ultrastructural Changes To Dentin Following Iatrogenic Procementioning

confidence: 99%

“…In the absence of bacterial and salivary protease, residual collagenolytic activity was found in partially demineralized dentin powder from extracted, caries free teeth (65,66,122,123). CHX has been shown to inhibit MMP-2, -8, and -9 activities at low concentrations (124).…”

Section: Chlorhexidinementioning

confidence: 99%

Bioactive Chitosan Nanoparticles and Photodynamic Therapy Inhibit Collagen Degradation In Vitro

Persadmehr

Torneck

Cvitkovitch

et al. 2014

Journal of Endodontics

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“…Phosphoric acid etching can decrease MMP activity because of the low pH value (Pashley et al, 2004), but the simplified etch-and-rinse adhesives can reactivate the intrinsic MMPs trapped within dentin. This may be the reason why the degradation of hybrid layers takes place after using simplified etch-and-rinse adhesives in vivo and in vitro (Hebling et al, 2005;Mazzoni et al, 2006).…”

Section: Release and Activation Of Mmps And Their Potential Roles In mentioning

confidence: 99%

“…This degradation endangers the integrity of the interface created by infiltration of demineralized dentin substrate with adhesive resin monomers (Pashley et al, 2004;Mazzoni et al, 2006;Nishitani et al, 2006;Carrilho et al, 2007a;Breschi et al, 2008). In vitro, the release and activation of intrinsic proteinases have been confirmed to be responsible for the hydrolysis of dentin collagen within the hybrid layer owing to incomplete infiltration of resin adhesives (Pashley et al, 2004;Tay et al, 2006;Carrilho et al, 2007a).…”

Section: Release and Activation Of Mmps And Their Potential Roles In mentioning

confidence: 99%

“…The application of CHX to acid-etched human dentin can save the collagen fibrils in the hybrid layer when etch-and-rinse dentin bonding is used (King et al, 2005;Mazzoni et al, 2006;Brackett et al, 2009). This provides indirect proof of the involvement of MMPs in the degradation of collagen in the hybrid layer and the resulting decrease in bond strength over time.…”

Section: Chlorhexidinementioning

confidence: 99%

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The Role of Host‐derived Dentinal Matrix Metalloproteinases in Reducing Dentin Bonding of Resin Adhesives

2009

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Dentin matrix metalloproteinases (MMPs) are a family of host-derived proteolytic enzymes trapped within mineralized dentin matrix, which have the ability to hydrolyze the organic matrix of demineralized dentin. After bonding with resins to dentin there are usually some exposed collagen fibrils at the bottom of the hybrid layer owing to imperfect resin impregnation of the demineralized dentin matrix.Exposed collagen fibrils might be affected by MMPs inducing hydrolytic degradation, which might result in reduced bond strength.Most MMPs are synthesized and released from odontoblasts in the form of proenzymes, requiring activation to degrade extracellular matrix components. Unfortunately, they can be activated by modern self-etch and etch-and-rinse adhesives. The aim of this review is to summarize the current knowledge of the role of dentinal host-derived MMPs in dentin matrix degradation. We also discuss various available MMP inhibitors, especially chlorhexidine, and suggest that they could provide a potential pathway for inhibiting collagen degradation in bonding interfaces thereby increasing dentin bonding durability.

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Dental Adhesives

Derbanne¹,

Goff²,

Attal³

2014

Biomaterials

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Reactivation of inactivated endogenous proteolytic activities in phosphoric acid-etched dentine by etch-and-rinse adhesives

Cited by 357 publications

References 37 publications

Bioactive Chitosan Nanoparticles and Photodynamic Therapy Inhibit Collagen Degradation In Vitro

Bioactive Chitosan Nanoparticles and Photodynamic Therapy Inhibit Collagen Degradation In Vitro

The Role of Host‐derived Dentinal Matrix Metalloproteinases in Reducing Dentin Bonding of Resin Adhesives

Dental Adhesives

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