Reactive Arrays of Colorimetric Sensors for Metabolite and Steroid Identification

Abstract

The work described herein examines a rapid mix-and-measure method called DETECHIP suitable for screening of steroids and metabolites. The addition of steroids and metabolites to reactive arrays of colorimetric sensors generated characteristic color “fingerprints” that were used to identify the analyte. A color analysis tool was used to identify the analyte pool that now includes biologically relevant analytes. The mix-and-measure arrays allowed the detection of disease metabolites, orotic acid and argininosuccinic acid; and the steroids androsterone, 1,4-androstadiene, testosterone, stanozolol, and estrone. The steroid 1,4-androstadiene was also detected by this method while dissolved in synthetic urine. Some of the steroids, such as androstadiene, stanozolol, and androsterone were co-dissolved with (2-hydroxypropyl)-β-cyclodextrin in order to increase solubility in aqueous buffered solutions. The colorimetric arrays do not intend to eliminate ELISA or mass spectroscopy based screening, but to possibly provide an alternative analytical detection method for steroids and metabolites.

Comparative chemometric analysis for classification of acids and bases via a colorimetric sensor array

“…The array can be used to analyze many different substances, including narcotics, cutting agents, pesticides, steroids, and explosives using 8 sensors. 3,5,22,23 The first generation of the array involved simple visual analysis to determine whether a color change occurred for each sensor in the presence of an analyte. 3 An analyte-specific binary code was generated based on whether or not a color change occurred.…”

“…Our research group has studied extensively a colorimetric array that contains 8 sensors, many of which are acid‐base indicators. The array can be used to analyze many different substances, including narcotics, cutting agents, pesticides, steroids, and explosives using 8 sensors …”

An Improved Comparison of Chemometric Analyses for the Identification of Acids and Bases With Colorimetric Sensor Arrays

“…Colorimetric sensor arrays have been shown to detect a diverse range of analytes including ions, acids and bases, metal nanoparticles, explosives, pesticides, warfare agents, drugs, various organic compounds, complex mixtures (including coffee, beer, and soft drinks), and even biological molecules (steroids and proteins) (Askim et al, 2013; Bang, Lim, Park, & Suslick, 2008; Batres et al, 2014; Capitan-Vallvey et al, 2015; Chulvi et al, 2012; Johnke, Batres, Wilson, Holmes, & Sikich, 2013; Kitamura, Shabbir, & Anslyn, 2009; Lim, Feng, Kemling, Musto, & Suslick, 2009; Mahmoudi, Lohse, Murphy, & Suslick, 2016; Soga, Jimbo, Suzuki, & Citterio, 2013; Kangas, 2017). Versatile, colorimetric arrays have been used to detect analytes in solid, liquid, and gas phases (Feng, Musto, Kemling, Lim, & Suslick, 2010).…”

Spectrophotometric and chromatographic strategies for exploring of the nanostructure pharmaceutical formulations which contains testosterone undecanoate

“…13 (y = −0.012947, R 2 = 0.999875) for a series of ten reference solutions with a concentration of the compound of interest between 25-250 mg/L. Checking of Beer-Lambert relationship in the concentration domain, it enables the development of an analytical method for the determination of TU from the NPF 27,28 . www.nature.com/scientificreports www.nature.com/scientificreports/ Based on absorbance readings at 240.4 nm of samples diluted in absolute ethanol the analysis method has been developed and tested.…”