Reactive Oxygen Species Removal Activity of Davallialactone Reduces Lipopolysaccharide-induced Pulpal Inflammation through Inhibition of the Extracellular Signal-regulated kinase 1/2 and Nuclear Factor kappa B Pathway

An evaluation of the inflammatory response of lipopolysaccharide-treated primary dental pulp cells with regard to calcium silicate-based cements

“…14,29,30 IL-1β production in DP cells can be stimulated using LPS, 14,29 and IL-1β is highly expressed in inflamed pulp tissues. 15,31 Bacterial components might also play an important role in pulpitis.…”

Anti‐inflammatory effect of pachymic acid promotes odontoblastic differentiation via HO‐1 in dental pulp cells

“…Human dental pulp cells (HDPCs) were isolated and cultured, as previously reported (Lee et al , ). When the cells reached confluence, the HDPCs migrating from the tissue were collected and subcultured.…”

“…The cytosolic and nuclear proteins were prepared using a slight modification of the methodology reported elsewhere (Lee et al , ). Briefly, the cells were washed with ice‐cold PBS and scraped into buffer A (10 mM HEPES pH 7.9, 10 mM KCl, 10 mM EDTA, 100 mM dithiothreitol, 10% IGEPAL, 1 mM phenylmethylsulfonyl fluoride, 10 μg ml −1 pepstatin A, 10 μg ml −1 aprotinin, and 10 μg ml −1 leupeptin).…”

Gene Expression Analysis of Resident Macrophages in Lipopolysaccharide-stimulated Rat Molar Pulps

“…Previous reports suggest that TLR4 plays a critical role in the activation of macrophages stimulated by LPS (27). Bacterial components might be a triggering factor in the up-regulation of CD14 and TLR4 in dental pulp tissues, because the up-regulation does not occur in acutely traumatized sterile pulp tissues (28). In this analysis, the up-regulation of both CD14 and TLR4 mRNA was observed in the resident macrophages that were stimulated by LPS.…”