Reactive oxygen species impact on sperm DNA and its role in male infertility

Bui, Albert; Sharma, Rakesh K.; Henkel, Ralf; Agarwal, Ashok

doi:10.1111/and.13012

Cited by 227 publications

(159 citation statements)

References 105 publications

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“…It has been previously described that oxidative stress increases DNA fragmentation (Bui, Sharma, Henkel, & Agarwal, ), being more evident during the cryopreservation of stallion sperm (Treulen et al, ). The results obtained in the present study confirm that the antioxidants added to the freezing medium do not compromise the integrity of the sperm DNA.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of stallion semen: Effect of adding antioxidants to the freezing medium on sperm physiology

Contreras

Treulén

Arias

et al. 2020

Reprod Domestic Animals

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Cryopreservation of stallion semen has not reached the level of efficiency and positive results described in other species. This is mainly due to the greater sensitivity of stallion sperm to the freezing process, showing higher rates of oxidative stress and plasma membrane damage, which trigger the activation of several cell damage pathways that ultimately culminate in DNA fragmentation and cell death. Therefore, finding molecules that improve the efficiency of this technique in stallion by preventing oxidative stress and cell damage is required. Thus, the aim of the present study was to evaluate the effect of adding three antioxidants (MnTBAP, NAC and FeTPPS) to the freezing medium on the quality and functional parameters of stallion sperm. Semen samples from three stallions frozen with the antioxidants were evaluated in two conditions: (a) adding the antioxidants before freezing, and (b) before and after freezing. Plasma membrane integrity, mitochondrial membrane potential, lipid peroxidation, intracellular ROS levels, membrane lipid disorder, DNA damage, sperm motility and binding to the zona pellucida were assessed. The results showed that MnTBAP was the antioxidant treatment that best controlled the oxidative stress process and post‐thaw cell damage, showing higher plasma membrane integrity, mitochondrial membrane potential, sperm motility, number of spermatozoa bound to the zona pellucida of bovine oocytes and lower lipid disorder. Additionally, it was determined that a second post‐thaw application of antioxidants is detrimental since induced higher cell damage and lower sperm motility, without showing any beneficial effect on the spermatozoa.

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Section: Discussionmentioning

confidence: 99%

Cryopreservation of stallion semen: Effect of adding antioxidants to the freezing medium on sperm physiology

Contreras

Treulén

Arias

et al. 2020

Reprod Domestic Animals

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“…ROS is generated by sperm mitochondria, which are a considerable source of ROS, in the form of cytoplasmic droplets (excess of residual cytoplasm) in spermatozoa. Pathological conditions such as leukocytospermia, varicocele, genitourinary tract infections and chronic inflammations induce ROS production and contribute to sperm immobility (Aitken, Gibb, Baker, Drevet, & Gharagozloo, 2016;Bui, Sharma, Henkel, & Agarwal, 2018 Ghanem, Shaeer, and El-Segini (2010) iOAT individuals were randomized into equally three groups (N = 90)…”

Section: Sperm Motility Molecular Pathways and Lifestylementioning

confidence: 99%

Asthenozoospermia: Cellular and molecular contributing factors and treatment strategies

et al. 2019

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| INTRODUC TI ONIn recent years, many researchers have focused on possible factors leading to male infertility and revealed the existence of many cellular and molecular defects during sperm production and maturation. These defects finally affect the count and structure of spermatozoa and reduce the ability of spermatozoa to reach and fertilise the egg. A decrease in sperm motility, called "asthenozoospermia," is one of these important deficiencies, which is influenced by various cellular and molecular factors. After a brief explanation of the main cellular and molecular factors involved in reducing sperm motility, this review article presents some important treatment strategies to overcome sperm motility reduction. | DEFINITI ON AND PRE VALEN CEAccording to the reports released by the World Health Organization (WHO), about 20% of couples are infertile and infertility can be, at least partly, attributed to the male partner (Petok, 2015). Low sperm count, abnormal morphology and poor sperm motility are the common causes related to male factors. Based on the WHO guideline (2010), asthenozoospermia was defined as total motility <40% and progressive motility <32% in a semen sample (WHO, 2010).Severe asthenozoospermia was also characterised by total sperm immobility or very low motile spermatozoa in the semen sample. It seems that total sperm immobility is related to genetic disorders (Blouin et al., 2000). This will be explained in more detail below.Understating the cellular and molecular processes leading to sperm motility is required to help the researchers to address the subject of reduced sperm motility and produce an accurate diagnosis (Turner, 2003).To diagnose asthenozoospermia in individuals in an andrology laboratory, some important points must be taken into consideration to prevent false asthenozoospermia diagnosis. For instance, semen should not be collected in long abstinence days since the number of immotile spermatozoa is high in abstinence days. Also, semen AbstractSemen sample with poor sperm motility, which called asthenozoospermia, is considered as one of the main factors contributing to male infertility. Recognition of the cellular and molecular pathways contributing to sperm motility reduction may lead to applying novel treatment strategies for overcoming low sperm motility in asthenozoospermia individuals. In this review, we intend to discuss the main causes of sperm motility reduction in asthenozoospermia and some treatment strategies used to overcome low sperm motility. K E Y W O R D Sapoptosis, reactive oxygen species, spermiogenesis

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“…At physiological concentrations, ROS occupy an essential role in mammal sperm capacitation (Aitken, 2006). However, a disproportionate increase in ROS or decrease in antioxidant barriers can disrupt the equilibrium, producing a redox imbalance or oxidative stress, which generates damage as lipid peroxidation of the membranes or DNA damage of the spermatozoa (Aitken, 2006;Bui, Sharma, Henkel, & Agarwal, 2018;Lopes, Jurisicova, Sun, & Casper, 1998;Pereira, Sá, Barros, & Sousa, 2015;Walczak-Jedrzejowska, Wolski, & Slowikowska-Hilczer, 2013). A high level of ROS is strongly associated with human male factor infertility (Agarwal et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…In human, an overproduction of ROS is due to immature spermatozoa and leucocytes in the seminal plasma, and their number can increase with inflammation, infection and cellular defense mechanisms (Henkel, 2011). A great number of factors can contribute to increase oxidative stress, such as genitourinary infections, varicocele, stress, and smoking among others (Bui et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Hydrogen peroxide (H 2 O 2 ) is one of the compounds of ROS that is made in sperm. Oxidative stress produces DNA damage, including DNA fragmentation, mitochondrial DNA damage, epigenetic abnormalities, telomere attrition, and Y chromosome microdeletions (Bui et al, 2018). The incubation of sperm in low H 2 O 2 concentrations was found to significantly increase damage of zebrafish (Danio rerio) sperm DNA (Reinardy, Skippins, Henry, & Jha, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Effect of different oxidative stress degrees generated by hydrogen peroxide on motility and DNA fragmentation of zebrafish (Danio rerio) spermatozoa

Sadeghi

Pertusa

Yániz

et al. 2018

Reprod Domestic Animals

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An increase in reactive oxygen species (ROS) or decrease in antioxidant barriers can provoke lipid peroxidation of the membranes or DNA damage of the spermatozoa. The aim of this work is to study the effect of the different degrees of oxidative stress generated by H O incubation on total motility, kinetics, and DNA fragmentation of zebrafish (Danio rerio) spermatozoa. For this process, experimental groups were incubated in 50 µM (Low; L) and 200 µM (High; H) H O , respectively, for 20 min at 4ºC. Sperm motility parameters were obtained with a computer-assisted sperm analysis (CASA) system. Sperm DNA fragmentation (SDF) was assessed using the sperm chromatin dispersion test. Both low and high H O concentration groups showed lower motility than control groups. Progressive motility of spermatozoa incubated in the H group dropped rapidly in comparison with other groups. Regarding SDF, the control and L groups had significantly lower values than the H group (25.0% and 31.6% vs. 48.1% fragmented sperm for C, L, and H groups, respectively; p < 0.05). Sperm motility, mostly progressive motility, decreased as H O concentration increased, mainly when time after sperm activation increased. SDF increased as the H O concentration increased. However, measurements of the halo area did not agree with the subjective SDF rate.

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Reactive oxygen species impact on sperm DNA and its role in male infertility

Cited by 227 publications

References 105 publications

Cryopreservation of stallion semen: Effect of adding antioxidants to the freezing medium on sperm physiology

Cryopreservation of stallion semen: Effect of adding antioxidants to the freezing medium on sperm physiology

Asthenozoospermia: Cellular and molecular contributing factors and treatment strategies

Effect of different oxidative stress degrees generated by hydrogen peroxide on motility and DNA fragmentation of zebrafish (Danio rerio) spermatozoa

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