Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii

Yeşilada, Özfer; Birhanlı, Emre; Ercan, Sinem; Ozmen, Nesrin

doi:10.3906/biy-1308-38

Cited by 26 publications

(21 citation statements)

References 34 publications

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“…Up to 78% and 43% color removal were achieved by Coriolus versicolor and Pleurotus ostreatus respectively at the optimum enzyme activity (20U) after 120 minutes. This is in harmony to the findings of Yesilada et al (2014), who obtained maximum decolorization at highest enzyme activity. Similarly, Husain (2006) and Murugesan et al (2006) obtained similar results.…”

Section: Enzyme Effectsupporting

confidence: 91%

Enzymatic Decolorization of Remazol Brilliant Blue Royal (RB 19) textile dye by White Rot Fungi

Afiya¹,

Ahmet²,

Shah³

2019

jaar

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Synthetic dyes are widely used by different industries with over 7 ×10 5 metric tons produced globally each year. Dyes pose adverse effects including chemical oxygen demand, visual pollution, cytotoxicity, genotoxicity, mutagenicity and carcinogenicity on various types of living organisms. The versatile white rot fungi (basidiomycetes fungi) have developed specialized ligninolytic enzymes for reductive cleavage of dyes and xenobiotics. The present study optimized the decolorization of Remazol brilliant blue royal (RBBR) dye by enzymatic extracts of Coriolus versicolor and Pleurotus ostreatus. Experiments were carried out by varying one parameter i.e. pH (2.5-6.5), temperature (30 o C-60 o C), enzyme activity (3.3U-20U), dye concentration (10mg/L-125mg/L) and time (0-480mins), while others constant to study its effects on decolorization of RBBR. From the results obtained, the optimum conditions for decolorization of RBBR by extracts of C. versicolor and P. ostreatus were pH 4.0, temperature of 30 0 C, enzyme activity 20U, dye concentrations of 100mg/L and 50mg/L for C. versicolor and P. ostreatus respectively at the end of 480 minutes. At the optimized conditions, decolorizations for C. versicolor and P. ostreatus were 80.42% and 70.42% respectively. Highest laccase activity (19.50U) was recorded in C. versicolor compared to P. ostreatus (1.41U).

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Section: Enzyme Effectsupporting

confidence: 91%

Enzymatic Decolorization of Remazol Brilliant Blue Royal (RB 19) textile dye by White Rot Fungi

Afiya¹,

Ahmet²,

Shah³

2019

jaar

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“…In contrast, biological treatments present a relatively inexpensive, effective, and ecofriendly way to remove dyes from wastewaters Rauf and Salman Ashraf, 2012;Solís et al, 2012). In bioremediation/biodegradation processes, the metabolic potential of different microorganisms such as bacteria (Ölmezoglu et al, 2012;Gür et al, 2014), yeasts (Dönmez, 2002), fungi (Verma et al, 2010;Yeşilada et al, 2014), and algae (Khataee et al, 2010(Khataee et al, , 2011 are used to degrade a wide variety of pollutants.…”

Section: Introductionmentioning

confidence: 99%

Feasibility and assessment of the phytoremediation potential of duckweed for triarylmethane dye degradation with the emphasis on some physiologicalresponses and effect of operational parameters

Torbati¹

2015

Turk J Biol

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Phytoremediation is a low-cost and effective technology that removes pollutants, such as dye-containing effluents, from the environment through the use of plants. In the present study, the potential of Lemna minor L. for decolorization and degradation of the triarylmethane dye malachite green was investigated. The results revealed that the decolorization ability of the plant species is as high as 88%. The effect of some operational parameters (initial dye concentration, temperature, pH, and amount (weight) of plant) on the efficiency of biological decolorization process was determined. The metabolic fate of the dye was proposed by identifying 8 intermediate compounds produced during this process by gas chromatography-mass spectrometry. Some physiological responses of the plant were evaluated under 10 and 20 mg/L of the dye, with notable increases in superoxide dismutase and peroxidase activities at high concentrations. The results suggested that dye treatment induced oxidative stress and demonstrated duckweed's capacity to upregulate its antioxidative defense.

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“…It is possible to decolorize the dyes by using cell-free intracellular extract or extracellular culture filtrates of various organisms ( Yeşilada et al, 2014 a). Therefore, the decolorization activity of cell-free intracellular extract and culture filtrate was also tested.…”

Section: Resultsmentioning

confidence: 99%

“…Laccase (EC 1.10.3.2) activity was determined by measuring the increase in absorbance of ABTS [2,2′-Azinobis(3-ethylbenzthiazoline-6-sulfonic acid)] at 420 nm ( Yesilada et al, 2014 b). The assay mixture contained 100 mM sodium acetate buffer (pH 5.0), 0.5 mM ABTS, suitable amounts of cell-free culture filtrate and cell-free intracellular extract of the bacterium, and culture filtrate of F. trogii (Birhanli and Yesilada, 2006; Birhanli et al, 2013) .…”

Section: Methodsmentioning

confidence: 99%

Comparison of indigo carmine decolorization by Pseudomonas aeruginosa and crude laccase enzyme from Funalia trogii

et al. 2019

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The effects of incubation time, temperature, initial pH, and dye concentration on the indigo carmine decolorization activity of Pseudomonas aeruginosa ATCC 10145 and some factors on the decolorization potential of crude laccase enzyme obtained from Funalia trogii ATCC 200800 were comparatively investigated. This bacterium showed effective decolorization activity at all agitation and temperature values. Indigo carmine was greatly decolorized by P. aeruginosa at all pH values except pH 10. A decrease in decolorization activity occurred with increasing dye concentration, but this bacterium effectively decolorized the dye within 24 h. The decolorization process was through microbial metabolism, not biosorption. No decolorization or laccase activity could be obtained by the cell-free intracellular extract or culture filtrate of this bacterium. On the other hand, crude laccase effectively decolorized indigo carmine under highly acidic conditions, especially at pH 3.0 as 57% in 300 seconds. This activity decreased progressively due to the increase in pH values. In a short incubation period and at high temperature values, the crude laccase enzyme removed the color of the dye at 50 °C (56%), 60 °C (45%), and 70 °C (38%). These data are important for improving methods for decolorization of textile dyes used at high temperatures in various industrial applications.

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Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii

Cited by 26 publications

References 34 publications

Enzymatic Decolorization of Remazol Brilliant Blue Royal (RB 19) textile dye by White Rot Fungi

Enzymatic Decolorization of Remazol Brilliant Blue Royal (RB 19) textile dye by White Rot Fungi

Feasibility and assessment of the phytoremediation potential of duckweed for triarylmethane dye degradation with the emphasis on some physiologicalresponses and effect of operational parameters

Comparison of indigo carmine decolorization by <i>Pseudomonas</i> <i>aeruginosa</i> and crude laccase enzyme from <i>Funalia</i> <i>trogii</i>

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