Reactive oxygen species in photochemistry of the red fluorescent protein “Killer Red”

Vegh, Russell B.; Solntsev, Kyril M.; Kuimova, Marina K.; Cho, Soohee; Liang, Yue; Loo, Bernard L. W.; Tolbert, Laren M.; Bommarius, Andreas S.

doi:10.1039/c0cc05713d

Cited by 105 publications

(134 citation statements)

References 30 publications

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“…In this context, it is important to bear in mind that conflicting data have been reported regarding the types of ROS that are produced by KR: whereas Bulina and co-workers reported that the primary damaging agent generated by KR is singlet oxygen ( 1 O 2 ) [23], others claim that the cytotoxic effects of this photosensitizer are primarily mediated by other ROS such as superoxide (O 2 d À ) and hydrogen peroxide (H 2 O 2 ) [46,47].…”

Section: Prevention Of Po-kr-induced Cell Death By Chemical and Enzymmentioning

confidence: 97%

Mitochondria are targets for peroxisome-derived oxidative stress in cultured mammalian cells

Wang

Veldhoven

Brees

et al. 2013

Free Radical Biology and Medicine

125

109

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Many cellular processes are driven by spatially and temporally regulated redox-dependent signaling events. Although mounting evidence indicates that organelles such as the endoplasmic reticulum and mitochondria can function as signaling platforms for oxidative stress-regulated pathways, little is known about the role of peroxisomes in these processes. In this study, we employ targeted variants of the genetically encoded photosensitizer KillerRed to gain a better insight into the interplay between peroxisomes and cellular oxidative stress. We show that the phototoxic effects of peroxisomal KillerRed induce mitochondria-mediated cell death and that this process can be counteracted by targeted overexpression of a select set of antioxidant enzymes, including peroxisomal glutathione S-transferase kappa 1, superoxide dismutase 1, and mitochondrial catalase. We also present evidence that peroxisomal disease cell lines deficient in plasmalogen biosynthesis or peroxisome assembly are more sensitive to KillerRed-induced oxidative stress than control cells. Collectively, these findings confirm and extend previous observations suggesting that disturbances in peroxisomal redox control and metabolism can sensitize cells to oxidative stress. In addition, they lend strong support to the ideas that peroxisomes and mitochondria share a redox-sensitive relationship and that the redox communication between these organelles is not only mediated by diffusion of reactive oxygen species from one compartment to the other. Finally, these findings indicate that mitochondria may act as dynamic receivers, integrators, and transmitters of peroxisome-derived mediators of oxidative stress, and this may have profound implications for our views on cellular aging and age-related diseases.

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Section: Prevention Of Po-kr-induced Cell Death By Chemical and Enzymmentioning

confidence: 97%

Mitochondria are targets for peroxisome-derived oxidative stress in cultured mammalian cells

Wang

Veldhoven

Brees

et al. 2013

Free Radical Biology and Medicine

125

109

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“…The phototoxic protein KillerRed, developed from a red chromoprotein homolog of GFP, has been used to kill cultured cells when targeted to mitochondria, membranes, or histones and illuminated with green light (12,38). KillerRed works through generation of radicals or hydrogen peroxide rather than through singlet oxygen (15,39). miniSOG has an additional advantage for tagging functional proteins because it is a monomer less than one quarter the size of the obligate dimer of KillerRed (12,15).…”

Section: Discussionmentioning

confidence: 99%

Photo-inducible cell ablation in Caenorhabditis elegans using the genetically encoded singlet oxygen generating protein miniSOG

Garren

Shu

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

200

206

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We describe a method for light-inducible and tissue-selective cell ablation using a genetically encoded photosensitizer, miniSOG (mini singlet oxygen generator). miniSOG is a newly engineered fluorescent protein of 106 amino acids that generates singlet oxygen in quantum yield upon blue-light illumination. We transgenically expressed mitochondrially targeted miniSOG (mito-miniSOG) in Caenorhabditis elegans neurons. Upon blue-light illumination, mitominiSOG causes rapid and effective death of neurons in a cell-autonomous manner without detectable damages to surrounding tissues. Neuronal death induced by mito-miniSOG appears to be independent of the caspase CED-3, but the clearance of the damaged cells partially depends on the phagocytic receptor CED-1, a homolog of human CD91. We show that neurons can be killed at different developmental stages. We further use this method to investigate the role of the premotor interneurons in regulating the convulsive behavior caused by a gain-of-function mutation in the neuronal acetylcholine receptor acr-2. Our findings support an instructive role for the interneuron AVB in controlling motor neuron activity and reveal an inhibitory effect of the backward premotor interneurons on the forward interneurons. In summary, the simple inducible cell ablation method reported here allows temporal and spatial control and will prove to be a useful tool in studying the function of specific cells within complex cellular contexts.light inducible | locomotion | optogenetics | reactive oxygen species | pre-motor interneurons

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“…Current techniques such as photo inactivation by light including chromophore assisted light inactivation (CALI), 112,113 transgenically Encoded Protein Photoinactivation (FIAsH-FALI) 114 and Channelrhodopsin-2 (ChR2) 115 as well as the tobacco etch mosaic virus (TEV) protease 116 and auxin 117 based methods have not been widely adapted to questions of mRNA localization. In part, the specificity and speed of these techniques render them incompatible.…”

Section: Future Experimental Approachesmentioning

confidence: 99%

mRNA localization in theDrosophilagermline

Weil

2014

RNA Biology

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Reactive oxygen species in photochemistry of the red fluorescent protein “Killer Red”

Cited by 105 publications

References 30 publications

Mitochondria are targets for peroxisome-derived oxidative stress in cultured mammalian cells

Mitochondria are targets for peroxisome-derived oxidative stress in cultured mammalian cells

Photo-inducible cell ablation in Caenorhabditis elegans using the genetically encoded singlet oxygen generating protein miniSOG

mRNA localization in theDrosophilagermline

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