Reactive oxygen: A novel antimicrobial mechanism for targeting biofilm-associated infection

Dryden, Matthew; Cooke, Jonathan; Salib, Rami J.; Holding, Rebecca; Biggs, Timothy C.; Salamat, Ali; Allan, Raymond N.; Newby, Rachel S.; Halstead, Fenella D.; Oppenheim, Beryl; Hall, Thomas J.; Cox, Sophie C.; Grover, Liam M.; Al-hindi, Zain; Frazer, Lilyann Novak; Richardson, Malcolm

doi:10.1016/j.jgar.2016.12.006

Cited by 41 publications

(30 citation statements)

References 26 publications

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“…In recent years, multiple reports indicated rapid development of antibiotic resistance by S. aureus [31], which further speeds up in biofilms [2,32]. To identify the intracellular targets of F105 , we attempted to obtain a resistant strain.…”

Section: Discussionmentioning

confidence: 99%

“…To conclude, F105 might penetrate the cells and induce oxidative stress, making resistance development highly unlikely. Current development of ROS delivery/induction methods that would be free of side effects for the host tissues represent a promising approach for the topical treatment of infections caused by drug-resistant pathogens [32]. Accordingly, we believe that F105 is capable of intracellular ROS induction activation and is a promising candidate as a ROS inducing antibacterial agent.…”

Section: Discussionmentioning

confidence: 99%

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Unraveling the Molecular Mechanism of Selective Antimicrobial Activity of 2(5H)-Furanone Derivative against Staphylococcus aureus

Sharafutdinov

Pavlova

Akhatova

et al. 2019

IJMS

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Staphylococcus aureus causes various infectious diseases, from skin impetigo to life-threatening bacteremia and sepsis, thus appearing an important target for antimicrobial therapeutics. In turn, the rapid development of antibiotic resistance and biofilm formation makes it extremely robust against treatment. Here, we unravel the molecular mechanism of the antimicrobial activity of the recently unveiled F105 consisting of three pharmacophores: chlorinated 2(5H)-furanone, sulfone, and l-menthol moieties. F105 demonstrates highly selective activity against Gram-positive bacteria and biofilm-embedded S. aureus and exhibits low risk of resistance development. We show explicitly that the fluorescent analogue of F105 rapidly penetrates into Gram-positive bacteria independently of their cell integrity and viability and accumulates there. By contrast, Gram-negative bacteria remain impermeable and, therefore, insusceptible to F105. Apparently, in bacterial cells, F105 induces reactive oxygen species (ROS) formation and nonspecifically interacts with a number of proteins, including ROS-utilizing ones. Using native and 2D PAGE, we confirm that F105 changes the charge of some proteins by either oxidation or direct interaction with them. Therefore, it seems justified to conclude that being simultaneously a ROS inducer and damaging proteins responsible for ROS utilization, F105 impairs the cellular anti-ROS defense representing a prospective ROS-inducing antibacterial agent.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Unraveling the Molecular Mechanism of Selective Antimicrobial Activity of 2(5H)-Furanone Derivative against Staphylococcus aureus

Sharafutdinov

Pavlova

Akhatova

et al. 2019

IJMS

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“…Many antimicrobial agents have been shown to reduce the bacterial load in wounds and hence play an important role in wound management strategies . However, there is some evidence to suggest that topical antimicrobial agents may also be cytotoxic to host cells .…”

Section: Discussionmentioning

confidence: 99%

The wound‐healing effects of a next‐generation anti‐biofilm silver Hydrofiber wound dressing on deep partial‐thickness wounds using a porcine model

Davis

Gil

et al. 2018

International Wound Journal

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Topical antimicrobials are widely used to control wound bioburden and facilitate wound healing; however, the fine balance between antimicrobial efficacy and non-toxicity must be achieved. This study evaluated whether an anti-biofilm silver-containing wound dressing interfered with the normal healing process in non-contaminated deep partial thickness wounds. In an in-vivo porcine wound model using 2 pigs, 96 wounds were randomly assigned to 1 of 3 dressing groups: anti-biofilm silver Hydrofiber dressing (test), silver Hydrofiber dressing (control), or polyurethane film dressing (control). Wounds were investigated for 8 days, and wound biopsies (n = 4) were taken from each dressing group, per animal, on days 2, 4, 6, and 8 after wounding and evaluated using light microscopy. No statistically significant differences were observed in the rate of reepithelialisation, white blood cell infiltration, angiogenesis, or granulation tissue formation following application of the anti-biofilm silver Hydrofiber dressing versus the 2 control dressings. Overall, epithelial thickness was similar between groups. Some differences in infiltration of specific cell types were observed between groups. There were no signs of tissue necrosis, fibrosis, or fatty infiltration in any group. An anti-biofilm silver Hydrofiber wound dressing did not cause any notable interference with normal healing processes.

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“… 5 , 6 The use of ROS is a new therapeutic approach for topical use on skin, mucosal membranes, or internal tissue that may be colonized with microbial inhabitants and biofilms. 7 This study presents an overview of ROS formation and the use of ROS-dependent procedures for antimicrobial therapy.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial use of reactive oxygen therapy: current insights

Memar¹,

Ghotaslou²,

Samiei³

et al. 2018

IDR

186

126

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Infections caused by drug-resistant pathogens are a global public health problem. The introduction of a new antimicrobial strategy is an unavoidable option for the management of drug-resistant pathogens. Induction of high levels of reactive oxygen species (ROS) by several procedures has been extensively studied for the treatment of infections. In this article, the general aspects of ROS production and the common procedures that exert their antimicrobial effects due to ROS formation are reviewed. ROS generation is the antimicrobial mechanism of nanoparticles, hyperbaric oxygen therapy, medical honey, and photodynamic therapy. In addition, it is an alternative bactericidal mechanism of clinically traditional antibiotics. The development of ROS delivery methods with a desirable selectivity for pathogens without side effects for the host tissue may be a promising approach for the treatment of infections, especially those caused by drug-resistant organisms.

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Reactive oxygen: A novel antimicrobial mechanism for targeting biofilm-associated infection

Cited by 41 publications

References 26 publications

Unraveling the Molecular Mechanism of Selective Antimicrobial Activity of 2(5H)-Furanone Derivative against Staphylococcus aureus

Unraveling the Molecular Mechanism of Selective Antimicrobial Activity of 2(5H)-Furanone Derivative against Staphylococcus aureus

The wound‐healing effects of a next‐generation anti‐biofilm silver Hydrofiber wound dressing on deep partial‐thickness wounds using a porcine model

Antimicrobial use of reactive oxygen therapy: current insights

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