Reactive Oxygen Species and Lipid Peroxidation Product-Scavenging Ability of Yogurt Organisms

Lin, Meei‐Yn; Yen, Chyuan-Liang

doi:10.3168/jds.s0022-0302(99)75391-9

Cited by 57 publications

(34 citation statements)

References 19 publications

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“…In this work, the in vitro antioxidant activity of the food isolated enterococci was observed by different methods, although the radical scavenging effect was only observed for culture supernatants. On contrast, free radical scavenging activity was described to intracellular extracts of some LAB, such as Lactobacillus delbrueckii (Lin and Yen, 1999), Lactobacillus acidophilus and Bifidobacterium longum (Lin and Chang, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…The antioxidant activity of some species of LAB has been demonstrated by in vitro enzymatic assays (Lin and Yen, 1999). The capacity to accumulate selenite (Se 4+ ), an essential element showing antioxidant properties (Thirunavukkarasu et al, 2004) has been described for some isolates of Enterococcus (Pieniz et al, 2011) and other LAB (Svoboda et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial and antioxidant activities of Enterococcus species isolated from meat and dairy products

et al. 2015

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Lactic acid bacteria (LAB) have an important role in a great variety of fermented foods. In addition to their contribution to sensory characteristics, they enhance food preservation and can be used as probiotics. In this study, the antimicrobial and antioxidant activities of culture supernatants and cell free extracts of 16 LAB isolated from meat and dairy products were investigated. The bacterial were identified by 16S rRNA sequencing. GenBank BLAST analysis revealed that all the isolates belong to Enterococcus faecium species. Antimicrobial activity against the indicator microorganism (Listeria monocytogenes) was observed at 11 culture supernatants and 4 cell free extracts. The sensibility of culture supernatant was evaluated by proteinase K and trypsin and it was observed that activity of antimicrobial substance was completely lost after the treatment. All of the isolates showed antioxidant activity as determined by the Thiobarbituric Acid Reactive Substances (TBARS) method with both types of extracts. When the antioxidant capacity was investigated using ABTS •+ method (2,2 azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) and DPPH method (2,2-diphenyl-1-picrylhydrazyl) it was observed that only culture supernatants showed antioxidant capacity. These bacteria could particularly help to reduce or inhibit pathogenic microorganisms as well as oxidative spoilage in foods and feed.Keywords: antimicrobial activity, antioxidant, Enterococcus, lactic acid bacteria, molecular characterization.Atividade antimicrobiana e antioxidante de espécies de Enterococcus isoladas de carne e produtos lácteos ResumoAs bactérias ácido láticas (BAL) têm um papel importante em uma grande variedade de alimentos fermentados. Em adição à sua contribuição para as características sensoriais, estes microorganismos melhoram a conservação de alimentos e podem ser utilizados como probióticos. Neste estudo, as atividades antimicrobiana e antioxidante do sobrenadante e dos extratos livres de células de 16 isolados de LAB de carne e produtos lácteos foram investigadas. Os isolados foram identificados pelo sequenciamento da região 16S do rRNA. Após a comparação das sequências obtidas com aquelas disponíveis na base de dados GenBank, observou-e que todos os isolados foram pertencentes à espécie Enterococcus faecium. A atividade antimicrobiana contra o microrganismo indicador (Listeria monocytogenes) foi observada no sobrenadante das culturas em 11 isolados, e nos extratos livres de células por 4 isolados. A sensibilidade da cultura sobrenadante foi avaliada pela proteinase K e tripsina e observou-se que a atividade da substância antimicrobiana foi completamente perdida após o tratamento com as enzimas proteolíticas. Todos os isolados apresentaram atividade antioxidante, como determinado pelo método do ácido tiobarbitúrico de substâncias reativas (TBARS) com ambos os tipos de extratos. Quando a capacidade antioxidante foi investigada usando o método do ABTS (2,2 azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) e o método de DPPH (2,2-di...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antimicrobial and antioxidant activities of Enterococcus species isolated from meat and dairy products

et al. 2015

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“…A few in vitro studies have reported that these bacteria or their extracts show a scavenging ability for lipid peroxidation products (malondialdehyde [MDA]) and also that they are able to protect plasma against lipid peroxidation (27)(28)(29).…”

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confidence: 99%

Differential Effects of Bifidobacterium pseudolongum Strain Patronus and Metronidazole in the Rat Gut

Vasquez

Suau

Magne

et al. 2009

Appl Environ Microbiol

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In the luminal contents of metronidazole-treated rats, there was a dominant Bifidobacterium species. A strain has been isolated, its 16S rRNA gene has been sequenced, and the strain has been named Bifidobacterium pseudolongum strain Patronus. In this study, using an experimental model of healthy rats, the effects of metronidazole treatment and B. pseudolongum strain Patronus administration on the luminal and mucosaassociated microbiota and on gut oxidation processes were investigated. Metronidazole treatment and the daily gavage of rats with B. pseudolongum strain Patronus increased the numbers of bifidobacteria in cecal contents and in cecal mucosa-associated microbiota compared with those in control rats. Metronidazole reduced the colonic oxidative damage to proteins. This is the first evidence that B. pseudolongum strain Patronus exerts an effect on a biomarker of oxidative damage by reducing the susceptibility to oxidation of proteins in the colon and the small bowel. Antioxidant effects of metronidazole could be linked to the bifidobacterial increase but also to other bacterial modifications.

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“…The anti-oxidative effect of lactic acid bacteria has been reported [18]. Both of the bacterial strains used in this study, S. thermophilus 1131 and L. bulgaricus 2038, showed production of radical scavengers and inhibited oxidation of erythrocyte membranes and low-density lipoprotein in vitro [40].…”

Section: Discussionmentioning

confidence: 96%

Inhibitory Effect of Yogurt on Aberrant Crypt Foci Formation in the Rat Colon and Colorectal Tumorigenesis in RasH2 Mice

et al. 2010

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Abstract:The inhibitory effects of yogurt consisting of milk fermented by Lactobacillus delbrueckii subsp. bulgaricus strain 2038 and Streptococcus salivarius subsp. thermophilus strain 1131 on formation of colonic aberrant crypt foci (ACF) in rats and also on development of colorectal tumors in transgenic mice harboring human prototype c-Ha-ras genes (rasH2 mice) were examined. F344 rats and rasH2 mice were fed commercial diet containing freezedried yogurt or starter medium (non-fermented milk). Rats were inoculated orally with heterocyclic amine 2-amino-methyl-6-phenylimidazo [4,5-b]pyridine hydrochloride (PhIP) for two weeks. The rats were necropsied 14 days after the PhIP treatment, and ACF in the colon and rectum were counted. RasH2 mice were injected with 1,2-dimethylhydrazine dihydrochloride (DMH) for 20 weeks. Three weeks after the last injection of DMH, rasH2 mice were necropsied to determine the number and the size of colorectal tumors. Yogurt supplementation in diet significantly reduced the number of ACF and aberrant crypts (ACs) in rats fed control diet (P<0.01), but not in rats fed non-fermented milk diet. On the other hand, rasH2 mice receiving the yogurt-supplemented diet had significantly reduced numbers of tumors induced by DMH compared with those fed the non-fermented milk-supplemented diet (P<0.05). These results demonstrate that the yogurt used in this study appears to have tumor-suppressing properties, and rasH2 mice are a useful model for the evaluation of antitumor activities of foods.

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Reactive Oxygen Species and Lipid Peroxidation Product-Scavenging Ability of Yogurt Organisms

Cited by 57 publications

References 19 publications

Antimicrobial and antioxidant activities of Enterococcus species isolated from meat and dairy products

Antimicrobial and antioxidant activities of Enterococcus species isolated from meat and dairy products

Differential Effects of Bifidobacterium pseudolongum Strain Patronus and Metronidazole in the Rat Gut

Inhibitory Effect of Yogurt on Aberrant Crypt Foci Formation in the Rat Colon and Colorectal Tumorigenesis in RasH2 Mice

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