Reactive oxygen species generation by human spermatozoa is induced by exogenous NADPH and inhibited by the flavoprotein inhibitors diphenylene iodonium and quinacrine

Aitken, R. John; Fisher, Helen M.; Fulton, Norma; Gómez, E.; Knox, W. Eugene; Lewis, Beverley; Irvine, Stewart

doi:10.1002/(sici)1098-2795(199708)47:4<468::aid-mrd14>3.0.co;2-s

Cited by 274 publications

(92 citation statements)

References 53 publications

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“…Moreover, ROS also damage sperm nuclear DNA, displaying negative effects on sperm concentration and morphology and causing an increased risk of DNA fragmentation. 28 40 In line with these findings, our data showed significantly higher ROS levels in azoospermic men, as well as reduced TAC.…”

Section: Discussionsupporting

confidence: 82%

Redox status assessment in infertile patients with non‐obstructive azoospermia undergoing testicular sperm extraction: A prospective study

et al. 2019

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Background Oxidative stress (OS) is one of the most prevalent causes of sperm damage, through the toxic effects of endogenously generated hydrogen peroxide, superoxide anion, and hydroxyl radicals. Peripheral leukocytes represent a feasible model for studying the pathophysiology of OS‐mediated homeostasis, which can be responsible for cell dysfunction and cell injury. Objective To evaluate the redox status in patients with non‐obstructive azoospermia (NOA), establishing the potential role exerted by reactive oxygen species (ROS) in the genesis of testicular secretory injury. Material and methods From May 2018 to March 2019, 39 patients were enrolled in this prospective single‐center cohort study and divided into two groups. Group 1 included 19 patients with NOA, and Group 2 included 20 normozoospermic men, partners of women with infertility tubal factor. All patients underwent serum blood tests. NOA underwent testicular sperm extraction (TeSE). ROS production (in lymphocytes, monocytes, and granulocytes) was assessed by fluorescence‐activated cell sorting (FACS) analysis. Plasma oxidative stress was evaluated by lipid peroxidation markers (MDA) and total antioxidant capacity (TAC) both assessed by fluorometric techniques. Results Mean lymphocyte ROS production resulted 967.0 ± 224.5 vs 728.0 ± 98.0 (NOA vs Controls, P < .001), monocyte ROS resulted 2102.5 ± 517.5 vs 1253 ± 171 (P < .001), and granulocyte ROS were 2366.5 ± 595.4 vs 1751.0 ± 213.0 (P < .001). Significant increases plasma lipid peroxidation markers were found in NOA patients compared with controls (2.7 ± 0.8 vs 0.37 ± 0.2 nmol/mL, P < .001). Significant decreased TAC was evident in NOA compared with controls (13.4 ± 3.9 vs 3.0 ± 0.2 µmol/mL Trolox equivalents, P < .001). No significant differences were found in blood leukocyte subpopulations ROS production, plasma lipid peroxidation, and TAC comparing groups (positive vs negative sperm retrieval, P > .05). Conclusion ROS production can be directly related to disorders of spermatogenesis, leading to severe conditions of male infertility, including azoospermia.

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Section: Discussionsupporting

confidence: 82%

Redox status assessment in infertile patients with non‐obstructive azoospermia undergoing testicular sperm extraction: A prospective study

et al. 2019

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“…Aitken (1997) suggested a significant positive correlation between retention of cytoplasmic droplets by spermatozoa and ROS generation through a mechanism that may be assisted by the cytosolic enzyme, glucose-6-phosphate dehydrogenase [21]. Two enzyme systems have been proposed to be responsible for ROS production.…”

Section: Origin Of Rosmentioning

confidence: 99%

Male Infertility: The Effect of Natural Antioxidants and Phytocompounds on Seminal Oxidative Stress

et al. 2017

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Defective sperm function has been identified as the most common cause of infertility. The objective of this study was to review recent findings on the effects of various antioxidants on male fertility. High amounts of poly unsaturated fatty acid are found in the mammalian spermatozoa membranes, thereby making them susceptible to lipid peroxidation. Although, free radicals and reactive oxygen species (ROS) play major roles in reproduction, they are strongly associated with oxidative stress. Furthermore, factors such as obesity, inflammation, pollutants and cigarette smoking are negatively correlated with spermatogenesis. Endogenous antioxidants system exists to mediate these damages. In a normal physiological state, the seminal plasma contains antioxidant enzyme mechanism that is capable of quenching these ROS as well as protecting the spermatozoa against any likely damage. However, high level of ROS triggered by inflammatory cells and oxidation of fatty acid in obese subjects may down play antioxidant mechanism resulting in oxidative stress. Evaluation of such oxidative stress is the first step in the treatment of male infertility through administration of suitable antioxidant. Notably, antioxidant such as vitamin E and C, carotenoids and carnitine have been found beneficial in restoring a balance between ROS generation and scavenging activities. There are emerging evidences that herbal products can also boost male reproductive functions. Nonetheless, a good lifestyle, regular exercise, avoidance of stress and observing safety rules at work are habits that can reverse male infertility.

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“…Of these, leukocytes are considered to be the primary source (Aitken et al, 1992). Leukocytes, particularly neutrophils and macrophages, have been associated with excessive ROS production that ultimately leads to sperm dysfunction (Aitken and Baker, 1995; Aitken et al, 1997; Hendin et al, 1999; Ochsendorf, 1999; Pasqualotto et al, 2000; Saleh et al, 2002; Shalika et al, 1996; Sharma et al, 2001).…”

Section: Sources Of Ros In Semenmentioning

confidence: 99%

Prevention of Oxidative Stress Injury to Sperm

Agarwal

Prabakaran

Said

2005

Journal of Andrology

254

134

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Reactive oxygen species generation by human spermatozoa is induced by exogenous NADPH and inhibited by the flavoprotein inhibitors diphenylene iodonium and quinacrine

Cited by 274 publications

References 53 publications

Redox status assessment in infertile patients with non‐obstructive azoospermia undergoing testicular sperm extraction: A prospective study

Redox status assessment in infertile patients with non‐obstructive azoospermia undergoing testicular sperm extraction: A prospective study

Male Infertility: The Effect of Natural Antioxidants and Phytocompounds on Seminal Oxidative Stress

Prevention of Oxidative Stress Injury to Sperm

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